Restriction map for
pF1KB5678
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
DsaI EagI Eam1105I EclXI Eco52I
EcoO65I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 1361 CGCG CG^CG
AcyI 891 GACGCC G[GA]^CG[CT]C
AflII 1450 CTTAAG C^TTAAG
AocI 914 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1478 AGCGCT AGC^GCT
AseI 754 ATTAAT AT^TAAT
AsnI 754 ATTAAT AT^TAAT
AspHI 880 GTGCTC G[AT]GC[AT]^C
AspI 1769 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1283 GGATCC G^GATCC
BbsI 61 GAAGAC GAAGAC(2/6)
BfrI 1450 CTTAAG C^TTAAG
BpuAI 61 GAAGAC GAAGAC(2/6)
BsaBI 1519 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 891 GACGCC G[GA]^CG[CT]C
BsiHKAI 880 GTGCTC G[AT]GC[AT]^C
BspHI 1712 TCATGA T^CATGA
BsrBI 2226 CCGCTC CCGCTC(-3/-3)
BsrDI 1950 CATTGC GCAATG(2/0)
BsrFI 2251 GCCGGT [GA]^CCGG[CT]
BssSI 1976 CTCGTG CACGAG(-5/-1)
BstAPI 1317 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1361 CGCG CG^CG
Bsu36I 914 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2251 GCCGGT [GA]^CCGG[CT]
DraI 232 TTTAAA TTT^AAA
DrdI 819 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 772 GAAGAG CTCTTC(1/4)
Eco47III 1478 AGCGCT AGC^GCT
Eco81I 914 CCTCAGG CC^T[ACGT]AGG
EcoRI 2186 GAATTC G^AATTC
EcoRV 1498 GATATC GAT^ATC
Hin1I 891 GACGCC G[GA]^CG[CT]C
Ksp632I 772 GAAGAG CTCTTC(1/4)
MamI 1519 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1361 CGCG CG^CG
NspI 1546 GCATGC [GA]CATG^[CT]
PflFI 1769 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 754 ATTAAT AT^TAAT
RcaI 1712 TCATGA T^CATGA
ScaI 1439 AGTACT AGT^ACT
SmaI 2057 CCCGGG CCC^GGG
SmlI 1450 CTTAAG C^T[CT][GA]AG
SphI 1546 GCATGC GCATG^C
Tth111I 1769 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2057 CCCGGG C^CCGGG
XmaI 2057 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 1567 AAATTT [GA]^AATT[CT]
2186 GAATTC
ApoI 1567 AAATTT [GA]^AATT[CT]
2186 GAATTC
BanI 1467 GGTGCC G^G[CT][GA]CC
2308 GGTGCC
BbvCI 46 GCTGAGG CCTCAGC(-5/-2)
2044 GCTGAGG
BglII 104 AGATCT A^GATCT
1803 AGATCT
BseRI 31 CTCCTC GAGGAG(10/8)
1684 CTCCTC
BspMI 1322 ACCTGC ACCTGC(4/8)
1340 ACCTGC
EcoNI 1407 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1743 CCTCTGGGAGG
HaeII 67 AGCGCC [GA]GCGC^[CT]
1478 AGCGCT
HgaI 891 GACGC GACGC(5/10)
1773 GCGTC
MaeII 957 ACGT A^CGT
1542 ACGT
MaeIII 844 GTGAC ^GT[ACGT]AC
1979 GTGAC
PflMI 1076 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1256 CCACTGACTGG
SfiI 432 GGCCTTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
606 GGCCAAAATGGCC
Tsp45I 844 GTGAC ^GT[GC]AC
1979 GTGAC
Van91I 1076 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1256 CCACTGACTGG
XcmI 452 CCACTACTAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2191 CCATTTACCTTCTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
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AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BalI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII