Restriction map for
pF1KB5107
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp718I AvrII BbvCI BcgI
BciVI BclI BfrI BlnI BsiEI
BsiWI BsmBI BspDI BspLU11I BsrDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MluI MseI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tru9I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 316 CGCG CG^CG
AccIII 320 TCCGGA T^CCGGA
Alw44I 1594 GTGCAC G^TGCAC
ApaLI 1594 GTGCAC G^TGCAC
Asp700I 1512 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 313 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1648 CCCGAG C^[CT]CG[GA]G
AviII 1658 TGCGCA TGC^GCA
BbeI 1743 GGCGCC GGCGC^C
BglII 128 AGATCT A^GATCT
BpmI 1791 CTGGAG CTGGAG(16/14)
BseAI 320 TCCGGA T^CCGGA
BsmI 1802 GCATTC GAATGC(1/-1)
BsoBI 1648 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1811 TGTACA T^GTACA
BspEI 320 TCCGGA T^CCGGA
BspHI 1712 TCATGA T^CATGA
BsrGI 1811 TGTACA T^GTACA
BstAPI 2001 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2022 GGTCACC G^GT[ACGT]ACC
BstPI 2022 GGTCACC G^GT[ACGT]ACC
BstUI 316 CGCG CG^CG
DraIII 1201 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2022 GGTCACC G^GT[ACGT]ACC
FspI 1658 TGCGCA TGC^GCA
HincII 700 GTCAAC GT[CT]^[GA]AC
HindII 700 GTCAAC GT[CT]^[GA]AC
HindIII 2038 AAGCTT A^AGCTT
KasI 1743 GGCGCC G^GCGCC
MfeI 1268 CAATTG C^AATTG
MroI 320 TCCGGA T^CCGGA
MunI 1268 CAATTG C^AATTG
MvnI 316 CGCG CG^CG
NarI 1743 GGCGCC GG^CGCC
PflFI 313 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 968 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2008 CTGCAG CTGCA^G
RcaI 1712 TCATGA T^CATGA
SacI 187 GAGCTC GAGCT^C
SfoI 1743 GGCGCC GGC^GCC
SphI 839 GCATGC GCATG^C
SspBI 1811 TGTACA T^GTACA
StuI 653 AGGCCT AGG^CCT
Tth111I 313 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 968 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1512 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 115 GGCGTC G[GA]^CG[CT]C
1743 GGCGCC
AhdI 730 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1948 GACCTGGTGTC
AlwNI 847 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2002 CAGGTTCTG
Aor51HI 1105 AGCGCT AGC^GCT
1298 AGCGCT
ApaI 881 GGGCCC GGGCC^C
1112 GGGCCC
AspEI 730 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1948 GACCTGGTGTC
BalI 1145 TGGCCA TGG^CCA
1398 TGGCCA
BbrPI 1450 CACGTG CAC^GTG
1969 CACGTG
BfaI 43 CTAG C^TAG
835 CTAG
BglI 505 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1183 GCCCCGCAGGC
BlpI 1027 GCTCAGC GC^T[ACGT]AGC
1996 GCTAAGC
Bpu1102I 1027 GCTCAGC GC^T[ACGT]AGC
1996 GCTAAGC
BsaBI 129 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
352 GATGTAAATC
BsaHI 115 GGCGTC G[GA]^CG[CT]C
1743 GGCGCC
BsaI 557 GAGACC GGTCTC(1/5)
805 GAGACC
BsaWI 320 TCCGGA [AT]^CCGG[AT]
797 ACCGGA
BspMI 530 ACCTGC ACCTGC(4/8)
2001 GCAGGT
BsrFI 908 ACCGGC [GA]^CCGG[CT]
1150 ACCGGC
BssSI 643 CACGAG CACGAG(-5/-1)
1178 CTCGTG
CelII 1027 GCTCAGC GC^T[ACGT]AGC
1996 GCTAAGC
Cfr10I 908 ACCGGC [GA]^CCGG[CT]
1150 ACCGGC
Eam1105I 730 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1948 GACCTGGTGTC
EarI 445 CTCTTC CTCTTC(1/4)
1372 GAAGAG
Eco47III 1105 AGCGCT AGC^GCT
1298 AGCGCT
HgaI 116 GCGTC GACGC(5/10)
317 GCGTC
Hin1I 115 GGCGTC G[GA]^CG[CT]C
1743 GGCGCC
Ksp632I 445 CTCTTC CTCTTC(1/4)
1372 GAAGAG
MaeI 43 CTAG C^TAG
835 CTAG
MamI 129 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
352 GATGTAAATC
MluNI 1145 TGGCCA TGG^CCA
1398 TGGCCA
MscI 1145 TGGCCA TGG^CCA
1398 TGGCCA
MslI 265 CACCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1317 CACCAACATG
NspI 839 GCATGC [GA]CATG^[CT]
951 GCATGT
PleI 1636 GAGTC GAGTC(4/5)
2152 GAGTC
PmaCI 1450 CACGTG CAC^GTG
1969 CACGTG
PmlI 1450 CACGTG CAC^GTG
1969 CACGTG
SfcI 2008 CTGCAG C^T[GA][CT]AG
2071 CTACAG
TaqI 185 TCGA T^CGA
2150 TCGA
Tsp509I 1269 AATT ^AATT
1918 AATT
TthHB8I 185 TCGA T^CGA
2150 TCGA
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AocI AspHI AvaII BamHI BanI
BanII BbsI BbvI BcnI BmyI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SmlI StyI TfiI TseI
Tsp45I TspRI XcmI XhoII