##################################
# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI ItaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MluNI MscI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 38 TCCGGA T^CCGGA
AcsI 441 AAATTT [GA]^AATT[CT]
AocI 336 CCTTAGG CC^T[ACGT]AGG
ApoI 441 AAATTT [GA]^AATT[CT]
Asp700I 286 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 36 GGTCC G^G[AT]CC
BbsI 397 GAAGAC GAAGAC(2/6)
BglII 429 AGATCT A^GATCT
BpuAI 397 GAAGAC GAAGAC(2/6)
BsaJI 361 CCACGG C^C[ACGT][ACGT]GG
BsaWI 38 TCCGGA [AT]^CCGG[AT]
BseAI 38 TCCGGA T^CCGGA
BsiYI 39 CCGGATAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 39 CCGGATAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 190 GGGAC GGGAC(10/14)
BspEI 38 TCCGGA T^CCGGA
BssKI 295 CCTGG ^CC[ACGT]GG
BstNI 295 CCTGG CC^[AT]GG
BstYI 429 AGATCT [GA]^GATC[CT]
Bsu36I 336 CCTTAGG CC^T[ACGT]AGG
Cac8I 61 GCTCGC GC[ACGT]^[ACGT]GC
Cfr13I 36 GGTCC G^G[ACGT]CC
DpnII 430 GATC ^GATC
DsaI 361 CCACGG C^C[GA][CT]GG
EarI 163 GAAGAG CTCTTC(1/4)
Eco81I 336 CCTTAGG CC^T[ACGT]AGG
EcoRII 295 CCTGG ^CC[AT]GG
HapII 39 CCGG C^CGG
HinfI 3 GAATC G^A[ACGT]TC
HpaII 39 CCGG C^CGG
HphI 168 GGTGA GGTGA(8/7)
Ksp632I 163 GAAGAG CTCTTC(1/4)
MboI 430 GATC ^GATC
MflI 429 AGATCT [GA]^GATC[CT]
MroI 38 TCCGGA T^CCGGA
MslI 282 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 98 CAGCTG C[AC]G^C[GT]G
MspI 39 CCGG C^CGG
MvaI 295 CCTGG CC^[AT]GG
NdeII 430 GATC ^GATC
PvuII 98 CAGCTG CAG^CTG
Sau3AI 430 GATC ^GATC
Sau96I 36 GGTCC G^G[ACGT]CC
ScrFI 295 CCTGG CC^[ACGT]GG
SfaNI 454 GCATC GCATC(5/9)
SspI 300 AATATT AAT^ATT
TfiI 3 GAATC G^A[AT]TC
XhoII 429 AGATCT [GA]^GATC[CT]
XmnI 286 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 7 enzymes) #
###################################
AfaI 56 GTAC GT^AC
82 GTAC
BsrI 135 ACTGG ACTGG(1/-1)
256 ACTGG
DdeI 32 CTCAG C^T[ACGT]AG
337 CTTAG
HindIII 112 AAGCTT A^AGCTT
224 AAGCTT
NlaIII 174 CATG CATG^
262 CATG
RsaI 56 GTAC GT^AC
82 GTAC
Tsp509I 252 AATT ^AATT
442 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
AluI MboII MnlI MseI Tru9I