Restriction map for
pF1KB5103
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BfrI
BglII BlnI BpuAI BseAI BsiEI
BsiWI BspDI BspEI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FauI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SphI Sse8387I SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1486 GTATAC GT^[AC][GT]AC
AcyI 263 GGCGCC G[GA]^CG[CT]C
Alw44I 335 GTGCAC G^TGCAC
AocI 1771 CCTGAGG CC^T[ACGT]AGG
ApaLI 335 GTGCAC G^TGCAC
AvaI 252 CCCGGG C^[CT]CG[GA]G
BanII 1282 GGGCTC G[GA]GC[CT]^C
BbeI 263 GGCGCC GGCGC^C
BcgI 1266 CGAAGTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 976 GTATCC GTATCC(6/5)
BclI 2046 TGATCA T^GATCA
BglI 1079 GCCCTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 852 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 852 GCTAAGC GC^T[ACGT]AGC
BsaAI 1525 TACGTG [CT]AC^GT[GA]
BsaBI 1312 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 263 GGCGCC G[GA]^CG[CT]C
BsaI 398 GAGACC GGTCTC(1/5)
BsaWI 1642 TCCGGT [AT]^CCGG[AT]
BsmBI 721 CGTCTC CGTCTC(1/5)
BsoBI 252 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1094 TGTACA T^GTACA
BspHI 942 TCATGA T^CATGA
BspLU11I 1199 ACATGT A^CATGT
BsrBI 93 GAGCGG CCGCTC(-3/-3)
BsrGI 1094 TGTACA T^GTACA
Bst1107I 1486 GTATAC GTA^TAC
BstZ17I 1486 GTATAC GTA^TAC
Bsu36I 1771 CCTGAGG CC^T[ACGT]AGG
CelII 852 GCTAAGC GC^T[ACGT]AGC
DraII 1621 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 35 TGGCCG [CT]^GGCC[GA]
Eco81I 1771 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1621 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1979 GATATC GAT^ATC
EcoT14I 1225 CCAAGG C^C[AT][AT]GG
FbaI 2046 TGATCA T^GATCA
Hin1I 263 GGCGCC G[GA]^CG[CT]C
KasI 263 GGCGCC G^GCGCC
MamI 1312 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 172 CAGCTG C[AC]G^C[GT]G
NarI 263 GGCGCC GG^CGCC
PpuMI 1621 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 172 CAGCTG CAG^CTG
RcaI 942 TCATGA T^CATGA
ScaI 405 AGTACT AGT^ACT
SfoI 263 GGCGCC GGC^GCC
SmaI 252 CCCGGG CCC^GGG
SpeI 1615 ACTAGT A^CTAGT
SspBI 1094 TGTACA T^GTACA
StyI 1225 CCAAGG C^C[AT][AT]GG
TaqI 1836 TCGA T^CGA
TthHB8I 1836 TCGA T^CGA
XcmI 1716 CCAGACTCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 252 CCCGGG C^CCGGG
XmaI 252 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 1199 ACATGT A^C[GA][CT]GT
1526 ACGTGT
AlwNI 1804 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1827 CAGGCTCTG
AspHI 335 GTGCAC G[AT]GC[AT]^C
1821 GTGCTC
BseRI 105 GAGGAG GAGGAG(10/8)
833 GAGGAG
BsgI 1799 CTGCAC GTGCAG(16/14)
1848 CTGCAC
BsiHKAI 335 GTGCAC G[AT]GC[AT]^C
1821 GTGCTC
BsmFI 1892 GGGAC GGGAC(10/14)
168 GTCCC
BsmI 1777 GAATGC GAATGC(1/-1)
862 GCATTC
BspMI 1797 ACCTGC ACCTGC(4/8)
2035 GCAGGT
BstYI 1749 AGATCC [GA]^GATC[CT]
2079 GGATCT
CfoI 138 GCGC GCG^C
264 GCGC
EarI 724 CTCTTC CTCTTC(1/4)
616 GAAGAG
HaeII 137 AGCGCC [GA]GCGC^[CT]
263 GGCGCC
HhaI 138 GCGC GCG^C
264 GCGC
HinP1I 138 GCGC G^CGC
264 GCGC
Ksp632I 724 CTCTTC CTCTTC(1/4)
616 GAAGAG
MflI 1749 AGATCC [GA]^GATC[CT]
2079 GGATCT
NspI 1199 ACATGT [GA]CATG^[CT]
1924 GCATGT
PstI 1872 CTGCAG CTGCA^G
1899 CTGCAG
XhoII 1749 AGATCC [GA]^GATC[CT]
2079 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BcnI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI Tru9I
TseI Tsp45I Tsp509I TspRI