Restriction map for
pF1KB5083
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AclI AcyI AflII AflIII
AgeI Alw44I ApaLI AscI AspI
AvaII AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstXI Cfr10I
ClaI CpoI DraI DraIII EarI
EcoNI EcoT22I FseI FspI HgaI
Hin1I HpaI KasI Ksp632I KspI
MaeII MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 209 CGCG CG^CG
AccIII 1017 TCCGGA T^CCGGA
AhdI 1021 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 960 GGATC GGATC(4/5)
AocI 555 CCTCAGG CC^T[ACGT]AGG
Aor51HI 239 AGCGCT AGC^GCT
ApaI 791 GGGCCC GGGCC^C
AseI 637 ATTAAT AT^TAAT
AsnI 637 ATTAAT AT^TAAT
Asp700I 1918 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1021 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1650 TGGCCA TGG^CCA
BclI 1881 TGATCA T^GATCA
BlpI 1488 GCTGAGC GC^T[ACGT]AGC
BpmI 1258 CTCCAG CTGGAG(16/14)
Bpu1102I 1488 GCTGAGC GC^T[ACGT]AGC
BsaWI 1017 TCCGGA [AT]^CCGG[AT]
BseAI 1017 TCCGGA T^CCGGA
BsgI 427 GTGCAG GTGCAG(16/14)
BsiEI 100 CGGCCG CG[GA][CT]^CG
BspEI 1017 TCCGGA T^CCGGA
BspHI 588 TCATGA T^CATGA
BsrDI 905 CATTGC GCAATG(2/0)
BstBI 1784 TTCGAA TT^CGAA
BstEII 1509 GGTCACC G^GT[ACGT]ACC
BstPI 1509 GGTCACC G^GT[ACGT]ACC
BstUI 209 CGCG CG^CG
Bsu36I 555 CCTCAGG CC^T[ACGT]AGG
CelII 1488 GCTGAGC GC^T[ACGT]AGC
DrdI 1564 GACCACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 100 CGGCCG C^GGCCG
Eam1105I 1021 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 100 CGGCCG C^GGCCG
Eco47III 239 AGCGCT AGC^GCT
Eco52I 100 CGGCCG C^GGCCG
Eco81I 555 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1509 GGTCACC G^GT[ACGT]ACC
EcoRI 404 GAATTC G^AATTC
EcoRV 1950 GATATC GAT^ATC
FauI 1297 CCCGC CCCGC(4/6)
FbaI 1881 TGATCA T^GATCA
HindIII 1189 AAGCTT A^AGCTT
MluNI 1650 TGGCCA TGG^CCA
MroI 1017 TCCGGA T^CCGGA
MscI 1650 TGGCCA TGG^CCA
MvnI 209 CGCG CG^CG
NotI 99 GCGGCCGC GC^GGCCGC
NspV 1784 TTCGAA TT^CGAA
PshAI 667 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 637 ATTAAT AT^TAAT
PstI 483 CTGCAG CTGCA^G
RcaI 588 TCATGA T^CATGA
SacI 1255 GAGCTC GAGCT^C
SalI 220 GTCGAC G^TCGAC
SfuI 1784 TTCGAA TT^CGAA
XmnI 1918 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 724 GGTACC G^GTACC
1550 GGTACC
AlwNI 58 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1100 CAGCTGCTG
Asp718I 724 GGTACC G^GTACC
1550 GGTACC
AvaI 115 CCCGAG C^[CT]CG[GA]G
192 CTCGGG
BanII 791 GGGCCC G[GA]GC[CT]^C
1255 GAGCTC
BcnI 156 CCCGG CC^[GC]GG
249 CCGGG
BseRI 1252 GAGGAG GAGGAG(10/8)
1738 GAGGAG
BsiYI 226 CCCACGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
227 CCACGACTGGG
BslI 226 CCCACGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
227 CCACGACTGGG
BsmI 1381 GAATGC GAATGC(1/-1)
1472 GCATTC
BsoBI 115 CCCGAG C^[CT]CG[GA]G
192 CTCGGG
BsrI 232 ACTGG ACTGG(1/-1)
968 CCAGT
Bst1107I 810 GTATAC GTA^TAC
888 GTATAC
BstZ17I 810 GTATAC GTA^TAC
888 GTATAC
DsaI 647 CCATGG C^C[GA][CT]GG
1400 CCATGG
EaeI 100 CGGCCG [CT]^GGCC[GA]
1650 TGGCCA
HaeII 60 GGCGCT [GA]GCGC^[CT]
239 AGCGCT
HincII 220 GTCGAC GT[CT]^[GA]AC
528 GTTGAC
HindII 220 GTCGAC GT[CT]^[GA]AC
528 GTTGAC
KpnI 724 GGTACC GGTAC^C
1550 GGTACC
NciI 156 CCCGG CC^[GC]GG
249 CCGGG
NcoI 647 CCATGG C^CATGG
1400 CCATGG
PleI 85 GACTC GAGTC(4/5)
1353 GACTC
TfiI 121 GAATC G^A[AT]TC
778 GAATC
Tsp45I 1510 GTCAC ^GT[GC]AC
1705 GTGAC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AspHI BanI BbsI BbvI
BfaI BglII BmyI BpuAI BsaJI
BsiHKAI BsmFI Bsp1286I BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII