Restriction map for
pF1KB5397
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 19 CGCG CG^CG
AfaI 885 GTAC GT^AC
AspHI 265 GAGCAC G[AT]GC[AT]^C
AspI 487 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 57 CTCGGG C^[CT]CG[GA]G
AvaII 648 GGACC G^G[AT]CC
BamHI 892 GGATCC G^GATCC
BanII 311 GAGCCC G[GA]GC[CT]^C
BbvCI 12 GCTGAGG CCTCAGC(-5/-2)
BciVI 454 GGATAC GTATCC(6/5)
BglI 313 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 265 GAGCAC G[AT]GC[AT]^C
BsmI 515 GCATTC GAATGC(1/-1)
BsoBI 57 CTCGGG C^[CT]CG[GA]G
BspHI 908 TCATGA T^CATGA
BsrFI 687 GCCGGT [GA]^CCGG[CT]
BstUI 19 CGCG CG^CG
Cfr10I 687 GCCGGT [GA]^CCGG[CT]
HaeII 824 AGCGCC [GA]GCGC^[CT]
MboII 146 GAAGA GAAGA(8/7)
MslI 270 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 316 CAGCTG C[AC]G^C[GT]G
MvnI 19 CGCG CG^CG
NspI 749 GCATGT [GA]CATG^[CT]
PflFI 487 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 547 GAGTC GAGTC(4/5)
PpuMI 647 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 316 CAGCTG CAG^CTG
RcaI 908 TCATGA T^CATGA
RsaI 885 GTAC GT^AC
TaqI 818 TCGA T^CGA
Tth111I 487 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 818 TCGA T^CGA
XcmI 218 CCATCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BcnI 422 CCCGG CC^[GC]GG
506 CCCGG
BpmI 568 CTCCAG CTGGAG(16/14)
619 CTGGAG
BseRI 4 GAGGAG GAGGAG(10/8)
389 GAGGAG
BsmFI 554 GGGAC GGGAC(10/14)
647 GGGAC
BstYI 596 AGATCC [GA]^GATC[CT]
892 GGATCC
DdeI 13 CTGAG C^T[ACGT]AG
286 CTGAG
EaeI 320 TGGCCG [CT]^GGCC[GA]
717 CGGCCA
EcoNI 363 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
459 CCTCCCCCAGG
EcoT14I 131 CCAAGG C^C[AT][AT]GG
803 CCAAGG
HindIII 304 AAGCTT A^AGCTT
346 AAGCTT
MaeIII 442 GTGAC ^GT[ACGT]AC
614 GTGAC
MflI 596 AGATCC [GA]^GATC[CT]
892 GGATCC
MseI 175 TTAA T^TAA
592 TTAA
NciI 422 CCCGG CC^[GC]GG
506 CCCGG
PstI 30 CTGCAG CTGCA^G
586 CTGCAG
SfcI 30 CTGCAG C^T[GA][CT]AG
586 CTGCAG
StyI 131 CCAAGG C^C[AT][AT]GG
803 CCAAGG
Tru9I 175 TTAA T^TAA
592 TTAA
Tsp45I 442 GTGAC ^GT[GC]AC
614 GTGAC
TspRI 380 CAGTG CA[GC]TG[ACGT][ACGT]^
862 CACTG
XhoII 596 AGATCC [GA]^GATC[CT]
892 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII DraII DsaI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TfiI TseI