Restriction map for
pF1KB9351
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# NO CUT ENZYMES ( 68 enzymes) #
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Acc65I AclI AocI ApaI AscI
Asp718I AvrII BbeI BbrPI BciVI
BclI BglI BlnI BsaAI BsaBI
BsiEI BsiWI BsmBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI EagI EclXI Eco52I Eco81I
EcoO65I EcoRV FbaI FseI HgaI
HpaI KasI KpnI MamI MluI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 2936 GACGTC GACGT^C
AccII 4839 CGCG CG^CG
AccIII 812 TCCGGA T^CCGGA
AcyI 2936 GACGTC G[GA]^CG[CT]C
AflII 2317 CTTAAG C^TTAAG
AgeI 4796 ACCGGT A^CCGGT
Aor51HI 574 AGCGCT AGC^GCT
AseI 3075 ATTAAT AT^TAAT
AsnI 3075 ATTAAT AT^TAAT
AspI 1557 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5189 CCCGGG C^[CT]CG[GA]G
AviII 3129 TGCGCA TGC^GCA
BamHI 175 GGATCC G^GATCC
BbvCI 5613 CCTCAGC CCTCAGC(-5/-2)
BfrI 2317 CTTAAG C^TTAAG
BsaHI 2936 GACGTC G[GA]^CG[CT]C
BseAI 812 TCCGGA T^CCGGA
BsoBI 5189 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2636 TGTACA T^GTACA
BspDI 3373 ATCGAT AT^CGAT
BspEI 812 TCCGGA T^CCGGA
BspLU11I 4380 ACATGT A^CATGT
BsrFI 4796 ACCGGT [GA]^CCGG[CT]
BsrGI 2636 TGTACA T^GTACA
BstUI 4839 CGCG CG^CG
Cfr10I 4796 ACCGGT [GA]^CCGG[CT]
ClaI 3373 ATCGAT AT^CGAT
DrdI 4488 GACTTATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 574 AGCGCT AGC^GCT
EcoRI 5527 GAATTC G^AATTC
FspI 3129 TGCGCA TGC^GCA
Hin1I 2936 GACGTC G[GA]^CG[CT]C
KspI 4838 CCGCGG CCGC^GG
MroI 812 TCCGGA T^CCGGA
MvnI 4839 CGCG CG^CG
NdeI 450 CATATG CA^TATG
PflFI 1557 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4796 ACCGGT A^CCGGT
PshBI 3075 ATTAAT AT^TAAT
SacI 3166 GAGCTC GAGCT^C
SacII 4838 CCGCGG CCGC^GG
SexAI 1487 ACCTGGT A^CC[AT]GGT
SmaI 5189 CCCGGG CCC^GGG
SspBI 2636 TGTACA T^GTACA
Tth111I 1557 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 5189 CCCGGG C^CCGGG
XmaI 5189 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflIII 1858 ACGTGT A^C[GA][CT]GT
4380 ACATGT
AhdI 2612 GACTTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3868 GACAGAGAGTC
AspEI 2612 GACTTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3868 GACAGAGAGTC
BbsI 5393 GAAGAC GAAGAC(2/6)
147 GTCTTC
BcgI 73 CGAGACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4608 GCAGCTTTGTCG
BglII 2176 AGATCT A^GATCT
4141 AGATCT
BlpI 2021 GCTTAGC GC^T[ACGT]AGC
5643 GCTGAGC
Bpu1102I 2021 GCTTAGC GC^T[ACGT]AGC
5643 GCTGAGC
BpuAI 5393 GAAGAC GAAGAC(2/6)
147 GTCTTC
BsaI 74 GAGACC GGTCTC(1/5)
5344 GAGACC
BsaWI 812 TCCGGA [AT]^CCGG[AT]
4796 ACCGGT
BspHI 1698 TCATGA T^CATGA
1991 TCATGA
BspMI 2277 ACCTGC ACCTGC(4/8)
5696 ACCTGC
BsrBI 4745 CCGCTC CCGCTC(-3/-3)
5482 GAGCGG
CelII 2021 GCTTAGC GC^T[ACGT]AGC
5643 GCTGAGC
DraI 846 TTTAAA TTT^AAA
4986 TTTAAA
Eam1105I 2612 GACTTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3868 GACAGAGAGTC
HindIII 2201 AAGCTT A^AGCTT
5727 AAGCTT
PpuMI 1947 AGGACCC [GA]G^G[AT]CC[CT]
4521 AGGTCCT
PvuII 121 CAGCTG CAG^CTG
5641 CAGCTG
RcaI 1698 TCATGA T^CATGA
1991 TCATGA
SmlI 2317 CTTAAG C^T[CT][GA]AG
2622 CTTGAG
SspI 2123 AATATT AAT^ATT
5255 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI