Restriction map for
pF1KB5511
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AspI BamHI BbeI BbrPI BcgI
BfrI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 207 GACGTC GACGT^C
AccII 773 CGCG CG^CG
AcsI 1840 AAATTC [GA]^AATT[CT]
AlwNI 622 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1054 CCTGAGG CC^T[ACGT]AGG
Aor51HI 134 AGCGCT AGC^GCT
ApoI 1840 AAATTC [GA]^AATT[CT]
Asp700I 342 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 821 TGCGCA TGC^GCA
AvrII 177 CCTAGG C^CTAGG
BanI 2146 GGCACC G^G[CT][GA]CC
BbsI 373 GTCTTC GAAGAC(2/6)
BciVI 423 GTATCC GTATCC(6/5)
BclI 1367 TGATCA T^GATCA
BglI 827 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 177 CCTAGG C^CTAGG
BlpI 137 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 137 GCTCAGC GC^T[ACGT]AGC
BpuAI 373 GTCTTC GAAGAC(2/6)
BsaAI 1300 TACGTG [CT]AC^GT[GA]
BsaBI 585 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 426 TCCGGT [AT]^CCGG[AT]
BsmBI 1645 GAGACG CGTCTC(1/5)
BsmI 1071 GAATGC GAATGC(1/-1)
BsrBI 1825 GAGCGG CCGCTC(-3/-3)
BssSI 973 CACGAG CACGAG(-5/-1)
BstBI 787 TTCGAA TT^CGAA
BstUI 773 CGCG CG^CG
Bsu36I 1054 CCTGAGG CC^T[ACGT]AGG
CelII 137 GCTCAGC GC^T[ACGT]AGC
DraIII 801 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 134 AGCGCT AGC^GCT
Eco81I 1054 CCTGAGG CC^T[ACGT]AGG
FbaI 1367 TGATCA T^GATCA
FspI 821 TGCGCA TGC^GCA
MaeIII 809 GTGAC ^GT[ACGT]AC
MamI 585 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 773 CGCG CG^CG
NaeI 1106 GCCGGC GCC^GGC
NcoI 720 CCATGG C^CATGG
NgoMIV 1106 GCCGGC G^CCGGC
NspI 950 ACATGC [GA]CATG^[CT]
NspV 787 TTCGAA TT^CGAA
PflMI 621 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfuI 787 TTCGAA TT^CGAA
SmaI 329 CCCGGG CCC^GGG
TfiI 119 GATTC G^A[AT]TC
Tsp45I 809 GTGAC ^GT[GC]AC
Tsp509I 1841 AATT ^AATT
Van91I 621 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 329 CCCGGG C^CCGGG
XmaI 329 CCCGGG C^CCGGG
XmnI 342 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 1599 GTAC GT^AC
1854 GTAC
AvaI 329 CCCGGG C^[CT]CG[GA]G
1579 CCCGAG
BbvCI 489 CCTCAGC CCTCAGC(-5/-2)
382 GCTGAGG
BfaI 178 CTAG C^TAG
1522 CTAG
BglII 554 AGATCT A^GATCT
1407 AGATCT
BseRI 385 GAGGAG GAGGAG(10/8)
1711 GAGGAG
BsmFI 205 GGGAC GGGAC(10/14)
903 GTCCC
BsoBI 329 CCCGGG C^[CT]CG[GA]G
1579 CCCGAG
BspMI 1544 ACCTGC ACCTGC(4/8)
214 GCAGGT
BsrDI 1209 CATTGC GCAATG(2/0)
1857 CATTGC
BsrFI 1106 GCCGGC [GA]^CCGG[CT]
1493 ACCGGC
Cfr10I 1106 GCCGGC [GA]^CCGG[CT]
1493 ACCGGC
DsaI 720 CCATGG C^C[GA][CT]GG
857 CCACGG
EcoNI 177 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1557 CCTCTGGGAGG
HaeII 134 AGCGCT [GA]GCGC^[CT]
168 GGCGCT
HgaI 61 GACGC GACGC(5/10)
1647 GACGC
MaeI 178 CTAG C^TAG
1522 CTAG
PleI 2108 GACTC GAGTC(4/5)
2009 GAGTC
PstI 742 CTGCAG CTGCA^G
1396 CTGCAG
PvuII 538 CAGCTG CAG^CTG
1119 CAGCTG
RsaI 1599 GTAC GT^AC
1854 GTAC
SacI 334 GAGCTC GAGCT^C
388 GAGCTC
SfcI 742 CTGCAG C^T[GA][CT]AG
1396 CTGCAG
SmlI 655 CTCAAG C^T[CT][GA]AG
829 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcyI AluI AlwI ApaI
AspHI AvaII BalI BanII BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
TspRI TthHB8I XcmI XhoII