Restriction map for
pF1KB5353
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AviII
BamHI BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssKI
BssSI Bst1107I BstAPI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI KspI
MfeI MluI MroI MslI MspA1I
MspI MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AciI 1081 GCGG CCGC(-3/-1)
Alw44I 263 GTGCAC G^TGCAC
AlwNI 611 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 263 GTGCAC G^TGCAC
AseI 361 ATTAAT AT^TAAT
AsnI 361 ATTAAT AT^TAAT
Asp700I 568 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 263 GTGCAC G[AT]GC[AT]^C
AvaII 745 GGACC G^G[AT]CC
AvrII 211 CCTAGG C^CTAGG
BalI 618 TGGCCA TGG^CCA
BanI 770 GGTGCC G^G[CT][GA]CC
BbsI 484 GAAGAC GAAGAC(2/6)
BciVI 193 GGATAC GTATCC(6/5)
BclI 36 TGATCA T^GATCA
BfaI 212 CTAG C^TAG
BglII 930 AGATCT A^GATCT
BlnI 211 CCTAGG C^CTAGG
BmyI 263 GTGCAC G[AGT]GC[ACT]^C
BpmI 1028 CTGGAG CTGGAG(16/14)
BpuAI 484 GAAGAC GAAGAC(2/6)
BsaAI 989 TACGTG [CT]AC^GT[GA]
BsaBI 721 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 211 CCTAGG C^C[ACGT][ACGT]GG
BsiHKAI 263 GTGCAC G[AT]GC[AT]^C
BsmI 234 GAATGC GAATGC(1/-1)
Bsp1286I 263 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 763 TGTACA T^GTACA
BspHI 297 TCATGA T^CATGA
BspMI 248 ACCTGC ACCTGC(4/8)
BsrGI 763 TGTACA T^GTACA
BstBI 970 TTCGAA TT^CGAA
BstYI 930 AGATCT [GA]^GATC[CT]
Cfr13I 745 GGACC G^G[ACGT]CC
DrdI 164 GACTTGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 618 TGGCCA [CT]^GGCC[GA]
EarI 70 GAAGAG CTCTTC(1/4)
EcoRI 497 GAATTC G^AATTC
EcoT14I 211 CCTAGG C^C[AT][AT]GG
FbaI 36 TGATCA T^GATCA
HpaI 513 GTTAAC GTT^AAC
Ksp632I 70 GAAGAG CTCTTC(1/4)
MaeI 212 CTAG C^TAG
MamI 721 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 930 AGATCT [GA]^GATC[CT]
MluNI 618 TGGCCA TGG^CCA
MscI 618 TGGCCA TGG^CCA
MwoI 952 GCTATCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 298 CATG CATG^
NlaIV 770 GGTGCC GG[ACGT]^[ACGT]CC
NspV 970 TTCGAA TT^CGAA
PleI 883 GACTC GAGTC(4/5)
PshBI 361 ATTAAT AT^TAAT
PstI 962 CTGCAG CTGCA^G
RcaI 297 TCATGA T^CATGA
Sau96I 745 GGACC G^G[ACGT]CC
SfcI 962 CTGCAG C^T[GA][CT]AG
SfuI 970 TTCGAA TT^CGAA
SspBI 763 TGTACA T^GTACA
SspI 665 AATATT AAT^ATT
StyI 211 CCTAGG C^C[AT][AT]GG
TaqI 971 TCGA T^CGA
TthHB8I 971 TCGA T^CGA
XhoII 930 AGATCT [GA]^GATC[CT]
XmnI 568 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 764 GTAC GT^AC
857 GTAC
BsmFI 840 GGGAC GGGAC(10/14)
847 GGGAC
BsrI 79 ACTGG ACTGG(1/-1)
1027 ACTGG
HaeIII 447 GGCC GG^CC
619 GGCC
HincII 513 GTTAAC GT[CT]^[GA]AC
532 GTTGAC
HindII 513 GTTAAC GT[CT]^[GA]AC
532 GTTGAC
HphI 82 GGTGA GGTGA(8/7)
1072 GGTGA
RsaI 764 GTAC GT^AC
857 GTAC
TfiI 141 GATTC G^A[AT]TC
721 GATTC
TspRI 543 CAGTG CA[GC]TG[ACGT][ACGT]^
1013 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BsiYI BslI BstF5I Cac8I DdeI
DpnII Fnu4HI FokI HinfI ItaI
MaeII MaeIII MboI MboII MnlI
MseI NdeII Sau3AI SfaNI Tru9I
TseI Tsp45I Tsp509I