Restriction map for
pF1KB7021
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI AspEI
AspI AvrII BbrPI BbsI BciVI
BclI BfrI BglI BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1357 GGTACC G^GTACC
Alw44I 841 GTGCAC G^TGCAC
Aor51HI 85 AGCGCT AGC^GCT
ApaI 24 GGGCCC GGGCC^C
ApaLI 841 GTGCAC G^TGCAC
Asp700I 1083 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1357 GGTACC G^GTACC
AviII 977 TGCGCA TGC^GCA
BalI 428 TGGCCA TGG^CCA
BamHI 924 GGATCC G^GATCC
BbeI 1054 GGCGCC GGCGC^C
BbvCI 1314 CCTCAGC CCTCAGC(-5/-2)
BcgI 1530 GCAGGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 994 CTAG C^TAG
BglII 1472 AGATCT A^GATCT
BlpI 706 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 706 GCTGAGC GC^T[ACGT]AGC
BsaAI 877 TACGTG [CT]AC^GT[GA]
BseRI 1535 CTCCTC GAGGAG(10/8)
BsiEI 1293 CGGCCG CG[GA][CT]^CG
BsmI 216 GAATGC GAATGC(1/-1)
BsrBI 796 GAGCGG CCGCTC(-3/-3)
BsrFI 1291 GCCGGC [GA]^CCGG[CT]
BstEII 1248 GGTGACC G^GT[ACGT]ACC
BstPI 1248 GGTGACC G^GT[ACGT]ACC
BstXI 891 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 706 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1291 GCCGGC [GA]^CCGG[CT]
EagI 1293 CGGCCG C^GGCCG
EarI 349 CTCTTC CTCTTC(1/4)
EclXI 1293 CGGCCG C^GGCCG
Eco47III 85 AGCGCT AGC^GCT
Eco52I 1293 CGGCCG C^GGCCG
EcoO65I 1248 GGTGACC G^GT[ACGT]ACC
EcoT14I 1046 CCAAGG C^C[AT][AT]GG
FspI 977 TGCGCA TGC^GCA
KasI 1054 GGCGCC G^GCGCC
KpnI 1357 GGTACC GGTAC^C
Ksp632I 349 CTCTTC CTCTTC(1/4)
MaeI 994 CTAG C^TAG
MaeII 878 ACGT A^CGT
MluNI 428 TGGCCA TGG^CCA
MscI 428 TGGCCA TGG^CCA
NaeI 1291 GCCGGC GCC^GGC
NarI 1054 GGCGCC GG^CGCC
NgoMIV 1291 GCCGGC G^CCGGC
NspI 1553 ACATGC [GA]CATG^[CT]
PpuMI 626 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 401 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 1054 GGCGCC GGC^GCC
SmlI 403 CTCAAG C^T[CT][GA]AG
StyI 1046 CCAAGG C^C[AT][AT]GG
TfiI 821 GAATC G^A[AT]TC
XcmI 563 CCAACACCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1083 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 613 GAGCAC G[AT]GC[AT]^C
841 GTGCAC
AvaII 495 GGACC G^G[AT]CC
627 GGACC
BpmI 723 CTCCAG CTGGAG(16/14)
1135 CTGGAG
BsiHKAI 613 GAGCAC G[AT]GC[AT]^C
841 GTGCAC
DraIII 616 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1621 CACATTGTG
MboII 350 TCTTC GAAGA(8/7)
464 TCTTC
NlaIII 72 CATG CATG^
1554 CATG
PflMI 678 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1515 CCAGGAGCTGG
SmaI 27 CCCGGG CCC^GGG
1391 CCCGGG
Van91I 678 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1515 CCAGGAGCTGG
XmaCI 27 CCCGGG C^CCGGG
1391 CCCGGG
XmaI 27 CCCGGG C^CCGGG
1391 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I XhoII