Restriction map for
pF1KB5444
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbeI
BbrPI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HapII Hin1I HpaI
HpaII KasI KspI MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 532 GGTACC G^GTACC
AccI 259 GTAGAC GT^[AC][GT]AC
Alw44I 13 GTGCAC G^TGCAC
AlwNI 2108 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 13 GTGCAC G^TGCAC
Asp718I 532 GGTACC G^GTACC
BamHI 2013 GGATCC G^GATCC
BanI 532 GGTACC G^G[CT][GA]CC
BbvCI 1924 GCTGAGG CCTCAGC(-5/-2)
BcgI 2139 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 46 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1599 GGTCTC GGTCTC(1/5)
BspMI 1476 GCAGGT ACCTGC(4/8)
BsrBI 1807 GAGCGG CCGCTC(-3/-3)
BstAPI 55 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 186 TTCGAA TT^CGAA
CfoI 1365 GCGC GCG^C
DraII 1955 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1955 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 588 GCGGG CCCGC(4/6)
HhaI 1365 GCGC GCG^C
HinP1I 1365 GCGC G^CGC
HincII 1141 GTTGAC GT[CT]^[GA]AC
HindII 1141 GTTGAC GT[CT]^[GA]AC
HindIII 1312 AAGCTT A^AGCTT
KpnI 532 GGTACC GGTAC^C
MaeII 243 ACGT A^CGT
NspV 186 TTCGAA TT^CGAA
PaeR7I 2131 CTCGAG C^TCGAG
PpuMI 1955 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1748 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2105 CTGCAG CTGCA^G
PvuII 1092 CAGCTG CAG^CTG
SacI 1240 GAGCTC GAGCT^C
ScaI 1556 AGTACT AGT^ACT
SexAI 1958 ACCTGGT A^CC[AT]GGT
SfuI 186 TTCGAA TT^CGAA
XhoI 2131 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 13 GTGCAC G[AT]GC[AT]^C
1240 GAGCTC
AvaI 1172 CCCGAG C^[CT]CG[GA]G
2131 CTCGAG
AvaII 866 GGACC G^G[AT]CC
1956 GGACC
BalI 1481 TGGCCA TGG^CCA
1862 TGGCCA
BsgI 359 GTGCAG GTGCAG(16/14)
1564 GTGCAG
BsiHKAI 13 GTGCAC G[AT]GC[AT]^C
1240 GAGCTC
BsmBI 2150 CGTCTC CGTCTC(1/5)
1906 GAGACG
BsmFI 1835 GGGAC GGGAC(10/14)
75 GTCCC
BsoBI 1172 CCCGAG C^[CT]CG[GA]G
2131 CTCGAG
BspHI 1697 TCATGA T^CATGA
1814 TCATGA
BsrDI 312 CATTGC GCAATG(2/0)
992 CATTGC
Cfr13I 866 GGACC G^G[ACGT]CC
1956 GGACC
DrdI 1398 GACATCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1434 GACACAGAAGTC
EaeI 1481 TGGCCA [CT]^GGCC[GA]
1862 TGGCCA
EcoRI 907 GAATTC G^AATTC
982 GAATTC
HgaI 1908 GACGC GACGC(5/10)
2149 GCGTC
MluNI 1481 TGGCCA TGG^CCA
1862 TGGCCA
MscI 1481 TGGCCA TGG^CCA
1862 TGGCCA
MspA1I 780 CAGCGG C[AC]G^C[GT]G
1092 CAGCTG
RcaI 1697 TCATGA T^CATGA
1814 TCATGA
Sau96I 866 GGACC G^G[ACGT]CC
1956 GGACC
SfcI 965 CTGTAG C^T[GA][CT]AG
2105 CTGCAG
TfiI 159 GATTC G^A[AT]TC
1319 GAATC
Tsp45I 1732 GTGAC ^GT[GC]AC
1774 GTGAC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI BanII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I DdeI
DpnII DsaI EarI Eco81I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI RsaI
SapI Sau3AI ScrFI SfaNI SmlI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII