Restriction map for
pF1KB9458
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflIII
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfaI
BglI BglII BlnI BsaAI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI MaeI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
SphI SspI StuI SwaI XbaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 673 GACGTC GACGT^C
AflII 2134 CTTAAG C^TTAAG
AocI 251 CCTCAGG CC^T[ACGT]AGG
Aor51HI 384 AGCGCT AGC^GCT
AspI 222 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1565 TGGCCA TGG^CCA
BbvCI 2473 GCTGAGG CCTCAGC(-5/-2)
BclI 1331 TGATCA T^GATCA
BfrI 2134 CTTAAG C^TTAAG
BlpI 2416 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2416 GCTCAGC GC^T[ACGT]AGC
BspHI 1694 TCATGA T^CATGA
BsrBI 2157 CCGCTC CCGCTC(-3/-3)
BsrDI 2031 CATTGC GCAATG(2/0)
BssSI 117 CACGAG CACGAG(-5/-1)
BstEII 645 GGTGACC G^GT[ACGT]ACC
BstPI 645 GGTGACC G^GT[ACGT]ACC
Bsu36I 251 CCTCAGG CC^T[ACGT]AGG
CelII 2416 GCTCAGC GC^T[ACGT]AGC
DraIII 1104 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 384 AGCGCT AGC^GCT
Eco81I 251 CCTCAGG CC^T[ACGT]AGG
EcoO65I 645 GGTGACC G^GT[ACGT]ACC
FbaI 1331 TGATCA T^GATCA
KspI 1662 CCGCGG CCGC^GG
MluNI 1565 TGGCCA TGG^CCA
MscI 1565 TGGCCA TGG^CCA
PaeR7I 2399 CTCGAG C^TCGAG
PflFI 222 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1694 TCATGA T^CATGA
SacI 976 GAGCTC GAGCT^C
SacII 1662 CCGCGG CCGC^GG
SgrAI 197 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2283 CCCGGG CCC^GGG
Tsp509I 1998 AATT ^AATT
Tth111I 222 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2399 CTCGAG C^TCGAG
XmaCI 2283 CCCGGG C^CCGGG
XmaI 2283 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AgeI 198 ACCGGT A^CCGGT
2210 ACCGGT
Alw44I 765 GTGCAC G^TGCAC
1126 GTGCAC
ApaI 401 GGGCCC GGGCC^C
1120 GGGCCC
ApaLI 765 GTGCAC G^TGCAC
1126 GTGCAC
Asp700I 2124 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2148 GAAAGCCTTC
BanI 201 GGTGCC G^G[CT][GA]CC
1085 GGCACC
BsaBI 930 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1677 GATGGGCATC
BsaWI 198 ACCGGT [AT]^CCGG[AT]
2210 ACCGGT
BsmBI 187 GAGACG CGTCTC(1/5)
627 GAGACG
Bsp1407I 920 TGTACA T^GTACA
1325 TGTACA
BsrFI 198 ACCGGT [GA]^CCGG[CT]
2210 ACCGGT
BsrGI 920 TGTACA T^GTACA
1325 TGTACA
Cfr10I 198 ACCGGT [GA]^CCGG[CT]
2210 ACCGGT
DrdI 1276 GACAGATTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2470 GACGCTGAGGTC
HaeII 384 AGCGCT [GA]GCGC^[CT]
829 AGCGCC
HincII 565 GTCAAC GT[CT]^[GA]AC
2053 GTCAAC
HindII 565 GTCAAC GT[CT]^[GA]AC
2053 GTCAAC
MamI 930 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1677 GATGGGCATC
PflMI 305 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1671 CCAGCAGATGG
PinAI 198 ACCGGT A^CCGGT
2210 ACCGGT
PvuII 775 CAGCTG CAG^CTG
1016 CAGCTG
SbfI 1260 CCTGCAGG CCTGCA^GG
1853 CCTGCAGG
Sse8387I 1260 CCTGCAGG CCTGCA^GG
1853 CCTGCAGG
SspBI 920 TGTACA T^GTACA
1325 TGTACA
Van91I 305 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1671 CCAGCAGATGG
XcmI 833 CCAGGGACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2102 CCATCACCATCCTGG
XmnI 2124 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2148 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XhoII