Restriction map for
pF1KB6007
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbvCI BciVI BfaI
BfrI BglI BglII BlnI BsiWI
BspDI BsrFI BssHII BssSI BstBI
Cfr10I ClaI CpoI DraI DraIII
DsaI Eam1105I EcoRV FseI FspI
HindIII HpaI KpnI KspI MaeI
MfeI MluI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 257 TCCGGA T^CCGGA
Alw44I 379 GTGCAC G^TGCAC
AocI 1449 CCTGAGG CC^T[ACGT]AGG
ApaI 1757 GGGCCC GGGCC^C
ApaLI 379 GTGCAC G^TGCAC
Asp700I 1662 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1303 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 284 TGGCCA TGG^CCA
BamHI 740 GGATCC G^GATCC
BbeI 2100 GGCGCC GGCGC^C
BcgI 1868 GCATTCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1748 TGATCA T^GATCA
BlpI 396 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC
BsaBI 45 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 257 TCCGGA [AT]^CCGG[AT]
BseAI 257 TCCGGA T^CCGGA
BspEI 257 TCCGGA T^CCGGA
BspHI 1745 TCATGA T^CATGA
BspLU11I 1931 ACATGT A^CATGT
BsrBI 2076 GAGCGG CCGCTC(-3/-3)
BsrDI 486 GCAATG GCAATG(2/0)
Bst1107I 862 GTATAC GTA^TAC
BstAPI 1167 GCATTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 603 GGTGACC G^GT[ACGT]ACC
BstPI 603 GGTGACC G^GT[ACGT]ACC
BstZ17I 862 GTATAC GTA^TAC
Bsu36I 1449 CCTGAGG CC^T[ACGT]AGG
CelII 396 GCTGAGC GC^T[ACGT]AGC
EagI 1001 CGGCCG C^GGCCG
EclXI 1001 CGGCCG C^GGCCG
Eco52I 1001 CGGCCG C^GGCCG
Eco81I 1449 CCTGAGG CC^T[ACGT]AGG
EcoO65I 603 GGTGACC G^GT[ACGT]ACC
EcoT22I 1165 ATGCAT ATGCA^T
FbaI 1748 TGATCA T^GATCA
HincII 682 GTTGAC GT[CT]^[GA]AC
HindII 682 GTTGAC GT[CT]^[GA]AC
KasI 2100 GGCGCC G^GCGCC
MamI 45 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 284 TGGCCA TGG^CCA
MroI 257 TCCGGA T^CCGGA
MscI 284 TGGCCA TGG^CCA
NarI 2100 GGCGCC GG^CGCC
NsiI 1165 ATGCAT ATGCA^T
PflFI 1303 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2298 GACGTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1745 TCATGA T^CATGA
SapI 1020 GCTCTTC GCTCTTC(1/4)
SexAI 328 ACCTGGT A^CC[AT]GGT
SfoI 2100 GGCGCC GGC^GCC
StuI 2246 AGGCCT AGG^CCT
TfiI 265 GATTC G^A[AT]TC
Tth111I 1303 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1662 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1414 GACGTC GACGT^C
1521 GACGTC
AccI 862 GTATAC GT^[AC][GT]AC
2053 GTCTAC
AccII 1071 CGCG CG^CG
1306 CGCG
AflIII 878 ACGTGT A^C[GA][CT]GT
1931 ACATGT
AlwNI 413 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
950 CAGCGTCTG
Aor51HI 1394 AGCGCT AGC^GCT
1772 AGCGCT
BanI 1044 GGTGCC G^G[CT][GA]CC
2100 GGCGCC
BbrPI 346 CACGTG CAC^GTG
877 CACGTG
BsaAI 346 CACGTG [CT]AC^GT[GA]
877 CACGTG
BsiEI 1001 CGGCCG CG[GA][CT]^CG
1346 CGACCG
BsmBI 248 GAGACG CGTCTC(1/5)
2185 GAGACG
BsmI 693 GAATGC GAATGC(1/-1)
1868 GCATTC
Bsp1407I 1964 TGTACA T^GTACA
2216 TGTACA
BspMI 914 ACCTGC ACCTGC(4/8)
1172 ACCTGC
BsrGI 1964 TGTACA T^GTACA
2216 TGTACA
BstUI 1071 CGCG CG^CG
1306 CGCG
BstXI 149 CCAAAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1148 CCATGAAGCTGG
BstYI 740 GGATCC [GA]^GATC[CT]
1674 GGATCT
DrdI 415 GACACTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1408 GACCCGGACGTC
Eco47III 1394 AGCGCT AGC^GCT
1772 AGCGCT
EcoRI 721 GAATTC G^AATTC
1627 GAATTC
EcoT14I 1761 CCAAGG C^C[AT][AT]GG
1994 CCAAGG
MflI 740 GGATCC [GA]^GATC[CT]
1674 GGATCT
MslI 1425 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2016 CACCAAAGTG
MvnI 1071 CGCG CG^CG
1306 CGCG
NspI 1176 GCATGT [GA]CATG^[CT]
1931 ACATGT
PflMI 963 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1994 CCAAGGACTGG
PmaCI 346 CACGTG CAC^GTG
877 CACGTG
PmlI 346 CACGTG CAC^GTG
877 CACGTG
PstI 340 CTGCAG CTGCA^G
1427 CTGCAG
PvuII 337 CAGCTG CAG^CTG
2161 CAGCTG
SmaI 81 CCCGGG CCC^GGG
800 CCCGGG
SspBI 1964 TGTACA T^GTACA
2216 TGTACA
StyI 1761 CCAAGG C^C[AT][AT]GG
1994 CCAAGG
Van91I 963 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1994 CCAAGGACTGG
XhoII 740 GGATCC [GA]^GATC[CT]
1674 GGATCT
XmaCI 81 CCCGGG C^CCGGG
800 CCCGGG
XmaI 81 CCCGGG C^CCGGG
800 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI