Restriction map for
pF1KB9075
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BfrI BglI BlnI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrDI BstBI
ClaI CpoI EagI EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI Hin1I KasI KpnI KspI
MamI MluI MroI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 1583 GAATTT [GA]^AATT[CT]
AflIII 3242 ACGTGT A^C[GA][CT]GT
AhdI 3241 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3767 AGCGCT AGC^GCT
ApoI 1583 GAATTT [GA]^AATT[CT]
Asp700I 3765 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3241 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1373 GTATCC GTATCC(6/5)
BglII 2991 AGATCT A^GATCT
BsaAI 2559 TACGTG [CT]AC^GT[GA]
BseRI 2947 GAGGAG GAGGAG(10/8)
BsgI 2691 CTGCAC GTGCAG(16/14)
BsmBI 1754 GAGACG CGTCTC(1/5)
BsrBI 2431 GAGCGG CCGCTC(-3/-3)
BsrFI 1058 ACCGGC [GA]^CCGG[CT]
BssHII 35 GCGCGC G^CGCGC
Bst1107I 1921 GTATAC GTA^TAC
BstAPI 120 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1118 GGTTACC G^GT[ACGT]ACC
BstPI 1118 GGTTACC G^GT[ACGT]ACC
BstZ17I 1921 GTATAC GTA^TAC
Cfr10I 1058 ACCGGC [GA]^CCGG[CT]
DraIII 2008 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 16 GACACCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2600 CCACGG C^C[GA][CT]GG
Eam1105I 3241 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3767 AGCGCT AGC^GCT
EcoO65I 1118 GGTTACC G^GT[ACGT]ACC
FauI 3966 CCCGC CCCGC(4/6)
HaeII 3767 AGCGCT [GA]GCGC^[CT]
HgaI 2876 GCGTC GACGC(5/10)
MfeI 750 CAATTG C^AATTG
MunI 750 CAATTG C^AATTG
NdeI 1367 CATATG CA^TATG
NheI 1555 GCTAGC G^CTAGC
NspI 2287 ACATGC [GA]CATG^[CT]
PflMI 2450 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Van91I 2450 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1579 TCTAGA T^CTAGA
XcmI 3879 CCATTCCAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3765 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 395 GTAGAC GT^[AC][GT]AC
1921 GTATAC
AccII 36 CGCG CG^CG
3954 CGCG
AocI 3281 CCTTAGG CC^T[ACGT]AGG
3336 CCTGAGG
BbsI 2355 GTCTTC GAAGAC(2/6)
3039 GTCTTC
BcgI 2889 CGAAAGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1081 GCATTTCCCTCG
BclI 2004 TGATCA T^GATCA
2276 TGATCA
BpuAI 2355 GTCTTC GAAGAC(2/6)
3039 GTCTTC
BsaI 3416 GAGACC GGTCTC(1/5)
3290 GGTCTC
Bsp1407I 413 TGTACA T^GTACA
3483 TGTACA
BspHI 1868 TCATGA T^CATGA
3656 TCATGA
BsrGI 413 TGTACA T^GTACA
3483 TGTACA
BssSI 777 CACGAG CACGAG(-5/-1)
1154 CTCGTG
BstUI 36 CGCG CG^CG
3954 CGCG
BstXI 2978 CCATCACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3020 CCAGAGGCATGG
Bsu36I 3281 CCTTAGG CC^T[ACGT]AGG
3336 CCTGAGG
Eco81I 3281 CCTTAGG CC^T[ACGT]AGG
3336 CCTGAGG
EcoT14I 1562 CCTTGG C^C[AT][AT]GG
3794 CCAAGG
FbaI 2004 TGATCA T^GATCA
2276 TGATCA
HindIII 1644 AAGCTT A^AGCTT
2892 AAGCTT
MvnI 36 CGCG CG^CG
3954 CGCG
PstI 264 CTGCAG CTGCA^G
1905 CTGCAG
RcaI 1868 TCATGA T^CATGA
3656 TCATGA
ScaI 268 AGTACT AGT^ACT
3368 AGTACT
SmaI 2468 CCCGGG CCC^GGG
3131 CCCGGG
SspBI 413 TGTACA T^GTACA
3483 TGTACA
StuI 2841 AGGCCT AGG^CCT
3797 AGGCCT
StyI 1562 CCTTGG C^C[AT][AT]GG
3794 CCAAGG
XmaCI 2468 CCCGGG C^CCGGG
3131 CCCGGG
XmaI 2468 CCCGGG C^CCGGG
3131 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII