Restriction map for
pF1KB9454
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 49 GTCTAC GT^[AC][GT]AC
AciI 198 GCGG CCGC(-3/-1)
AcsI 450 AAATTT [GA]^AATT[CT]
AflIII 1069 ACATGT A^C[GA][CT]GT
ApoI 450 AAATTT [GA]^AATT[CT]
Asp700I 544 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 805 GGACC G^G[AT]CC
BbvCI 784 GCTGAGG CCTCAGC(-5/-2)
BcnI 1082 CCCGG CC^[GC]GG
BfaI 982 CTAG C^TAG
BsaAI 1228 TACGTA [CT]AC^GT[GA]
BsaI 827 GAGACC GGTCTC(1/5)
BsaWI 6 TCCGGT [AT]^CCGG[AT]
BsgI 665 GTGCAG GTGCAG(16/14)
BsmI 997 GAATGC GAATGC(1/-1)
Bsp1407I 507 TGTACA T^GTACA
BspLU11I 1069 ACATGT A^CATGT
BspMI 667 GCAGGT ACCTGC(4/8)
BsrGI 507 TGTACA T^GTACA
Cfr13I 805 GGACC G^G[ACGT]CC
DraI 88 TTTAAA TTT^AAA
DrdI 1456 GACAATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 279 CCTTGG C^C[AT][AT]GG
HaeIII 814 GGCC GG^CC
MaeI 982 CTAG C^TAG
NciI 1082 CCCGG CC^[GC]GG
NspI 1069 ACATGT [GA]CATG^[CT]
PflFI 829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1358 GAGCTC GAGCT^C
Sau96I 805 GGACC G^G[ACGT]CC
SnaBI 1228 TACGTA TAC^GTA
SspBI 507 TGTACA T^GTACA
StuI 813 AGGCCT AGG^CCT
StyI 279 CCTTGG C^C[AT][AT]GG
TaqI 552 TCGA T^CGA
Tth111I 829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 552 TCGA T^CGA
XcmI 1520 CCAATGTGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 544 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 766 GTGCTC G[AT]GC[AT]^C
1358 GAGCTC
BanII 907 GAGCCC G[GA]GC[CT]^C
1358 GAGCTC
BbsI 103 GAAGAC GAAGAC(2/6)
1073 GTCTTC
BglII 1305 AGATCT A^GATCT
1470 AGATCT
BpmI 593 CTGGAG CTGGAG(16/14)
755 CTGGAG
BpuAI 103 GAAGAC GAAGAC(2/6)
1073 GTCTTC
BsiHKAI 766 GTGCTC G[AT]GC[AT]^C
1358 GAGCTC
BsmFI 192 GGGAC GGGAC(10/14)
1533 GGGAC
BstYI 1305 AGATCT [GA]^GATC[CT]
1470 AGATCT
HapII 7 CCGG C^CGG
1083 CCGG
HpaII 7 CCGG C^CGG
1083 CCGG
MaeII 1229 ACGT A^CGT
1284 ACGT
MflI 1305 AGATCT [GA]^GATC[CT]
1470 AGATCT
MslI 94 CATTCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1020 CACAGATGTG
MspI 7 CCGG C^CGG
1083 CCGG
NlaIV 438 GGTTCC GG[ACGT]^[ACGT]CC
805 GGACCC
PshAI 42 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1326 GACATTTGTC
SfcI 844 CTACAG C^T[GA][CT]AG
1038 CTACAG
SmlI 1103 CTCAAG C^T[CT][GA]AG
1140 CTCAAG
TfiI 3 GATTC G^A[AT]TC
1410 GATTC
Tsp45I 835 GTCAC ^GT[GC]AC
1525 GTGAC
XhoII 1305 AGATCT [GA]^GATC[CT]
1470 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI DdeI
DpnII EarI EcoRII Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII PleI RsaI
Sau3AI ScrFI SfaNI Tru9I TseI
Tsp509I TspRI