Restriction map for
pF1KB7018
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AgeI AocI Aor51HI AscI
Asp700I Asp718I AvaI AviII AvrII
BalI BamHI BbvCI BcgI BciVI
BglI BlnI BsaI BsiWI BsoBI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII BstBI BstEII BstPI BstXI
Bsu36I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MfeI MluI
MluNI MscI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PinAI PmeI
PpuMI PshAI PvuI PvuII RsrII
SacI SacII SalI SbfI SfiI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI StuI SwaI XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 2648 TCCGGA T^CCGGA
AclI 819 AACGTT AA^CGTT
AflII 306 CTTAAG C^TTAAG
AhdI 2539 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1978 GGGCCC GGGCC^C
AseI 937 ATTAAT AT^TAAT
AsnI 937 ATTAAT AT^TAAT
AspEI 2539 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2908 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 16 GGCGCC GGCGC^C
BbrPI 285 CACGTG CAC^GTG
BcnI 181 CCGGG CC^[GC]GG
BfrI 306 CTTAAG C^TTAAG
BglII 2206 AGATCT A^GATCT
BsaBI 2710 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2648 TCCGGA [AT]^CCGG[AT]
BseAI 2648 TCCGGA T^CCGGA
BsiEI 2609 CGGTCG CG[GA][CT]^CG
BsmBI 101 CGTCTC CGTCTC(1/5)
BsmFI 2182 GTCCC GGGAC(10/14)
BspEI 2648 TCCGGA T^CCGGA
BspHI 509 TCATGA T^CATGA
BspMI 2673 ACCTGC ACCTGC(4/8)
BsrBI 363 CCGCTC CCGCTC(-3/-3)
BsrI 1366 CCAGT ACTGG(1/-1)
BssSI 1261 CTCGTG CACGAG(-5/-1)
Bst1107I 2521 GTATAC GTA^TAC
BstZ17I 2521 GTATAC GTA^TAC
DraI 1522 TTTAAA TTT^AAA
DraII 1977 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 2834 CCGTGG C^C[GA][CT]GG
EaeI 1835 CGGCCA [CT]^GGCC[GA]
Eam1105I 2539 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1977 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 682 GAATTC G^AATTC
HgaI 56 GCGTC GACGC(5/10)
HindIII 346 AAGCTT A^AGCTT
KasI 16 GGCGCC G^GCGCC
MamI 2710 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2648 TCCGGA T^CCGGA
MspA1I 402 CCGCTG C[AC]G^C[GT]G
NarI 16 GGCGCC GG^CGCC
NciI 181 CCGGG CC^[GC]GG
NspI 1271 GCATGC [GA]CATG^[CT]
PacI 1819 TTAATTAA TTAAT^TAA
PflFI 2908 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 285 CACGTG CAC^GTG
PmlI 285 CACGTG CAC^GTG
PshBI 937 ATTAAT AT^TAAT
Psp1406I 819 AACGTT AA^CGTT
RcaI 509 TCATGA T^CATGA
SapI 68 GCTCTTC GCTCTTC(1/4)
ScaI 2137 AGTACT AGT^ACT
SexAI 2309 ACCAGGT A^CC[AT]GGT
SfoI 16 GGCGCC GGC^GCC
SnaBI 1117 TACGTA TAC^GTA
SphI 1271 GCATGC GCATG^C
Tth111I 2908 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1192 GACGTC GACGT^C
2539 GACGTC
AccI 2521 GTATAC GT^[AC][GT]AC
2839 GTAGAC
AflIII 284 ACACGT A^C[GA][CT]GT
2525 ACACGT
BanII 1303 GAGCCC G[GA]GC[CT]^C
1978 GGGCCC
BlpI 2295 GCTGAGC GC^T[ACGT]AGC
2655 GCTCAGC
Bpu1102I 2295 GCTGAGC GC^T[ACGT]AGC
2655 GCTCAGC
BsaAI 285 CACGTG [CT]AC^GT[GA]
1117 TACGTA
BseRI 2883 CTCCTC GAGGAG(10/8)
1669 GAGGAG
BsrFI 274 ACCGGC [GA]^CCGG[CT]
2607 GCCGGT
BstAPI 1589 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2080 GCAGAAGCTGC
CelII 2295 GCTGAGC GC^T[ACGT]AGC
2655 GCTCAGC
Cfr10I 274 ACCGGC [GA]^CCGG[CT]
2607 GCCGGT
DrdI 133 GACTTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
391 GACACGCTGGTC
EarI 69 CTCTTC CTCTTC(1/4)
464 CTCTTC
FauI 2248 CCCGC CCCGC(4/6)
502 GCGGG
HaeII 7 GGCGCT [GA]GCGC^[CT]
16 GGCGCC
HincII 358 GTTGAC GT[CT]^[GA]AC
822 GTTGAC
HindII 358 GTTGAC GT[CT]^[GA]AC
822 GTTGAC
HphI 793 GGTGA GGTGA(8/7)
2014 GGTGA
Ksp632I 69 CTCTTC CTCTTC(1/4)
464 CTCTTC
PflMI 1278 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1399 CCACCGTTTGG
PstI 2127 CTGCAG CTGCA^G
2811 CTGCAG
TaqI 2475 TCGA T^CGA
2612 TCGA
TthHB8I 2475 TCGA T^CGA
2612 TCGA
Van91I 1278 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1399 CCACCGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaII BanI BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII