Restriction map for
pF1KB9457
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbrPI BciVI BclI BcnI
BfrI BglII BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstUI Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRV FauI FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MluI MroI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 252 GACGTC GACGT^C
AhdI 542 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 44 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 64 CCTCAGG CC^T[ACGT]AGG
ApaI 834 GGGCCC GGGCC^C
AspEI 542 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 960 GGATCC G^GATCC
BbeI 9 GGCGCC GGCGC^C
BbsI 915 GTCTTC GAAGAC(2/6)
BbvCI 1732 CCTCAGC CCTCAGC(-5/-2)
BcgI 1449 GCACACAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1781 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 915 GTCTTC GAAGAC(2/6)
BsaI 1172 GGTCTC GGTCTC(1/5)
BsaWI 597 ACCGGA [AT]^CCGG[AT]
BsrDI 1746 GCAATG GCAATG(2/0)
Bst1107I 523 GTATAC GTA^TAC
BstAPI 1073 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1702 GGTAACC G^GT[ACGT]ACC
BstPI 1702 GGTAACC G^GT[ACGT]ACC
BstXI 623 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 523 GTATAC GTA^TAC
Bsu36I 64 CCTCAGG CC^T[ACGT]AGG
Eam1105I 542 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 651 CTCTTC CTCTTC(1/4)
Eco81I 64 CCTCAGG CC^T[ACGT]AGG
EcoNI 16 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1702 GGTAACC G^GT[ACGT]ACC
EcoRI 55 GAATTC G^AATTC
EcoT22I 1842 ATGCAT ATGCA^T
HaeII 9 GGCGCC [GA]GCGC^[CT]
HapII 598 CCGG C^CGG
HgaI 2437 GCGTC GACGC(5/10)
HincII 928 GTTGAC GT[CT]^[GA]AC
HindII 928 GTTGAC GT[CT]^[GA]AC
HpaII 598 CCGG C^CGG
KasI 9 GGCGCC G^GCGCC
Ksp632I 651 CTCTTC CTCTTC(1/4)
MaeII 253 ACGT A^CGT
MfeI 1807 CAATTG C^AATTG
MspI 598 CCGG C^CGG
MunI 1807 CAATTG C^AATTG
NarI 9 GGCGCC GG^CGCC
NsiI 1842 ATGCAT ATGCA^T
PstI 1980 CTGCAG CTGCA^G
SacI 405 GAGCTC GAGCT^C
SexAI 1583 ACCAGGT A^CC[AT]GGT
SfcI 1980 CTGCAG C^T[GA][CT]AG
SfoI 9 GGCGCC GGC^GCC
SspI 1504 AATATT AAT^ATT
StuI 733 AGGCCT AGG^CCT
TaqI 409 TCGA T^CGA
TthHB8I 409 TCGA T^CGA
XbaI 667 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 523 GTATAC GT^[AC][GT]AC
2439 GTCTAC
Asp700I 55 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1087 GAATTGGTTC
AspHI 405 GAGCTC G[AT]GC[AT]^C
2122 GAGCAC
AvaI 986 CCCGAG C^[CT]CG[GA]G
1610 CCCGAG
BanI 9 GGCGCC G^G[CT][GA]CC
1211 GGTGCC
BanII 405 GAGCTC G[GA]GC[CT]^C
834 GGGCCC
BbvI 1601 GCAGC GCAGC(8/12)
49 GCTGC
BlpI 373 GCTGAGC GC^T[ACGT]AGC
1534 GCTGAGC
BpmI 1175 CTCCAG CTGGAG(16/14)
2270 CTCCAG
Bpu1102I 373 GCTGAGC GC^T[ACGT]AGC
1534 GCTGAGC
BseRI 1796 CTCCTC GAGGAG(10/8)
2365 GAGGAG
BsiHKAI 405 GAGCTC G[AT]GC[AT]^C
2122 GAGCAC
BsoBI 986 CCCGAG C^[CT]CG[GA]G
1610 CCCGAG
BstYI 483 AGATCC [GA]^GATC[CT]
960 GGATCC
CelII 373 GCTGAGC GC^T[ACGT]AGC
1534 GCTGAGC
DraI 31 TTTAAA TTT^AAA
1671 TTTAAA
MflI 483 AGATCC [GA]^GATC[CT]
960 GGATCC
NcoI 294 CCATGG C^CATGG
1545 CCATGG
PpuMI 176 AGGTCCC [GA]G^G[AT]CC[CT]
1389 AGGACCC
SmlI 965 CTCAAG C^T[CT][GA]AG
1184 CTCAAG
TfiI 306 GAATC G^A[AT]TC
1281 GAATC
TseI 49 GCTGC G^C[AT]GC
1601 GCAGC
XhoII 483 AGATCC [GA]^GATC[CT]
960 GGATCC
XmnI 55 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1087 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AvaII BalI
BfaI BmyI BsaHI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI Hin1I HinP1I HinfI HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I Tsp45I Tsp509I TspRI