Restriction map for
pF1KB9456
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BfrI BlnI BsaAI BsaBI
BsiEI BsmBI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstZ17I Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FseI
FspI HaeII KasI KspI MamI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 2611 CGCG CG^CG
AcsI 393 AAATTT [GA]^AATT[CT]
AcyI 2591 GGCGTC G[GA]^CG[CT]C
AlwNI 3205 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3343 CCTGAGG CC^T[ACGT]AGG
ApoI 393 AAATTT [GA]^AATT[CT]
BamHI 225 GGATCC G^GATCC
BbvCI 102 CCTCAGC CCTCAGC(-5/-2)
BclI 3434 TGATCA T^GATCA
BglI 2540 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2428 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2428 GCTGAGC GC^T[ACGT]AGC
BsaHI 2591 GGCGTC G[GA]^CG[CT]C
BsiWI 1008 CGTACG C^GTACG
BsmI 1301 GCATTC GAATGC(1/-1)
BspDI 64 ATCGAT AT^CGAT
BspMI 3209 ACCTGC ACCTGC(4/8)
BstAPI 211 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2000 TTCGAA TT^CGAA
BstEII 1132 GGTAACC G^GT[ACGT]ACC
BstPI 1132 GGTAACC G^GT[ACGT]ACC
BstUI 2611 CGCG CG^CG
Bsu36I 3343 CCTGAGG CC^T[ACGT]AGG
CelII 2428 GCTGAGC GC^T[ACGT]AGC
ClaI 64 ATCGAT AT^CGAT
DrdI 2302 GACTTACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3343 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1132 GGTAACC G^GT[ACGT]ACC
FauI 2609 CCCGC CCCGC(4/6)
FbaI 3434 TGATCA T^GATCA
Hin1I 2591 GGCGTC G[GA]^CG[CT]C
HindIII 2417 AAGCTT A^AGCTT
HpaI 3283 GTTAAC GTT^AAC
MvnI 2611 CGCG CG^CG
NspV 2000 TTCGAA TT^CGAA
PaeR7I 1233 CTCGAG C^TCGAG
SfuI 2000 TTCGAA TT^CGAA
SphI 1124 GCATGC GCATG^C
StuI 2266 AGGCCT AGG^CCT
XhoI 1233 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 556 GGTACC G^GTACC
823 GGTACC
AccIII 96 TCCGGA T^CCGGA
3225 TCCGGA
AflIII 1738 ACATGT A^C[GA][CT]GT
2307 ACATGT
Alw44I 1769 GTGCAC G^TGCAC
3133 GTGCAC
ApaI 780 GGGCCC GGGCC^C
2042 GGGCCC
ApaLI 1769 GTGCAC G^TGCAC
3133 GTGCAC
Asp700I 865 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1423 GAATATGTTC
Asp718I 556 GGTACC G^GTACC
823 GGTACC
AspHI 1769 GTGCAC G[AT]GC[AT]^C
3133 GTGCAC
BciVI 2186 GTATCC GTATCC(6/5)
2858 GTATCC
BglII 1513 AGATCT A^GATCT
1521 AGATCT
BsaI 2016 GAGACC GGTCTC(1/5)
2362 GGTCTC
BseAI 96 TCCGGA T^CCGGA
3225 TCCGGA
BsgI 2207 GTGCAG GTGCAG(16/14)
2814 GTGCAG
BsiHKAI 1769 GTGCAC G[AT]GC[AT]^C
3133 GTGCAC
Bsp1407I 688 TGTACA T^GTACA
845 TGTACA
BspEI 96 TCCGGA T^CCGGA
3225 TCCGGA
BspLU11I 1738 ACATGT A^CATGT
2307 ACATGT
BsrGI 688 TGTACA T^GTACA
845 TGTACA
CfoI 2612 GCGC GCG^C
2967 GCGC
DraIII 230 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2797 CACAAGGTG
EarI 499 GAAGAG CTCTTC(1/4)
3106 GAAGAG
EcoNI 1353 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2448 CCTAATGGAGG
HhaI 2612 GCGC GCG^C
2967 GCGC
HinP1I 2612 GCGC G^CGC
2967 GCGC
KpnI 556 GGTACC GGTAC^C
823 GGTACC
Ksp632I 499 GAAGAG CTCTTC(1/4)
3106 GAAGAG
MaeII 1163 ACGT A^CGT
1283 ACGT
MfeI 665 CAATTG C^AATTG
3046 CAATTG
MroI 96 TCCGGA T^CCGGA
3225 TCCGGA
MunI 665 CAATTG C^AATTG
3046 CAATTG
NcoI 1705 CCATGG C^CATGG
2117 CCATGG
PflMI 784 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2481 CCAGCTGGTGG
PshAI 88 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
271 GACCTGAGTC
SspBI 688 TGTACA T^GTACA
845 TGTACA
SspI 380 AATATT AAT^ATT
592 AATATT
Van91I 784 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2481 CCAGCTGGTGG
XmnI 865 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1423 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI