Restriction map for
pF1KB5542
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbvCI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SalI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwI 946 GGATC GGATC(4/5)
AocI 663 CCTGAGG CC^T[ACGT]AGG
Asp700I 78 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 862 CCCGAG C^[CT]CG[GA]G
BbeI 207 GGCGCC GGCGC^C
BbsI 544 GAAGAC GAAGAC(2/6)
BcgI 191 CGAGGATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 485 AGATCT A^GATCT
BpuAI 544 GAAGAC GAAGAC(2/6)
BsgI 844 GTGCAG GTGCAG(16/14)
BsiEI 108 CGGCCG CG[GA][CT]^CG
BsmBI 592 GAGACG CGTCTC(1/5)
BsoBI 862 CCCGAG C^[CT]CG[GA]G
BsrDI 924 CATTGC GCAATG(2/0)
BsrFI 888 GCCGGC [GA]^CCGG[CT]
BsrI 156 CCAGT ACTGG(1/-1)
BssSI 751 CTCGTG CACGAG(-5/-1)
BstAPI 348 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 663 CCTGAGG CC^T[ACGT]AGG
Cfr10I 888 GCCGGC [GA]^CCGG[CT]
EaeI 108 CGGCCG [CT]^GGCC[GA]
EagI 108 CGGCCG C^GGCCG
EarI 993 GAAGAG CTCTTC(1/4)
EclXI 108 CGGCCG C^GGCCG
Eco52I 108 CGGCCG C^GGCCG
Eco81I 663 CCTGAGG CC^T[ACGT]AGG
EcoT14I 942 CCTTGG C^C[AT][AT]GG
EcoT22I 355 ATGCAT ATGCA^T
KasI 207 GGCGCC G^GCGCC
Ksp632I 993 GAAGAG CTCTTC(1/4)
NaeI 888 GCCGGC GCC^GGC
NarI 207 GGCGCC GG^CGCC
NgoMIV 888 GCCGGC G^CCGGC
NsiI 355 ATGCAT ATGCA^T
NspI 550 ACATGC [GA]CATG^[CT]
PpuMI 446 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 797 CAGCTG CAG^CTG
SapI 993 GAAGAGC GCTCTTC(1/4)
SbfI 766 CCTGCAGG CCTGCA^GG
SfoI 207 GGCGCC GGC^GCC
Sse8387I 766 CCTGCAGG CCTGCA^GG
StuI 763 AGGCCT AGG^CCT
StyI 942 CCTTGG C^C[AT][AT]GG
TaqI 170 TCGA T^CGA
TthHB8I 170 TCGA T^CGA
XcmI 905 CCATCCCCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 78 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 178 GACGCC G[GA]^CG[CT]C
207 GGCGCC
AflIII 624 ACACGT A^C[GA][CT]GT
833 ACACGT
ApaI 373 GGGCCC GGGCC^C
674 GGGCCC
BbrPI 625 CACGTG CAC^GTG
834 CACGTG
BglI 375 GCCCGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
511 GCCTCTGAGGC
BsaHI 178 GACGCC G[GA]^CG[CT]C
207 GGCGCC
BseRI 25 CTCCTC GAGGAG(10/8)
820 GAGGAG
BspMI 293 ACCTGC ACCTGC(4/8)
449 ACCTGC
BstYI 485 AGATCT [GA]^GATC[CT]
946 GGATCT
Hin1I 178 GACGCC G[GA]^CG[CT]C
207 GGCGCC
MaeIII 628 GTGAC ^GT[ACGT]AC
871 GTCAC
MflI 485 AGATCT [GA]^GATC[CT]
946 GGATCT
PmaCI 625 CACGTG CAC^GTG
834 CACGTG
PmlI 625 CACGTG CAC^GTG
834 CACGTG
PstI 346 CTGCAG CTGCA^G
767 CTGCAG
SacI 748 GAGCTC GAGCT^C
996 GAGCTC
SfcI 346 CTGCAG C^T[GA][CT]AG
767 CTGCAG
Tsp45I 628 GTGAC ^GT[GC]AC
871 GTCAC
XhoII 485 AGATCT [GA]^GATC[CT]
946 GGATCT
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KspI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI SacII Sau3AI
Sau96I ScrFI SfaNI TseI TspRI