Restriction map for
pF1KB7008
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# NO CUT ENZYMES ( 124 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI Ksp632I MaeIII MamI MfeI
MluI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfoI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 328 GACGTC GACGT^C
AccIII 332 TCCGGA T^CCGGA
AfaI 126 GTAC GT^AC
Alw44I 369 GTGCAC G^TGCAC
AlwNI 625 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 369 GTGCAC G^TGCAC
Asp700I 1323 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 894 GTATCC GTATCC(6/5)
BfaI 304 CTAG C^TAG
BsaI 538 GGTCTC GGTCTC(1/5)
BseAI 332 TCCGGA T^CCGGA
BspEI 332 TCCGGA T^CCGGA
BsrFI 1385 ACCGGC [GA]^CCGG[CT]
BstAPI 401 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 79 TTCGAA TT^CGAA
Cfr10I 1385 ACCGGC [GA]^CCGG[CT]
KspI 932 CCGCGG CCGC^GG
MaeI 304 CTAG C^TAG
MroI 332 TCCGGA T^CCGGA
MspA1I 932 CCGCGG C[AC]G^C[GT]G
NspV 79 TTCGAA TT^CGAA
PleI 1177 GACTC GAGTC(4/5)
PpuMI 62 AGGACCT [GA]G^G[AT]CC[CT]
PstI 952 CTGCAG CTGCA^G
RsaI 126 GTAC GT^AC
SacII 932 CCGCGG CCGC^GG
SfiI 441 GGCCCAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 79 TTCGAA TT^CGAA
SmaI 170 CCCGGG CCC^GGG
SphI 548 GCATGC GCATG^C
StuI 959 AGGCCT AGG^CCT
TfiI 175 GATTC G^A[AT]TC
XmaCI 170 CCCGGG C^CCGGG
XmaI 170 CCCGGG C^CCGGG
XmnI 1323 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 933 CGCG CG^CG
966 CGCG
AcyI 328 GACGTC G[GA]^CG[CT]C
832 GGCGTC
AocI 767 CCTCAGG CC^T[ACGT]AGG
1372 CCTAAGG
AspHI 369 GTGCAC G[AT]GC[AT]^C
376 GTGCTC
AvaI 42 CCCGAG C^[CT]CG[GA]G
170 CCCGGG
BanII 346 GAGCCC G[GA]GC[CT]^C
927 GAGCCC
BbsI 213 GAAGAC GAAGAC(2/6)
277 GAAGAC
BbvCI 675 CCTCAGC CCTCAGC(-5/-2)
436 GCTGAGG
BpuAI 213 GAAGAC GAAGAC(2/6)
277 GAAGAC
BsaHI 328 GACGTC G[GA]^CG[CT]C
832 GGCGTC
BsaWI 281 ACCGGA [AT]^CCGG[AT]
332 TCCGGA
BseRI 55 GAGGAG GAGGAG(10/8)
272 GAGGAG
BsiHKAI 369 GTGCAC G[AT]GC[AT]^C
376 GTGCTC
BsmAI 539 GTCTC GTCTC(1/5)
808 GTCTC
BsmFI 355 GGGAC GGGAC(10/14)
1201 GTCCC
BsoBI 42 CCCGAG C^[CT]CG[GA]G
170 CCCGGG
BstUI 933 CGCG CG^CG
966 CGCG
Bsu36I 767 CCTCAGG CC^T[ACGT]AGG
1372 CCTAAGG
Eco81I 767 CCTCAGG CC^T[ACGT]AGG
1372 CCTAAGG
EcoNI 951 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1158 CCTGCCCCAGG
EcoT14I 683 CCATGG C^C[AT][AT]GG
692 CCATGG
HgaI 216 GACGC GACGC(5/10)
833 GCGTC
Hin1I 328 GACGTC G[GA]^CG[CT]C
832 GGCGTC
HincII 835 GTCAAC GT[CT]^[GA]AC
1237 GTTGAC
HindII 835 GTCAAC GT[CT]^[GA]AC
1237 GTTGAC
HinfI 175 GATTC G^A[ACGT]TC
1177 GACTC
MaeII 329 ACGT A^CGT
1214 ACGT
MvnI 933 CGCG CG^CG
966 CGCG
NcoI 683 CCATGG C^CATGG
692 CCATGG
NspI 548 GCATGC [GA]CATG^[CT]
575 GCATGT
StyI 683 CCATGG C^C[AT][AT]GG
692 CCATGG
XcmI 683 CCATGGCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1314 CCAGGCCATGAATGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BanI BbvI BcnI BglI BmyI
BpmI BsaJI BsgI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MflI MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI TspRI
TthHB8I XhoII