Restriction map for
pF1KB9464
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BamHI BbrPI BfrI
BlpI Bpu1102I BsaAI BsaBI BsiWI
Bsp1407I BspDI BspHI BspMI BsrGI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI Eco47III EcoRI EcoT22I
FseI FspI HincII HindII HpaI
KpnI MamI MluI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1256 GACGTC GACGT^C
AccI 1900 GTAGAC GT^[AC][GT]AC
AcsI 2580 AAATTC [GA]^AATT[CT]
Alw44I 2549 GTGCAC G^TGCAC
AocI 2094 CCTGAGG CC^T[ACGT]AGG
ApaLI 2549 GTGCAC G^TGCAC
ApoI 2580 AAATTC [GA]^AATT[CT]
AvrII 895 CCTAGG C^CTAGG
BbeI 2788 GGCGCC GGCGC^C
BbvCI 570 CCTCAGC CCTCAGC(-5/-2)
BcgI 1583 GCAATGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3453 GGATAC GTATCC(6/5)
BglI 894 GCCTAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 977 AGATCT A^GATCT
BlnI 895 CCTAGG C^CTAGG
BsgI 2866 GTGCAG GTGCAG(16/14)
BsmBI 1254 GAGACG CGTCTC(1/5)
BspLU11I 1079 ACATGT A^CATGT
BssHII 628 GCGCGC G^CGCGC
BstAPI 3307 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1749 GGTGACC G^GT[ACGT]ACC
BstPI 1749 GGTGACC G^GT[ACGT]ACC
Bsu36I 2094 CCTGAGG CC^T[ACGT]AGG
DraIII 2409 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 247 GACAGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2094 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1749 GGTGACC G^GT[ACGT]ACC
EcoRV 3346 GATATC GAT^ATC
HaeII 2788 GGCGCC [GA]GCGC^[CT]
HgaI 534 GCGTC GACGC(5/10)
HindIII 2533 AAGCTT A^AGCTT
KasI 2788 GGCGCC G^GCGCC
NaeI 476 GCCGGC GCC^GGC
NarI 2788 GGCGCC GG^CGCC
NgoMIV 476 GCCGGC G^CCGGC
NheI 2929 GCTAGC G^CTAGC
PflMI 3379 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 73 GAAGAGC GCTCTTC(1/4)
SfoI 2788 GGCGCC GGC^GCC
SphI 2361 GCATGC GCATG^C
SspI 3249 AATATT AAT^ATT
Van91I 3379 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 145 TCCGGA T^CCGGA
452 TCCGGA
AflIII 1079 ACATGT A^C[GA][CT]GT
2545 ACGTGT
BalI 2067 TGGCCA TGG^CCA
3537 TGGCCA
BanI 268 GGCACC G^G[CT][GA]CC
2788 GGCGCC
BclI 2520 TGATCA T^GATCA
2682 TGATCA
BseAI 145 TCCGGA T^CCGGA
452 TCCGGA
BsiEI 723 CGGCCG CG[GA][CT]^CG
904 CGGCCG
BspEI 145 TCCGGA T^CCGGA
452 TCCGGA
BsrDI 1583 GCAATG GCAATG(2/0)
2227 GCAATG
BstYI 977 AGATCT [GA]^GATC[CT]
2296 GGATCT
EagI 723 CGGCCG C^GGCCG
904 CGGCCG
EarI 73 GAAGAG CTCTTC(1/4)
949 GAAGAG
EclXI 723 CGGCCG C^GGCCG
904 CGGCCG
Eco52I 723 CGGCCG C^GGCCG
904 CGGCCG
EcoNI 105 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
396 CCTGGATCAGG
FbaI 2520 TGATCA T^GATCA
2682 TGATCA
Ksp632I 73 GAAGAG CTCTTC(1/4)
949 GAAGAG
KspI 18 CCGCGG CCGC^GG
720 CCGCGG
MfeI 188 CAATTG C^AATTG
2423 CAATTG
MflI 977 AGATCT [GA]^GATC[CT]
2296 GGATCT
MluNI 2067 TGGCCA TGG^CCA
3537 TGGCCA
MroI 145 TCCGGA T^CCGGA
452 TCCGGA
MscI 2067 TGGCCA TGG^CCA
3537 TGGCCA
MslI 270 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2310 CACTGGAGTG
MunI 188 CAATTG C^AATTG
2423 CAATTG
SacI 2909 GAGCTC GAGCT^C
3295 GAGCTC
SacII 18 CCGCGG CCGC^GG
720 CCGCGG
SmaI 210 CCCGGG CCC^GGG
695 CCCGGG
XhoII 977 AGATCT [GA]^GATC[CT]
2296 GGATCT
XmaCI 210 CCCGGG C^CCGGG
695 CCCGGG
XmaI 210 CCCGGG C^CCGGG
695 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI