Restriction map for
pF1KB9419
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BanII BbeI BbrPI
BbvCI BcgI BclI BcnI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 1078 CTTAAG C^TTAAG
AhdI 783 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 783 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 680 GAGCAC G[AT]GC[AT]^C
AvaII 1533 GGACC G^G[AT]CC
BamHI 190 GGATCC G^GATCC
BanI 240 GGTGCC G^G[CT][GA]CC
BfrI 1078 CTTAAG C^TTAAG
BglI 1478 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1185 AGATCT A^GATCT
BpmI 62 CTCCAG CTGGAG(16/14)
BsgI 1328 CTGCAC GTGCAG(16/14)
BsiHKAI 680 GAGCAC G[AT]GC[AT]^C
BstAPI 796 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1303 CCAATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 444 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 442 GCGC GCG^C
DraII 1532 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 783 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 208 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1532 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1376 GAATTC G^AATTC
HaeII 441 AGCGCC [GA]GCGC^[CT]
HhaI 442 GCGC GCG^C
HinP1I 442 GCGC G^CGC
HincII 750 GTTGAC GT[CT]^[GA]AC
HindII 750 GTTGAC GT[CT]^[GA]AC
MspA1I 1114 CAGCTG C[AC]G^C[GT]G
NspI 726 ACATGC [GA]CATG^[CT]
PaeR7I 818 CTCGAG C^TCGAG
PleI 783 GACTC GAGTC(4/5)
PpuMI 1532 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1114 CAGCTG CAG^CTG
ScaI 1440 AGTACT AGT^ACT
SexAI 924 ACCAGGT A^CC[AT]GGT
SpeI 1392 ACTAGT A^CTAGT
SspI 1201 AATATT AAT^ATT
TaqI 819 TCGA T^CGA
TthHB8I 819 TCGA T^CGA
XhoI 818 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 191 GATCC GGATC(4/5)
190 GGATC
AlwNI 65 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1316 CAGCTCCTG
AocI 194 CCTCAGG CC^T[ACGT]AGG
1480 CCTCAGG
Asp700I 346 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1558 GAATATATTC
AvaI 426 CCCGAG C^[CT]CG[GA]G
818 CTCGAG
BciVI 661 GGATAC GTATCC(6/5)
1462 GGATAC
BmyI 680 GAGCAC G[AGT]GC[ACT]^C
1476 GTGCCC
BsoBI 426 CCCGAG C^[CT]CG[GA]G
818 CTCGAG
Bsp1286I 680 GAGCAC G[AGT]GC[ACT]^C
1476 GTGCCC
BstYI 190 GGATCC [GA]^GATC[CT]
1185 AGATCT
Bsu36I 194 CCTCAGG CC^T[ACGT]AGG
1480 CCTCAGG
Cfr13I 1404 GGGCC G^G[ACGT]CC
1533 GGACC
Eco81I 194 CCTCAGG CC^T[ACGT]AGG
1480 CCTCAGG
HaeIII 291 GGCC GG^CC
1405 GGCC
MflI 190 GGATCC [GA]^GATC[CT]
1185 AGATCT
MslI 108 CACTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
727 CATGCTGATG
PstI 446 CTGCAG CTGCA^G
697 CTGCAG
Sau96I 1404 GGGCC G^G[ACGT]CC
1533 GGACC
XhoII 190 GGATCC [GA]^GATC[CT]
1185 AGATCT
XmnI 346 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1558 GAATATATTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BbvI BfaI BpuAI BsiYI BslI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HindIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI