Restriction map for
pF1KB9392
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbvCI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsgI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrDI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRV EcoT22I FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MluI
MroI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 85 CGCG CG^CG
AflIII 53 ACGTGT A^C[GA][CT]GT
Aor51HI 2273 AGCGCT AGC^GCT
Asp700I 849 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 935 GAGCCC G[GA]GC[CT]^C
BbrPI 476 CACGTG CAC^GTG
BcnI 141 CCGGG CC^[GC]GG
BpmI 932 CTGGAG CTGGAG(16/14)
BsaAI 476 CACGTG [CT]AC^GT[GA]
BsiEI 1497 CGACCG CG[GA][CT]^CG
BsiWI 1303 CGTACG C^GTACG
BsmI 121 GAATGC GAATGC(1/-1)
Bsp1407I 2030 TGTACA T^GTACA
BspHI 2339 TCATGA T^CATGA
BsrBI 88 GAGCGG CCGCTC(-3/-3)
BsrFI 2312 GCCGGC [GA]^CCGG[CT]
BsrGI 2030 TGTACA T^GTACA
BstEII 1918 GGTTACC G^GT[ACGT]ACC
BstPI 1918 GGTTACC G^GT[ACGT]ACC
BstUI 85 CGCG CG^CG
BstXI 1419 CCATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 681 GGATCT [GA]^GATC[CT]
Cfr10I 2312 GCCGGC [GA]^CCGG[CT]
DraI 1702 TTTAAA TTT^AAA
DraII 315 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1800 CCATGG C^C[GA][CT]GG
Eco47III 2273 AGCGCT AGC^GCT
EcoNI 1732 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 315 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1918 GGTTACC G^GT[ACGT]ACC
EcoRI 1430 GAATTC G^AATTC
FauI 90 GCGGG CCCGC(4/6)
HincII 1495 GTCGAC GT[CT]^[GA]AC
HindII 1495 GTCGAC GT[CT]^[GA]AC
MfeI 2057 CAATTG C^AATTG
MflI 681 GGATCT [GA]^GATC[CT]
MslI 1420 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2057 CAATTG C^AATTG
MvnI 85 CGCG CG^CG
NaeI 2312 GCCGGC GCC^GGC
NciI 141 CCGGG CC^[GC]GG
NcoI 1800 CCATGG C^CATGG
NgoMIV 2312 GCCGGC G^CCGGC
NspI 1070 ACATGC [GA]CATG^[CT]
PmaCI 476 CACGTG CAC^GTG
PmlI 476 CACGTG CAC^GTG
PpuMI 315 AGGACCT [GA]G^G[AT]CC[CT]
PstI 2359 CTGCAG CTGCA^G
RcaI 2339 TCATGA T^CATGA
SalI 1495 GTCGAC G^TCGAC
ScaI 1255 AGTACT AGT^ACT
SspBI 2030 TGTACA T^GTACA
XbaI 663 TCTAGA T^CTAGA
XhoII 681 GGATCT [GA]^GATC[CT]
XmnI 849 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1495 GTCGAC GT^[AC][GT]AC
1895 GTCTAC
Alw44I 39 GTGCAC G^TGCAC
1565 GTGCAC
ApaLI 39 GTGCAC G^TGCAC
1565 GTGCAC
AspHI 39 GTGCAC G[AT]GC[AT]^C
1565 GTGCAC
BalI 1780 TGGCCA TGG^CCA
2325 TGGCCA
BbsI 613 GAAGAC GAAGAC(2/6)
813 GAAGAC
BciVI 5 GGATAC GTATCC(6/5)
1908 GTATCC
BclI 231 TGATCA T^GATCA
1578 TGATCA
BpuAI 613 GAAGAC GAAGAC(2/6)
813 GAAGAC
BsaI 927 GAGACC GGTCTC(1/5)
1007 GAGACC
BseRI 2078 CTCCTC GAGGAG(10/8)
76 GAGGAG
BsiHKAI 39 GTGCAC G[AT]GC[AT]^C
1565 GTGCAC
BsmFI 1141 GTCCC GGGAC(10/14)
1324 GTCCC
BssSI 692 CACGAG CACGAG(-5/-1)
344 CTCGTG
DdeI 685 CTTAG C^T[ACGT]AG
2237 CTGAG
EaeI 1780 TGGCCA [CT]^GGCC[GA]
2325 TGGCCA
FbaI 231 TGATCA T^GATCA
1578 TGATCA
HapII 141 CCGG C^CGG
2313 CCGG
HpaII 141 CCGG C^CGG
2313 CCGG
MluNI 1780 TGGCCA TGG^CCA
2325 TGGCCA
MscI 1780 TGGCCA TGG^CCA
2325 TGGCCA
MspA1I 208 CAGCTG C[AC]G^C[GT]G
1574 CAGCTG
MspI 141 CCGG C^CGG
2313 CCGG
PvuII 208 CAGCTG CAG^CTG
1574 CAGCTG
SmlI 822 CTCAAG C^T[CT][GA]AG
1216 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I