Restriction map for
pF1KB9461
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbsI BbvCI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NarI NcoI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 12 CCGC CCGC(-3/-1)
AflII 1180 CTTAAG C^TTAAG
AflIII 269 ACATGT A^C[GA][CT]GT
Alw44I 1464 GTGCAC G^TGCAC
AlwNI 628 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1464 GTGCAC G^TGCAC
Asp700I 1197 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 919 CCCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BamHI 164 GGATCC G^GATCC
BanI 694 GGTGCC G^G[CT][GA]CC
BanII 63 GAGCTC G[GA]GC[CT]^C
BcgI 687 CGATGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 435 GGATAC GTATCC(6/5)
BclI 506 TGATCA T^GATCA
BcnI 966 CCGGG CC^[GC]GG
BfrI 1180 CTTAAG C^TTAAG
BglII 1001 AGATCT A^GATCT
BsaWI 1481 TCCGGT [AT]^CCGG[AT]
BsgI 1586 GTGCAG GTGCAG(16/14)
BsoBI 919 CCCGAG C^[CT]CG[GA]G
BspLU11I 269 ACATGT A^CATGT
BsrDI 598 GCAATG GCAATG(2/0)
BsrFI 1241 GCCGGC [GA]^CCGG[CT]
BstXI 698 CCAAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1241 GCCGGC [GA]^CCGG[CT]
DraIII 1458 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1076 CCGTGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
FbaI 506 TGATCA T^GATCA
HincII 462 GTCAAC GT[CT]^[GA]AC
HindII 462 GTCAAC GT[CT]^[GA]AC
MaeII 755 ACGT A^CGT
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NaeI 1241 GCCGGC GCC^GGC
NciI 966 CCGGG CC^[GC]GG
NgoMIV 1241 GCCGGC G^CCGGC
NheI 1420 GCTAGC G^CTAGC
NspI 269 ACATGT [GA]CATG^[CT]
PflMI 316 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 630 GAGTC GAGTC(4/5)
SacI 63 GAGCTC GAGCT^C
ScaI 980 AGTACT AGT^ACT
SexAI 1523 ACCAGGT A^CC[AT]GGT
TaqI 648 TCGA T^CGA
TthHB8I 648 TCGA T^CGA
Van91I 316 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1197 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 63 GAGCTC G[AT]GC[AT]^C
1464 GTGCAC
AvaII 97 GGACC G^G[AT]CC
1545 GGACC
BfaI 928 CTAG C^TAG
1421 CTAG
BmyI 63 GAGCTC G[AGT]GC[ACT]^C
1464 GTGCAC
BsaI 355 GAGACC GGTCTC(1/5)
723 GGTCTC
BseRI 73 GAGGAG GAGGAG(10/8)
76 GAGGAG
BsiHKAI 63 GAGCTC G[AT]GC[AT]^C
1464 GTGCAC
Bsp1286I 63 GAGCTC G[AGT]GC[ACT]^C
1464 GTGCAC
Bsp1407I 272 TGTACA T^GTACA
1571 TGTACA
BsrGI 272 TGTACA T^GTACA
1571 TGTACA
Cac8I 1241 GCCGGC GC[ACGT]^[ACGT]GC
1420 GCTAGC
EarI 70 GAAGAG CTCTTC(1/4)
1114 GAAGAG
EcoRI 365 GAATTC G^AATTC
874 GAATTC
EcoT14I 904 CCTTGG C^C[AT][AT]GG
1088 CCAAGG
HindIII 1576 AAGCTT A^AGCTT
1619 AAGCTT
Ksp632I 70 GAAGAG CTCTTC(1/4)
1114 GAAGAG
MaeI 928 CTAG C^TAG
1421 CTAG
MslI 53 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1371 CACATATGTG
PstI 1252 CTGCAG CTGCA^G
1532 CTGCAG
SmlI 718 CTCAAG C^T[CT][GA]AG
1180 CTTAAG
SspBI 272 TGTACA T^GTACA
1571 TGTACA
StyI 904 CCTTGG C^C[AT][AT]GG
1088 CCAAGG
Tsp45I 1130 GTGAC ^GT[GC]AC
1390 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BspMI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI TfiI Tru9I
TseI Tsp509I TspRI XhoII