Restriction map for
pF1KB9433
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BmyI BsaBI BsaI BseAI BsgI
BsiHKAI BsiWI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstF5I BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MluNI MroI
MscI MslI MunI NarI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 795 GGCGTC G[GA]^CG[CT]C
AflIII 519 ACGCGT A^C[GA][CT]GT
AlwI 78 GGATC GGATC(4/5)
Aor51HI 224 AGCGCT AGC^GCT
BanI 107 GGCACC G^G[CT][GA]CC
BbsI 801 GTCTTC GAAGAC(2/6)
BglI 787 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 448 CTGGAG CTGGAG(16/14)
BpuAI 801 GTCTTC GAAGAC(2/6)
BsaAI 436 TACGTG [CT]AC^GT[GA]
BsaHI 795 GGCGTC G[GA]^CG[CT]C
BsaWI 99 ACCGGA [AT]^CCGG[AT]
BsiEI 398 CGGCCG CG[GA][CT]^CG
BsmBI 37 GAGACG CGTCTC(1/5)
BsrBI 606 GAGCGG CCGCTC(-3/-3)
BstAPI 696 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 658 GGTGACC G^GT[ACGT]ACC
BstPI 658 GGTGACC G^GT[ACGT]ACC
BstYI 78 GGATCT [GA]^GATC[CT]
DpnII 79 GATC ^GATC
DsaI 206 CCGCGG C^C[GA][CT]GG
EaeI 398 CGGCCG [CT]^GGCC[GA]
EagI 398 CGGCCG C^GGCCG
EclXI 398 CGGCCG C^GGCCG
Eco47III 224 AGCGCT AGC^GCT
Eco52I 398 CGGCCG C^GGCCG
EcoO65I 658 GGTGACC G^GT[ACGT]ACC
HgaI 796 GCGTC GACGC(5/10)
Hin1I 795 GGCGTC G[GA]^CG[CT]C
HphI 658 GGTGA GGTGA(8/7)
KspI 206 CCGCGG CCGC^GG
MaeII 437 ACGT A^CGT
MboI 79 GATC ^GATC
MflI 78 GGATCT [GA]^GATC[CT]
MluI 519 ACGCGT A^CGCGT
NaeI 599 GCCGGC GCC^GGC
NdeII 79 GATC ^GATC
NgoMIV 599 GCCGGC G^CCGGC
NlaIII 742 CATG CATG^
NotI 397 GCGGCCGC GC^GGCCGC
PpuMI 698 AGGACCT [GA]G^G[AT]CC[CT]
SacII 206 CCGCGG CCGC^GG
SapI 636 GCTCTTC GCTCTTC(1/4)
Sau3AI 79 GATC ^GATC
SgrAI 654 CGCCGGTG C[GA]^CCGG[CT]G
TfiI 501 GAATC G^A[AT]TC
XhoII 78 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 207 CGCG CG^CG
520 CGCG
AfaI 435 GTAC GT^AC
523 GTAC
AhdI 52 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
372 GACAAAGAGTC
AspEI 52 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
372 GACAAAGAGTC
AvaI 27 CCCGGG C^[CT]CG[GA]G
118 CCCGGG
BsoBI 27 CCCGGG C^[CT]CG[GA]G
118 CCCGGG
BspMI 236 ACCTGC ACCTGC(4/8)
701 ACCTGC
BstUI 207 CGCG CG^CG
520 CGCG
DraII 135 GGGGCCT [GA]G^G[ACGT]CC[CT]
698 AGGACCT
Eam1105I 52 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
372 GACAAAGAGTC
EcoO109I 135 GGGGCCT [GA]G^G[ACGT]CC[CT]
698 AGGACCT
HaeII 224 AGCGCT [GA]GCGC^[CT]
295 AGCGCC
MvnI 207 CGCG CG^CG
520 CGCG
PstI 270 CTGCAG CTGCA^G
532 CTGCAG
RsaI 435 GTAC GT^AC
523 GTAC
SfcI 270 CTGCAG C^T[GA][CT]AG
532 CTGCAG
SmaI 27 CCCGGG CCC^GGG
118 CCCGGG
TaqI 417 TCGA T^CGA
456 TCGA
Tsp45I 284 GTGAC ^GT[GC]AC
659 GTGAC
TthHB8I 417 TCGA T^CGA
456 TCGA
XmaCI 27 CCCGGG C^CCGGG
118 CCCGGG
XmaI 27 CCCGGG C^CCGGG
118 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BlpI Bpu1102I BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsrFI
BsrI BssKI BstNI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI EarI
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NlaIV PleI Sau96I ScrFI Tru9I
TseI TspRI