Restriction map for
pF1KB9418
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlpI
Bpu1102I BsaAI BsaHI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MluI MluNI MscI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 1148 TCCGGA T^CCGGA
AciI 1471 GCGG CCGC(-3/-1)
AflIII 111 ACATGT A^C[GA][CT]GT
AlwNI 1376 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 848 AGCGCT AGC^GCT
ApaI 1497 GGGCCC GGGCC^C
Asp700I 109 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 797 GAGCAC G[AT]GC[AT]^C
AvrII 1297 CCTAGG C^CTAGG
BamHI 906 GGATCC G^GATCC
BbsI 927 GTCTTC GAAGAC(2/6)
BbvCI 304 GCTGAGG CCTCAGC(-5/-2)
BcnI 490 CCGGG CC^[GC]GG
BglII 882 AGATCT A^GATCT
BlnI 1297 CCTAGG C^CTAGG
BpmI 652 CTCCAG CTGGAG(16/14)
BpuAI 927 GTCTTC GAAGAC(2/6)
BsaBI 901 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 784 GAGACC GGTCTC(1/5)
BsaWI 1148 TCCGGA [AT]^CCGG[AT]
BseAI 1148 TCCGGA T^CCGGA
BsiEI 982 CGGTCG CG[GA][CT]^CG
BsiHKAI 797 GAGCAC G[AT]GC[AT]^C
BsmFI 1183 GTCCC GGGAC(10/14)
BspEI 1148 TCCGGA T^CCGGA
BspHI 267 TCATGA T^CATGA
BspLU11I 111 ACATGT A^CATGT
BstEII 1602 GGTGACC G^GT[ACGT]ACC
BstPI 1602 GGTGACC G^GT[ACGT]ACC
CfoI 849 GCGC GCG^C
DsaI 788 CCATGG C^C[GA][CT]GG
Eco47III 848 AGCGCT AGC^GCT
EcoO65I 1602 GGTGACC G^GT[ACGT]ACC
EcoRI 1044 GAATTC G^AATTC
FauI 1471 GCGGG CCCGC(4/6)
HaeII 848 AGCGCT [GA]GCGC^[CT]
HhaI 849 GCGC GCG^C
HinP1I 849 GCGC G^CGC
HindIII 257 AAGCTT A^AGCTT
MaeII 50 ACGT A^CGT
MamI 901 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1148 TCCGGA T^CCGGA
MslI 411 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1627 CAGCTG C[AC]G^C[GT]G
NciI 490 CCGGG CC^[GC]GG
NcoI 788 CCATGG C^CATGG
NspI 111 ACATGT [GA]CATG^[CT]
PflMI 740 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1517 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1456 CTGCAG CTGCA^G
PvuII 1627 CAGCTG CAG^CTG
RcaI 267 TCATGA T^CATGA
ScaI 1434 AGTACT AGT^ACT
SmlI 1676 CTCAAG C^T[CT][GA]AG
TaqI 985 TCGA T^CGA
TthHB8I 985 TCGA T^CGA
Van91I 740 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1064 CCAAAGATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 109 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 689 GGACC G^G[AT]CC
1518 GGTCC
BsgI 1592 CTGCAC GTGCAG(16/14)
1111 GTGCAG
BsrDI 1529 GCAATG GCAATG(2/0)
1547 GCAATG
BsrFI 145 GCCGGT [GA]^CCGG[CT]
980 GCCGGT
Cac8I 1357 GCCAGC GC[ACGT]^[ACGT]GC
1471 GCGGGC
Cfr10I 145 GCCGGT [GA]^CCGG[CT]
980 GCCGGT
DraI 712 TTTAAA TTT^AAA
865 TTTAAA
DraII 1497 GGGCCCT [GA]G^G[ACGT]CC[CT]
1517 AGGTCCT
EcoO109I 1497 GGGCCCT [GA]G^G[ACGT]CC[CT]
1517 AGGTCCT
EcoT14I 788 CCATGG C^C[AT][AT]GG
1297 CCTAGG
HaeIII 334 GGCC GG^CC
1498 GGCC
MfeI 284 CAATTG C^AATTG
1487 CAATTG
MunI 284 CAATTG C^AATTG
1487 CAATTG
PleI 484 GACTC GAGTC(4/5)
925 GAGTC
SfcI 1025 CTACAG C^T[GA][CT]AG
1456 CTGCAG
StyI 788 CCATGG C^C[AT][AT]GG
1297 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BanII BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI SapI Sau3AI
Sau96I ScrFI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII