Restriction map for
pF1KB9459
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BfrI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BsrBI BsrFI
BssSI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
FspI HgaI KpnI Ksp632I KspI
MamI MluI MroI NaeI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SnaBI SphI Sse8387I SspI
SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1732 GTATAC GT^[AC][GT]AC
AcyI 3377 GGCGCC G[GA]^CG[CT]C
ApaI 3394 GGGCCC GGGCC^C
Asp700I 2140 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 3377 GGCGCC GGCGC^C
BbvCI 792 CCTCAGC CCTCAGC(-5/-2)
BlpI 42 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 42 GCTCAGC GC^T[ACGT]AGC
BsaHI 3377 GGCGCC G[GA]^CG[CT]C
BsiEI 612 CGATCG CG[GA][CT]^CG
BsmBI 198 CGTCTC CGTCTC(1/5)
BssHII 26 GCGCGC G^CGCGC
Bst1107I 1732 GTATAC GTA^TAC
BstEII 3606 GGTCACC G^GT[ACGT]ACC
BstPI 3606 GGTCACC G^GT[ACGT]ACC
BstZ17I 1732 GTATAC GTA^TAC
CelII 42 GCTCAGC GC^T[ACGT]AGC
EcoNI 2043 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3606 GGTCACC G^GT[ACGT]ACC
EcoT22I 1294 ATGCAT ATGCA^T
FauI 30 GCGGG CCCGC(4/6)
Hin1I 3377 GGCGCC G[GA]^CG[CT]C
KasI 3377 GGCGCC G^GCGCC
MfeI 2418 CAATTG C^AATTG
MunI 2418 CAATTG C^AATTG
NarI 3377 GGCGCC GG^CGCC
NcoI 2292 CCATGG C^CATGG
NheI 1147 GCTAGC G^CTAGC
NsiI 1294 ATGCAT ATGCA^T
PpuMI 884 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2258 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 612 CGATCG CGAT^CG
SacI 2659 GAGCTC GAGCT^C
SfoI 3377 GGCGCC GGC^GCC
SmaI 1760 CCCGGG CCC^GGG
SmlI 3343 CTCAAG C^T[CT][GA]AG
StuI 1242 AGGCCT AGG^CCT
XmaCI 1760 CCCGGG C^CCGGG
XmaI 1760 CCCGGG C^CCGGG
XmnI 2140 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AflIII 1496 ACATGT A^C[GA][CT]GT
1503 ACATGT
Alw44I 120 GTGCAC G^TGCAC
3295 GTGCAC
AlwNI 187 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
2911 CAGGCTCTG
AocI 1238 CCTCAGG CC^T[ACGT]AGG
3403 CCTGAGG
ApaLI 120 GTGCAC G^TGCAC
3295 GTGCAC
BalI 1863 TGGCCA TGG^CCA
2190 TGGCCA
BamHI 68 GGATCC G^GATCC
2240 GGATCC
BbsI 439 GAAGAC GAAGAC(2/6)
766 GTCTTC
BciVI 2412 GGATAC GTATCC(6/5)
2437 GTATCC
BpuAI 439 GAAGAC GAAGAC(2/6)
766 GTCTTC
BsaI 16 GAGACC GGTCTC(1/5)
866 GAGACC
BsgI 1834 CTGCAC GTGCAG(16/14)
2393 CTGCAC
Bsp1407I 1138 TGTACA T^GTACA
1178 TGTACA
BspLU11I 1496 ACATGT A^CATGT
1503 ACATGT
BsrDI 263 CATTGC GCAATG(2/0)
2463 CATTGC
BsrGI 1138 TGTACA T^GTACA
1178 TGTACA
BstBI 1682 TTCGAA TT^CGAA
2806 TTCGAA
BstXI 927 CCATGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1994 CCAAAGCACTGG
Bsu36I 1238 CCTCAGG CC^T[ACGT]AGG
3403 CCTGAGG
DraIII 1837 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3557 CACTCAGTG
Eco81I 1238 CCTCAGG CC^T[ACGT]AGG
3403 CCTGAGG
HaeII 248 GGCGCT [GA]GCGC^[CT]
3377 GGCGCC
HapII 735 CCGG C^CGG
1761 CCGG
HincII 3 GTTAAC GT[CT]^[GA]AC
390 GTTAAC
HindII 3 GTTAAC GT[CT]^[GA]AC
390 GTTAAC
HindIII 241 AAGCTT A^AGCTT
3004 AAGCTT
HpaI 3 GTTAAC GTT^AAC
390 GTTAAC
HpaII 735 CCGG C^CGG
1761 CCGG
MluNI 1863 TGGCCA TGG^CCA
2190 TGGCCA
MscI 1863 TGGCCA TGG^CCA
2190 TGGCCA
MspI 735 CCGG C^CGG
1761 CCGG
NspV 1682 TTCGAA TT^CGAA
2806 TTCGAA
PflMI 2061 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3556 CCACTCAGTGG
SfuI 1682 TTCGAA TT^CGAA
2806 TTCGAA
SpeI 76 ACTAGT A^CTAGT
1857 ACTAGT
SspBI 1138 TGTACA T^GTACA
1178 TGTACA
Van91I 2061 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3556 CCACTCAGTGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbvI BclI BcnI
BfaI BglII BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII