Restriction map for
pF1KB9239
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AvrII BbeI BglI
BlnI BseAI BsiWI BsmI Bsp1407I
BspEI BspLU11I BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I CpoI DraIII
DrdI Eco47III EcoO65I EcoRI FseI
KasI KpnI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI PacI PinAI PmeI PshBI
PvuI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SnaBI
SpeI SphI Sse8387I SspBI SwaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 672 AACGTT AA^CGTT
AflII 418 CTTAAG C^TTAAG
AhdI 761 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3021 CCTCAGG CC^T[ACGT]AGG
Asp700I 3079 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 761 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3386 TGCGCA TGC^GCA
BbrPI 1348 CACGTG CAC^GTG
BclI 3317 TGATCA T^GATCA
BfrI 418 CTTAAG C^TTAAG
BglII 1941 AGATCT A^GATCT
BlpI 3034 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3034 GCTCAGC GC^T[ACGT]AGC
BsaWI 1877 TCCGGT [AT]^CCGG[AT]
BspDI 1213 ATCGAT AT^CGAT
BstAPI 3842 GCATCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3806 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3021 CCTCAGG CC^T[ACGT]AGG
CelII 3034 GCTCAGC GC^T[ACGT]AGC
ClaI 1213 ATCGAT AT^CGAT
DraI 520 TTTAAA TTT^AAA
EagI 2360 CGGCCG C^GGCCG
Eam1105I 761 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2360 CGGCCG C^GGCCG
Eco52I 2360 CGGCCG C^GGCCG
Eco81I 3021 CCTCAGG CC^T[ACGT]AGG
EcoRV 933 GATATC GAT^ATC
EcoT22I 3902 ATGCAT ATGCA^T
FbaI 3317 TGATCA T^GATCA
FspI 3386 TGCGCA TGC^GCA
HindIII 3831 AAGCTT A^AGCTT
HpaI 1114 GTTAAC GTT^AAC
KspI 3594 CCGCGG CCGC^GG
NsiI 3902 ATGCAT ATGCA^T
PmaCI 1348 CACGTG CAC^GTG
PmlI 1348 CACGTG CAC^GTG
PshAI 258 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 672 AACGTT AA^CGTT
SacII 3594 CCGCGG CCGC^GG
SapI 2527 GAAGAGC GCTCTTC(1/4)
SgrAI 42 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3275 CCCGGG CCC^GGG
SspI 1171 AATATT AAT^ATT
Tsp45I 3860 GTCAC ^GT[GC]AC
XbaI 2523 TCTAGA T^CTAGA
XmaCI 3275 CCCGGG C^CCGGG
XmaI 3275 CCCGGG C^CCGGG
XmnI 3079 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 1246 GTCTAC GT^[AC][GT]AC
3868 GTCTAC
BbvCI 1689 CCTCAGC CCTCAGC(-5/-2)
2133 GCTGAGG
BcgI 1605 CGACCTTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2285 GCATGATGTTCG
BsaAI 1348 CACGTG [CT]AC^GT[GA]
1969 CACGTA
BsaBI 468 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3060 GATCAACATC
BsaI 3154 GAGACC GGTCTC(1/5)
1978 GGTCTC
BsiEI 2360 CGGCCG CG[GA][CT]^CG
2686 CGACCG
BsmBI 1534 GAGACG CGTCTC(1/5)
3604 GAGACG
BspHI 2672 TCATGA T^CATGA
3746 TCATGA
BsrBI 3339 CCGCTC CCGCTC(-3/-3)
184 GAGCGG
BstBI 1994 TTCGAA TT^CGAA
2831 TTCGAA
MamI 468 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3060 GATCAACATC
NaeI 155 GCCGGC GCC^GGC
1498 GCCGGC
NgoMIV 155 GCCGGC G^CCGGC
1498 GCCGGC
NspI 1750 GCATGT [GA]CATG^[CT]
1766 ACATGC
NspV 1994 TTCGAA TT^CGAA
2831 TTCGAA
PaeR7I 1206 CTCGAG C^TCGAG
4236 CTCGAG
PflMI 646 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3207 CCAGCGCCTGG
PpuMI 3017 AGGACCT [GA]G^G[AT]CC[CT]
3278 GGGACCT
RcaI 2672 TCATGA T^CATGA
3746 TCATGA
SfuI 1994 TTCGAA TT^CGAA
2831 TTCGAA
Van91I 646 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3207 CCAGCGCCTGG
XcmI 2309 CCAAAGGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3881 CCAGCCTCGGCATGG
XhoI 1206 CTCGAG C^TCGAG
4236 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflFI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI Tth111I TthHB8I
XhoII