Restriction map for
pF1KB5527
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BfrI BlnI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 880 GTCTAC GT^[AC][GT]AC
AflIII 1760 ACGTGT A^C[GA][CT]GT
AocI 1360 CCTGAGG CC^T[ACGT]AGG
BcgI 531 CGACAGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2185 GGATAC GTATCC(6/5)
BglI 1129 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 903 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 903 GCTTAGC GC^T[ACGT]AGC
BsaAI 1801 TACGTG [CT]AC^GT[GA]
BsaBI 46 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1826 GAGACC GGTCTC(1/5)
BsiEI 642 CGACCG CG[GA][CT]^CG
BstEII 1260 GGTCACC G^GT[ACGT]ACC
BstPI 1260 GGTCACC G^GT[ACGT]ACC
BstXI 1737 CCAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1360 CCTGAGG CC^T[ACGT]AGG
CelII 903 GCTTAGC GC^T[ACGT]AGC
DrdI 180 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1360 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1260 GGTCACC G^GT[ACGT]ACC
EcoRI 1944 GAATTC G^AATTC
HincII 1930 GTCAAC GT[CT]^[GA]AC
HindII 1930 GTCAAC GT[CT]^[GA]AC
KspI 662 CCGCGG CCGC^GG
MamI 46 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1949 CAATTG C^AATTG
MseI 1844 TTAA T^TAA
MunI 1949 CAATTG C^AATTG
NspI 1215 GCATGC [GA]CATG^[CT]
SacII 662 CCGCGG CCGC^GG
SexAI 914 ACCTGGT A^CC[AT]GGT
SmlI 2176 CTCAAG C^T[CT][GA]AG
SphI 1215 GCATGC GCATG^C
Tru9I 1844 TTAA T^TAA
XcmI 1961 CCAAAGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1731 GAATTT [GA]^AATT[CT]
1944 GAATTC
AcyI 1137 GGCGTC G[GA]^CG[CT]C
1560 GGCGTC
Aor51HI 116 AGCGCT AGC^GCT
767 AGCGCT
ApoI 1731 GAATTT [GA]^AATT[CT]
1944 GAATTC
Asp700I 546 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2136 GAATGACTTC
AspI 678 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1480 GACATGGTC
BalI 2000 TGGCCA TGG^CCA
2148 TGGCCA
BbsI 1384 GAAGAC GAAGAC(2/6)
298 GTCTTC
BglII 548 AGATCT A^GATCT
2252 AGATCT
BpuAI 1384 GAAGAC GAAGAC(2/6)
298 GTCTTC
BsaHI 1137 GGCGTC G[GA]^CG[CT]C
1560 GGCGTC
BspMI 941 ACCTGC ACCTGC(4/8)
1211 ACCTGC
DraIII 358 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1624 CACAACGTG
EaeI 2000 TGGCCA [CT]^GGCC[GA]
2148 TGGCCA
EarI 924 CTCTTC CTCTTC(1/4)
1093 CTCTTC
Eco47III 116 AGCGCT AGC^GCT
767 AGCGCT
Hin1I 1137 GGCGTC G[GA]^CG[CT]C
1560 GGCGTC
Ksp632I 924 CTCTTC CTCTTC(1/4)
1093 CTCTTC
MluNI 2000 TGGCCA TGG^CCA
2148 TGGCCA
MscI 2000 TGGCCA TGG^CCA
2148 TGGCCA
NheI 957 GCTAGC G^CTAGC
1461 GCTAGC
PflFI 678 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1480 GACATGGTC
PshAI 406 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2013 GACTGCTGTC
PstI 493 CTGCAG CTGCA^G
709 CTGCAG
SacI 511 GAGCTC GAGCT^C
1471 GAGCTC
StuI 605 AGGCCT AGG^CCT
1544 AGGCCT
Tth111I 678 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1480 GACATGGTC
XmnI 546 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2136 GAATGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII