Restriction map for
pF1KB9422
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MaeI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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ApaI 598 GGGCCC GGGCC^C
Asp700I 651 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1693 CACGTG CAC^GTG
BbsI 1501 GAAGAC GAAGAC(2/6)
BbvCI 1597 GCTGAGG CCTCAGC(-5/-2)
BglII 631 AGATCT A^GATCT
BlpI 949 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 949 GCTGAGC GC^T[ACGT]AGC
BpuAI 1501 GAAGAC GAAGAC(2/6)
BsaAI 1693 CACGTG [CT]AC^GT[GA]
BstYI 631 AGATCT [GA]^GATC[CT]
CelII 949 GCTGAGC GC^T[ACGT]AGC
DpnII 632 GATC ^GATC
EcoT22I 1187 ATGCAT ATGCA^T
HgaI 285 GCGTC GACGC(5/10)
HincII 672 GTTAAC GT[CT]^[GA]AC
HindII 672 GTTAAC GT[CT]^[GA]AC
HindIII 1165 AAGCTT A^AGCTT
HpaI 672 GTTAAC GTT^AAC
MboI 632 GATC ^GATC
MfeI 1199 CAATTG C^AATTG
MflI 631 AGATCT [GA]^GATC[CT]
MunI 1199 CAATTG C^AATTG
NdeII 632 GATC ^GATC
NsiI 1187 ATGCAT ATGCA^T
NspI 1082 GCATGC [GA]CATG^[CT]
PmaCI 1693 CACGTG CAC^GTG
PmlI 1693 CACGTG CAC^GTG
SacI 136 GAGCTC GAGCT^C
SapI 325 GAAGAGC GCTCTTC(1/4)
Sau3AI 632 GATC ^GATC
SphI 1082 GCATGC GCATG^C
SspI 1627 AATATT AAT^ATT
XhoII 631 AGATCT [GA]^GATC[CT]
XmnI 651 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1002 AAATTC [GA]^AATT[CT]
1433 AAATTC
AflIII 98 ACACGT A^C[GA][CT]GT
1679 ACACGT
AlwNI 685 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1605 CAGGGACTG
AocI 699 CCTAAGG CC^T[ACGT]AGG
1371 CCTCAGG
ApoI 1002 AAATTC [GA]^AATT[CT]
1433 AAATTC
AspHI 136 GAGCTC G[AT]GC[AT]^C
822 GAGCAC
BcnI 108 CCCGG CC^[GC]GG
855 CCCGG
BsiHKAI 136 GAGCTC G[AT]GC[AT]^C
822 GAGCAC
BsmFI 1607 GGGAC GGGAC(10/14)
3 GTCCC
BspHI 303 TCATGA T^CATGA
1437 TCATGA
BsrDI 1332 CATTGC GCAATG(2/0)
1460 CATTGC
BssSI 603 CACGAG CACGAG(-5/-1)
624 CACGAG
BstBI 658 TTCGAA TT^CGAA
1498 TTCGAA
Bsu36I 699 CCTAAGG CC^T[ACGT]AGG
1371 CCTCAGG
Eco81I 699 CCTAAGG CC^T[ACGT]AGG
1371 CCTCAGG
HapII 109 CCGG C^CGG
856 CCGG
HpaII 109 CCGG C^CGG
856 CCGG
HphI 441 GGTGA GGTGA(8/7)
897 GGTGA
MslI 304 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1180 CATAATAATG
MspI 109 CCGG C^CGG
856 CCGG
NciI 108 CCCGG CC^[GC]GG
855 CCCGG
NspV 658 TTCGAA TT^CGAA
1498 TTCGAA
RcaI 303 TCATGA T^CATGA
1437 TCATGA
SfuI 658 TTCGAA TT^CGAA
1498 TTCGAA
TaqI 659 TCGA T^CGA
1499 TCGA
Tsp45I 898 GTGAC ^GT[GC]AC
1559 GTGAC
TthHB8I 659 TCGA T^CGA
1499 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeII MaeIII MboII MnlI MseI
MspA1I MvaI MwoI NlaIII NlaIV
PleI PpuMI PstI PvuII Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp509I TspRI