Restriction map for
pF1KB5266
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraII EagI Eam1105I EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HindIII KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 41 CGCG CG^CG
AcyI 15 GACGCC G[GA]^CG[CT]C
AlwI 507 GGATC GGATC(4/5)
Aor51HI 100 AGCGCT AGC^GCT
Asp700I 568 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 33 TGGCCA TGG^CCA
BcnI 350 CCGGG CC^[GC]GG
BfaI 988 CTAG C^TAG
BsaHI 15 GACGCC G[GA]^CG[CT]C
BseRI 982 GAGGAG GAGGAG(10/8)
BspMI 25 ACCTGC ACCTGC(4/8)
BsrBI 19 CCGCTC CCGCTC(-3/-3)
BsrDI 4 GCAATG GCAATG(2/0)
BstUI 41 CGCG CG^CG
CfoI 101 GCGC GCG^C
DraI 733 TTTAAA TTT^AAA
DraIII 805 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 788 GACACTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 47 CCGTGG C^C[GA][CT]GG
EaeI 33 TGGCCA [CT]^GGCC[GA]
Eco47III 100 AGCGCT AGC^GCT
EcoRI 1008 GAATTC G^AATTC
HaeII 100 AGCGCT [GA]GCGC^[CT]
HapII 350 CCGG C^CGG
HgaI 15 GACGC GACGC(5/10)
HhaI 101 GCGC GCG^C
Hin1I 15 GACGCC G[GA]^CG[CT]C
HinP1I 101 GCGC G^CGC
HincII 345 GTTAAC GT[CT]^[GA]AC
HindII 345 GTTAAC GT[CT]^[GA]AC
HpaI 345 GTTAAC GTT^AAC
HpaII 350 CCGG C^CGG
MaeI 988 CTAG C^TAG
MaeII 895 ACGT A^CGT
MaeIII 369 GTTAC ^GT[ACGT]AC
MluNI 33 TGGCCA TGG^CCA
MscI 33 TGGCCA TGG^CCA
MslI 172 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 230 CAGCTG C[AC]G^C[GT]G
MspI 350 CCGG C^CGG
MvnI 41 CGCG CG^CG
NciI 350 CCGGG CC^[GC]GG
NlaIII 511 CATG CATG^
NlaIV 642 GGACCC GG[ACGT]^[ACGT]CC
PstI 233 CTGCAG CTGCA^G
PvuII 230 CAGCTG CAG^CTG
TaqI 498 TCGA T^CGA
TthHB8I 498 TCGA T^CGA
XmnI 568 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AciI 19 CCGC CCGC(-3/-1)
722 GCGG
AlwNI 227 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1031 CAGCTCCTG
AocI 406 CCTAAGG CC^T[ACGT]AGG
577 CCTGAGG
AvaII 642 GGACC G^G[AT]CC
796 GGTCC
BsgI 453 GTGCAG GTGCAG(16/14)
629 GTGCAG
BsiYI 248 CCAAAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
952 CCAAAGATTGG
BslI 248 CCAAAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
952 CCAAAGATTGG
BsmFI 354 GGGAC GGGAC(10/14)
786 GGGAC
BsrI 149 ACTGG ACTGG(1/-1)
842 CCAGT
BstNI 132 CCAGG CC^[AT]GG
288 CCTGG
Bsu36I 406 CCTAAGG CC^T[ACGT]AGG
577 CCTGAGG
Cfr13I 642 GGACC G^G[ACGT]CC
796 GGTCC
DpnII 508 GATC ^GATC
611 GATC
EarI 689 CTCTTC CTCTTC(1/4)
979 GAAGAG
Eco81I 406 CCTAAGG CC^T[ACGT]AGG
577 CCTGAGG
EcoRII 132 CCAGG ^CC[AT]GG
288 CCTGG
HinfI 471 GATTC G^A[ACGT]TC
775 GAATC
Ksp632I 689 CTCTTC CTCTTC(1/4)
979 GAAGAG
MboI 508 GATC ^GATC
611 GATC
MvaI 132 CCAGG CC^[AT]GG
288 CCTGG
NdeII 508 GATC ^GATC
611 GATC
PflMI 248 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCAAAGATTGG
Sau3AI 508 GATC ^GATC
611 GATC
Sau96I 642 GGACC G^G[ACGT]CC
796 GGTCC
SfcI 224 CTACAG C^T[GA][CT]AG
233 CTGCAG
TfiI 471 GATTC G^A[AT]TC
775 GAATC
Van91I 248 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCAAAGATTGG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BsmAI BssKI BstF5I Cac8I DdeI
Fnu4HI FokI HaeIII HphI ItaI
MboII MnlI MseI MwoI ScrFI
SfaNI Tru9I TseI Tsp509I TspRI