Restriction map for
pF1KB7019
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsiEI BsiWI
BsmBI BspDI BspMI BsrFI BssHII
BssSI BstAPI BstEII BstPI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI Hin1I
HpaI HphI KasI KpnI Ksp632I
KspI MfeI MluI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1284 GTATAC GT^[AC][GT]AC
AccII 1580 CGCG CG^CG
AccIII 8 TCCGGA T^CCGGA
AflIII 678 ACATGT A^C[GA][CT]GT
AhdI 2014 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 581 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1072 GGGCCC GGGCC^C
AseI 171 ATTAAT AT^TAAT
AsnI 171 ATTAAT AT^TAAT
AspEI 2014 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 945 CCCGAG C^[CT]CG[GA]G
BfaI 958 CTAG C^TAG
BsaBI 178 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 8 TCCGGA T^CCGGA
BsoBI 945 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1220 TGTACA T^GTACA
BspEI 8 TCCGGA T^CCGGA
BspHI 458 TCATGA T^CATGA
BspLU11I 678 ACATGT A^CATGT
BsrBI 1890 CCGCTC CCGCTC(-3/-3)
BsrDI 1929 CATTGC GCAATG(2/0)
BsrGI 1220 TGTACA T^GTACA
Bst1107I 1284 GTATAC GTA^TAC
BstBI 596 TTCGAA TT^CGAA
BstUI 1580 CGCG CG^CG
BstZ17I 1284 GTATAC GTA^TAC
DsaI 2026 CCGTGG C^C[GA][CT]GG
Eam1105I 2014 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1106 GAATTC G^AATTC
FauI 1581 GCGGG CCCGC(4/6)
HaeII 397 GGCGCT [GA]GCGC^[CT]
HgaI 491 GACGC GACGC(5/10)
HincII 829 GTCAAC GT[CT]^[GA]AC
HindII 829 GTCAAC GT[CT]^[GA]AC
HindIII 254 AAGCTT A^AGCTT
MaeI 958 CTAG C^TAG
MaeII 512 ACGT A^CGT
MamI 178 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 8 TCCGGA T^CCGGA
MvnI 1580 CGCG CG^CG
MwoI 2142 GCAACATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 678 ACATGT [GA]CATG^[CT]
NspV 596 TTCGAA TT^CGAA
PpuMI 1583 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 171 ATTAAT AT^TAAT
RcaI 458 TCATGA T^CATGA
SacI 1411 GAGCTC GAGCT^C
SfuI 596 TTCGAA TT^CGAA
SpeI 957 ACTAGT A^CTAGT
SspBI 1220 TGTACA T^GTACA
SspI 232 AATATT AAT^ATT
StuI 1249 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 1106 GAATTC [GA]^AATT[CT]
1469 AAATTT
ApoI 1106 GAATTC [GA]^AATT[CT]
1469 AAATTT
BalI 271 TGGCCA TGG^CCA
1605 TGGCCA
BanII 1072 GGGCCC G[GA]GC[CT]^C
1411 GAGCTC
BbsI 883 GAAGAC GAAGAC(2/6)
1652 GAAGAC
BclI 533 TGATCA T^GATCA
630 TGATCA
BpmI 1420 CTGGAG CTGGAG(16/14)
1768 CTGGAG
BpuAI 883 GAAGAC GAAGAC(2/6)
1652 GAAGAC
BsaWI 8 TCCGGA [AT]^CCGG[AT]
191 ACCGGA
BsgI 1531 CTGCAC GTGCAG(16/14)
1735 GTGCAG
BsmAI 1665 GAGAC GTCTC(1/5)
1785 GTCTC
BsrI 808 ACTGG ACTGG(1/-1)
1633 ACTGG
BstXI 1542 CCACATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1760 CCACTTTTCTGG
BstYI 61 GGATCT [GA]^GATC[CT]
318 AGATCC
EaeI 271 TGGCCA [CT]^GGCC[GA]
1605 TGGCCA
EcoT14I 1169 CCAAGG C^C[AT][AT]GG
1911 CCAAGG
FbaI 533 TGATCA T^GATCA
630 TGATCA
HapII 9 CCGG C^CGG
192 CCGG
HpaII 9 CCGG C^CGG
192 CCGG
MflI 61 GGATCT [GA]^GATC[CT]
318 AGATCC
MluNI 271 TGGCCA TGG^CCA
1605 TGGCCA
MscI 271 TGGCCA TGG^CCA
1605 TGGCCA
MspI 9 CCGG C^CGG
192 CCGG
PvuII 1084 CAGCTG CAG^CTG
1724 CAGCTG
SfcI 159 CTACAG C^T[GA][CT]AG
1934 CTACAG
StyI 1169 CCAAGG C^C[AT][AT]GG
1911 CCAAGG
Tsp45I 1192 GTCAC ^GT[GC]AC
2008 GTGAC
XhoII 61 GGATCT [GA]^GATC[CT]
318 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I