Restriction map for
pF1KB9446
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KspI MaeI MaeIII
MamI MfeI MluI MluNI MscI
MspA1I MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 688 GGTACC G^GTACC
AccIII 171 TCCGGA T^CCGGA
AciI 637 GCGG CCGC(-3/-1)
AcyI 3 GGCGCC G[GA]^CG[CT]C
AhdI 291 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 421 GATCC GGATC(4/5)
ApaI 410 GGGCCC GGGCC^C
Asp718I 688 GGTACC G^GTACC
AspEI 291 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 413 CCCGGG C^[CT]CG[GA]G
AvaII 15 GGACC G^G[AT]CC
BanII 410 GGGCCC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BbsI 288 GAAGAC GAAGAC(2/6)
BbvCI 388 CCTCAGC CCTCAGC(-5/-2)
BbvI 274 GCTGC GCAGC(8/12)
BcgI 46 CGAGTGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 410 GGGCCC G[AGT]GC[ACT]^C
BpuAI 288 GAAGAC GAAGAC(2/6)
BsaAI 193 TACGTG [CT]AC^GT[GA]
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaWI 171 TCCGGA [AT]^CCGG[AT]
BseAI 171 TCCGGA T^CCGGA
BsiYI 352 CCTGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 352 CCTGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 413 CCCGGG C^[CT]CG[GA]G
Bsp1286I 410 GGGCCC G[AGT]GC[ACT]^C
BspEI 171 TCCGGA T^CCGGA
BspHI 449 TCATGA T^CATGA
BsrDI 814 GCAATG GCAATG(2/0)
BssSI 608 CTCGTG CACGAG(-5/-1)
BstXI 765 CCAGGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 420 AGATCC [GA]^GATC[CT]
Cac8I 922 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 496 CCGTGG C^C[GA][CT]GG
Eam1105I 291 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 900 GAATTC G^AATTC
Fnu4HI 274 GCTGC GC^[ACGT]GC
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HphI 40 GGTGA GGTGA(8/7)
ItaI 274 GCTGC GC^[ACGT]GC
KasI 3 GGCGCC G^GCGCC
KpnI 688 GGTACC GGTAC^C
MflI 420 AGATCC [GA]^GATC[CT]
MroI 171 TCCGGA T^CCGGA
NarI 3 GGCGCC GG^CGCC
PpuMI 14 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 449 TCATGA T^CATGA
ScaI 183 AGTACT AGT^ACT
SfaNI 751 GATGC GCATC(5/9)
SfoI 3 GGCGCC GGC^GCC
SmaI 413 CCCGGG CCC^GGG
SmlI 580 CTTGAG C^T[CT][GA]AG
TseI 274 GCTGC G^C[AT]GC
XhoII 420 AGATCC [GA]^GATC[CT]
XmaCI 413 CCCGGG C^CCGGG
XmaI 413 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 120 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
163 GAATGGGTTC
BanI 3 GGCGCC G^G[CT][GA]CC
688 GGTACC
BcnI 413 CCCGG CC^[GC]GG
414 CCGGG
BsaI 382 GAGACC GGTCTC(1/5)
714 GAGACC
BseRI 395 CTCCTC GAGGAG(10/8)
941 CTCCTC
BsmAI 382 GAGAC GTCTC(1/5)
714 GAGAC
BsmFI 515 GGGAC GGGAC(10/14)
762 GTCCC
BsrI 512 ACTGG ACTGG(1/-1)
377 CCAGT
CfoI 4 GCGC GCG^C
778 GCGC
DpnII 421 GATC ^GATC
742 GATC
DraII 14 AGGACCC [GA]G^G[ACGT]CC[CT]
409 AGGGCCC
EcoO109I 14 AGGACCC [GA]G^G[ACGT]CC[CT]
409 AGGGCCC
HaeII 3 GGCGCC [GA]GCGC^[CT]
777 AGCGCC
HaeIII 65 GGCC GG^CC
411 GGCC
HapII 172 CCGG C^CGG
414 CCGG
HhaI 4 GCGC GCG^C
778 GCGC
HinP1I 4 GCGC G^CGC
778 GCGC
HinfI 477 GATTC G^A[ACGT]TC
571 GATTC
HpaII 172 CCGG C^CGG
414 CCGG
MaeII 194 ACGT A^CGT
677 ACGT
MboI 421 GATC ^GATC
742 GATC
MslI 133 CATTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
745 CATCCCGATG
MspI 172 CCGG C^CGG
414 CCGG
NciI 413 CCCGG CC^[GC]GG
414 CCGGG
NdeII 421 GATC ^GATC
742 GATC
SapI 484 GAAGAGC GCTCTTC(1/4)
843 GAAGAGC
Sau3AI 421 GATC ^GATC
742 GATC
TfiI 477 GATTC G^A[AT]TC
571 GATTC
XmnI 120 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
163 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsaJI
BssKI BstF5I BstNI Cfr13I DdeI
EarI EcoRII FokI Ksp632I MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI Tru9I
Tsp509I TspRI