Restriction map for
pF1KB5331
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# NO CUT ENZYMES ( 135 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1066 GACGTC GACGT^C
AccI 1084 GTAGAC GT^[AC][GT]AC
AcyI 1066 GACGTC G[GA]^CG[CT]C
AgeI 211 ACCGGT A^CCGGT
AlwNI 88 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 861 GAGCAC G[AT]GC[AT]^C
AviII 932 TGCGCA TGC^GCA
AvrII 837 CCTAGG C^CTAGG
BciVI 1191 GGATAC GTATCC(6/5)
BlnI 837 CCTAGG C^CTAGG
BlpI 478 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 478 GCTGAGC GC^T[ACGT]AGC
BsaAI 708 CACGTA [CT]AC^GT[GA]
BsaBI 96 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1066 GACGTC G[GA]^CG[CT]C
BsaWI 211 ACCGGT [AT]^CCGG[AT]
BsiHKAI 861 GAGCAC G[AT]GC[AT]^C
BsmI 662 GCATTC GAATGC(1/-1)
BspMI 224 GCAGGT ACCTGC(4/8)
BsrBI 469 GAGCGG CCGCTC(-3/-3)
BsrI 123 CCAGT ACTGG(1/-1)
BstYI 699 GGATCT [GA]^GATC[CT]
CelII 478 GCTGAGC GC^T[ACGT]AGC
CpoI 472 CGGACCG CG^G[AT]CCG
EarI 28 GAAGAG CTCTTC(1/4)
FspI 932 TGCGCA TGC^GCA
HaeII 1004 AGCGCC [GA]GCGC^[CT]
Hin1I 1066 GACGTC G[GA]^CG[CT]C
Ksp632I 28 GAAGAG CTCTTC(1/4)
MamI 96 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 699 GGATCT [GA]^GATC[CT]
NspI 332 GCATGT [GA]CATG^[CT]
PinAI 211 ACCGGT A^CCGGT
RsrII 472 CGGACCG CG^G[AT]CCG
SgrAI 210 CACCGGTG C[GA]^CCGG[CT]G
XhoII 699 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 911 CGCG CG^CG
913 CGCG
AflIII 566 ACGTGT A^C[GA][CT]GT
707 ACACGT
ApaI 119 GGGCCC GGGCC^C
1210 GGGCCC
BbsI 1162 GAAGAC GAAGAC(2/6)
1069 GTCTTC
BfaI 457 CTAG C^TAG
838 CTAG
BpmI 466 CTGGAG CTGGAG(16/14)
946 CTGGAG
BpuAI 1162 GAAGAC GAAGAC(2/6)
1069 GTCTTC
BsrDI 831 CATTGC GCAATG(2/0)
434 GCAATG
BssHII 910 GCGCGC G^CGCGC
912 GCGCGC
BssSI 1196 CACGAG CACGAG(-5/-1)
1009 CTCGTG
BstEII 66 GGTGACC G^GT[ACGT]ACC
288 GGTGACC
BstPI 66 GGTGACC G^GT[ACGT]ACC
288 GGTGACC
BstUI 911 CGCG CG^CG
913 CGCG
DraII 118 GGGGCCC [GA]G^G[ACGT]CC[CT]
1210 GGGCCCT
EcoNI 72 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1116 CCTCAACCAGG
EcoO109I 118 GGGGCCC [GA]G^G[ACGT]CC[CT]
1210 GGGCCCT
EcoO65I 66 GGTGACC G^GT[ACGT]ACC
288 GGTGACC
MaeI 457 CTAG C^TAG
838 CTAG
MvnI 911 CGCG CG^CG
913 CGCG
MwoI 456 GCTAGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
607 GCGAGCGAGGC
PleI 546 GACTC GAGTC(4/5)
1240 GACTC
ScaI 125 AGTACT AGT^ACT
1028 AGTACT
TfiI 44 GAATC G^A[AT]TC
267 GATTC
TspRI 188 CAGTG CA[GC]TG[ACGT][ACGT]^
1049 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrFI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TthHB8I