Restriction map for
pF1KB5611
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AvaI AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BlnI BpuAI BsaAI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FseI HaeII
HapII HindIII HpaI HpaII KasI
KpnI KspI MaeI MaeII MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 814 GTCGAC GT^[AC][GT]AC
AciI 505 CCGC CCGC(-3/-1)
AcsI 521 GAATTC [GA]^AATT[CT]
AcyI 976 GGCGTC G[GA]^CG[CT]C
AfaI 930 GTAC GT^AC
AhdI 770 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 764 GATCC GGATC(4/5)
AlwNI 650 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 584 GGGCCC GGGCC^C
ApoI 521 GAATTC [GA]^AATT[CT]
Asp700I 517 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 770 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 985 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 625 GGTCC G^G[AT]CC
AviII 1001 TGCGCA TGC^GCA
BclI 690 TGATCA T^GATCA
BglII 676 AGATCT A^GATCT
BlpI 75 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 75 GCTAAGC GC^T[ACGT]AGC
BsaBI 858 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 976 GGCGTC G[GA]^CG[CT]C
BsgI 362 GTGCAG GTGCAG(16/14)
BsmFI 979 GTCCC GGGAC(10/14)
BspMI 532 GCAGGT ACCTGC(4/8)
BsrDI 803 GCAATG GCAATG(2/0)
CelII 75 GCTAAGC GC^T[ACGT]AGC
CfoI 1002 GCGC GCG^C
DraI 253 TTTAAA TTT^AAA
DsaI 615 CCATGG C^C[GA][CT]GG
Eam1105I 770 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 742 GAAGAG CTCTTC(1/4)
EcoRI 521 GAATTC G^AATTC
FbaI 690 TGATCA T^GATCA
FspI 1001 TGCGCA TGC^GCA
HaeIII 585 GGCC GG^CC
HgaI 977 GCGTC GACGC(5/10)
HhaI 1002 GCGC GCG^C
Hin1I 976 GGCGTC G[GA]^CG[CT]C
HinP1I 1002 GCGC G^CGC
Ksp632I 742 GAAGAG CTCTTC(1/4)
MaeIII 778 GTCAC ^GT[ACGT]AC
MamI 858 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 849 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 615 CCATGG C^CATGG
NlaIV 584 GGGCCC GG[ACGT]^[ACGT]CC
PflFI 985 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 624 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 263 CTGCAG CTGCA^G
PvuII 383 CAGCTG CAG^CTG
RsaI 930 GTAC GT^AC
SacI 745 GAGCTC GAGCT^C
SalI 814 GTCGAC G^TCGAC
SapI 742 GAAGAGC GCTCTTC(1/4)
Tsp45I 778 GTCAC ^GT[GC]AC
Tth111I 985 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 517 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 269 GAGCAC G[AT]GC[AT]^C
745 GAGCTC
BanII 584 GGGCCC G[GA]GC[CT]^C
745 GAGCTC
BbvI 80 GCAGC GCAGC(8/12)
636 GCAGC
BpmI 316 CTGGAG CTGGAG(16/14)
508 CTGGAG
BsiHKAI 269 GAGCAC G[AT]GC[AT]^C
745 GAGCTC
BsmI 528 GAATGC GAATGC(1/-1)
937 GAATGC
BstYI 676 AGATCT [GA]^GATC[CT]
763 AGATCC
EcoT14I 588 CCTTGG C^C[AT][AT]GG
615 CCATGG
Fnu4HI 80 GCAGC GC^[ACGT]GC
636 GCAGC
HincII 378 GTCAAC GT[CT]^[GA]AC
814 GTCGAC
HindII 378 GTCAAC GT[CT]^[GA]AC
814 GTCGAC
ItaI 80 GCAGC GC^[ACGT]GC
636 GCAGC
MflI 676 AGATCT [GA]^GATC[CT]
763 AGATCC
MspA1I 383 CAGCTG C[AC]G^C[GT]G
505 CCGCTG
PleI 436 GACTC GAGTC(4/5)
1120 GACTC
SfaNI 112 GATGC GCATC(5/9)
999 GATGC
SfcI 263 CTGCAG C^T[GA][CT]AG
386 CTGTAG
SmlI 438 CTCAAG C^T[CT][GA]AG
790 CTTGAG
SphI 83 GCATGC GCATG^C
183 GCATGC
StyI 588 CCTTGG C^C[AT][AT]GG
615 CCATGG
TseI 80 GCAGC G^C[AT]GC
636 GCAGC
XhoII 676 AGATCT [GA]^GATC[CT]
763 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FokI HinfI HphI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NspI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I