Restriction map for
pF1KB9417
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AvrII BamHI BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KspI
MfeI MluI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI ScaI SfiI
SfuI SnaBI SpeI SspBI SspI
SwaI TaqI Tru9I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 2146 GTCTAC GT^[AC][GT]AC
AcsI 1744 GAATTT [GA]^AATT[CT]
AflIII 2021 ACATGT A^C[GA][CT]GT
AhdI 2564 GACGCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 222 GATCC GGATC(4/5)
AocI 2515 CCTAAGG CC^T[ACGT]AGG
ApoI 1744 GAATTT [GA]^AATT[CT]
AspEI 2564 GACGCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2140 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1472 TGCGCA TGC^GCA
BalI 539 TGGCCA TGG^CCA
BbvCI 2545 GCTGAGG CCTCAGC(-5/-2)
BglI 743 GCCTGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2060 AGATCT A^GATCT
BlpI 2602 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2602 GCTCAGC GC^T[ACGT]AGC
BsmBI 979 GAGACG CGTCTC(1/5)
BspLU11I 2021 ACATGT A^CATGT
BspMI 1901 ACCTGC ACCTGC(4/8)
BstAPI 780 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2515 CCTAAGG CC^T[ACGT]AGG
CelII 2602 GCTCAGC GC^T[ACGT]AGC
DraIII 1573 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 372 GACCTTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2564 GACGCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2515 CCTAAGG CC^T[ACGT]AGG
EcoNI 261 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 629 CCAAGG C^C[AT][AT]GG
FspI 1472 TGCGCA TGC^GCA
HincII 2437 GTCAAC GT[CT]^[GA]AC
HindII 2437 GTCAAC GT[CT]^[GA]AC
MluNI 539 TGGCCA TGG^CCA
MscI 539 TGGCCA TGG^CCA
PflFI 2140 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 345 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 229 GAGCTC GAGCT^C
SapI 1531 GAAGAGC GCTCTTC(1/4)
SexAI 695 ACCTGGT A^CC[AT]GGT
SgrAI 1390 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1657 CTCAAG C^T[CT][GA]AG
StyI 629 CCAAGG C^C[AT][AT]GG
Tsp509I 1745 AATT ^AATT
Tth111I 2140 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 345 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1652 CCATCCTCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
ApaI 945 GGGCCC GGGCC^C
1002 GGGCCC
AspHI 229 GAGCTC G[AT]GC[AT]^C
667 GTGCTC
BbeI 1071 GGCGCC GGCGC^C
1277 GGCGCC
BsaBI 804 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1683 GATTGCCATC
BsgI 771 CTGCAC GTGCAG(16/14)
1692 CTGCAC
BsiHKAI 229 GAGCTC G[AT]GC[AT]^C
667 GTGCTC
BspHI 1763 TCATGA T^CATGA
1838 TCATGA
BsrBI 2600 CCGCTC CCGCTC(-3/-3)
747 GAGCGG
BssSI 1695 CACGAG CACGAG(-5/-1)
1311 CTCGTG
BstEII 636 GGTCACC G^GT[ACGT]ACC
1164 GGTGACC
BstPI 636 GGTCACC G^GT[ACGT]ACC
1164 GGTGACC
BstYI 221 AGATCC [GA]^GATC[CT]
2060 AGATCT
DpnII 222 GATC ^GATC
2061 GATC
EarI 1378 CTCTTC CTCTTC(1/4)
1531 GAAGAG
EcoO65I 636 GGTCACC G^GT[ACGT]ACC
1164 GGTGACC
HgaI 981 GACGC GACGC(5/10)
2564 GACGC
KasI 1071 GGCGCC G^GCGCC
1277 GGCGCC
Ksp632I 1378 CTCTTC CTCTTC(1/4)
1531 GAAGAG
MaeII 1760 ACGT A^CGT
2091 ACGT
MamI 804 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1683 GATTGCCATC
MboI 222 GATC ^GATC
2061 GATC
MflI 221 AGATCC [GA]^GATC[CT]
2060 AGATCT
MspA1I 34 CCGCTG C[AC]G^C[GT]G
1352 CCGCTG
NarI 1071 GGCGCC GG^CGCC
1277 GGCGCC
NdeII 222 GATC ^GATC
2061 GATC
RcaI 1763 TCATGA T^CATGA
1838 TCATGA
Sau3AI 222 GATC ^GATC
2061 GATC
SbfI 777 CCTGCAGG CCTGCA^GG
1077 CCTGCAGG
SfoI 1071 GGCGCC GGC^GCC
1277 GGCGCC
SmaI 140 CCCGGG CCC^GGG
1067 CCCGGG
SphI 928 GCATGC GCATG^C
2006 GCATGC
Sse8387I 777 CCTGCAGG CCTGCA^GG
1077 CCTGCAGG
XhoII 221 AGATCC [GA]^GATC[CT]
2060 AGATCT
XmaCI 140 CCCGGG C^CCGGG
1067 CCCGGG
XmaI 140 CCCGGG C^CCGGG
1067 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwNI Asp718I AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
MaeI MaeIII MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau96I ScrFI SfaNI
SfcI StuI TfiI TseI Tsp45I
TspRI