Restriction map for
pF1KB7017
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvrII BalI BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
SacI SalI SbfI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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Acc65I 296 GGTACC G^GTACC
AccI 721 GTCTAC GT^[AC][GT]AC
Asp718I 296 GGTACC G^GTACC
BamHI 1250 GGATCC G^GATCC
BfaI 598 CTAG C^TAG
BglII 319 AGATCT A^GATCT
BsaI 1435 GAGACC GGTCTC(1/5)
BsgI 1731 CTGCAC GTGCAG(16/14)
BsiWI 524 CGTACG C^GTACG
BspMI 1790 ACCTGC ACCTGC(4/8)
CpoI 2384 CGGACCG CG^G[AT]CCG
FseI 946 GGCCGGCC GGCCGG^CC
HincII 217 GTCAAC GT[CT]^[GA]AC
HindII 217 GTCAAC GT[CT]^[GA]AC
KpnI 296 GGTACC GGTAC^C
MaeI 598 CTAG C^TAG
MseI 755 TTAA T^TAA
PaeR7I 1283 CTCGAG C^TCGAG
PshAI 1326 GACCGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2384 CGGACCG CG^G[AT]CCG
SapI 570 GCTCTTC GCTCTTC(1/4)
ScaI 1583 AGTACT AGT^ACT
Tru9I 755 TTAA T^TAA
Tsp45I 922 GTCAC ^GT[GC]AC
XcmI 2411 CCACGGGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1283 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AatII 846 GACGTC GACGT^C
1401 GACGTC
AlwNI 578 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
1486 CAGCGGCTG
AviII 647 TGCGCA TGC^GCA
1132 TGCGCA
BbsI 1333 GTCTTC GAAGAC(2/6)
1417 GTCTTC
BbvCI 1662 CCTCAGC CCTCAGC(-5/-2)
2432 CCTCAGC
BpuAI 1333 GTCTTC GAAGAC(2/6)
1417 GTCTTC
BsrBI 2362 CCGCTC CCGCTC(-3/-3)
1876 GAGCGG
BsrI 57 ACTGG ACTGG(1/-1)
2496 ACTGG
BstAPI 175 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1485 GCAGCGGCTGC
BstYI 319 AGATCT [GA]^GATC[CT]
1250 GGATCC
EarI 553 CTCTTC CTCTTC(1/4)
571 CTCTTC
EcoT14I 2069 CCAAGG C^C[AT][AT]GG
2420 CCTTGG
FspI 647 TGCGCA TGC^GCA
1132 TGCGCA
HphI 1785 GGTGA GGTGA(8/7)
2366 TCACC
Ksp632I 553 CTCTTC CTCTTC(1/4)
571 CTCTTC
KspI 2452 CCGCGG CCGC^GG
2580 CCGCGG
MflI 319 AGATCT [GA]^GATC[CT]
1250 GGATCC
PpuMI 1619 AGGACCC [GA]G^G[AT]CC[CT]
1980 GGGTCCC
PvuII 1034 CAGCTG CAG^CTG
1276 CAGCTG
SacII 2452 CCGCGG CCGC^GG
2580 CCGCGG
SgrAI 1088 CGCCGGTG C[GA]^CCGG[CT]G
2389 CGCCGGCG
SmlI 1283 CTCGAG C^T[CT][GA]AG
1363 CTCAAG
StyI 2069 CCAAGG C^C[AT][AT]GG
2420 CCTTGG
XhoII 319 AGATCT [GA]^GATC[CT]
1250 GGATCC
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AocI ApaI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI TaqI TseI TspRI
TthHB8I Van91I XmaCI XmaI