Restriction map for
pF1KB5016
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# NO CUT ENZYMES ( 130 enzymes) #
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Acc65I AccI AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 199 GACGTC GACGT^C
AocI 1060 CCTGAGG CC^T[ACGT]AGG
ApaI 1056 GGGCCC GGGCC^C
AspHI 1890 GAGCAC G[AT]GC[AT]^C
AvaI 1639 CTCGAG C^[CT]CG[GA]G
BbrPI 1277 CACGTG CAC^GTG
BfaI 1496 CTAG C^TAG
BglI 2021 GCCAAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1277 CACGTG [CT]AC^GT[GA]
BsiHKAI 1890 GAGCAC G[AT]GC[AT]^C
BsmI 669 GCATTC GAATGC(1/-1)
BsoBI 1639 CTCGAG C^[CT]CG[GA]G
BspMI 1463 ACCTGC ACCTGC(4/8)
BsrBI 398 GAGCGG CCGCTC(-3/-3)
BsrDI 1981 GCAATG GCAATG(2/0)
BsrFI 1884 GCCGGT [GA]^CCGG[CT]
Bsu36I 1060 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1884 GCCGGT [GA]^CCGG[CT]
DpnII 647 GATC ^GATC
DraIII 1379 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1060 CCTGAGG CC^T[ACGT]AGG
EcoT14I 336 CCAAGG C^C[AT][AT]GG
FauI 380 GCGGG CCCGC(4/6)
MaeI 1496 CTAG C^TAG
MaeIII 97 GTGAC ^GT[ACGT]AC
MboI 647 GATC ^GATC
MseI 256 TTAA T^TAA
NdeII 647 GATC ^GATC
NspI 657 ACATGC [GA]CATG^[CT]
PaeR7I 1639 CTCGAG C^TCGAG
PmaCI 1277 CACGTG CAC^GTG
PmlI 1277 CACGTG CAC^GTG
PstI 1465 CTGCAG CTGCA^G
Sau3AI 647 GATC ^GATC
SbfI 1464 CCTGCAGG CCTGCA^GG
Sse8387I 1464 CCTGCAGG CCTGCA^GG
StyI 336 CCAAGG C^C[AT][AT]GG
Tru9I 256 TTAA T^TAA
Tsp45I 97 GTGAC ^GT[GC]AC
XhoI 1639 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 1680 GAATTT [GA]^AATT[CT]
2014 AAATTC
AcyI 199 GACGTC G[GA]^CG[CT]C
1048 GGCGTC
Aor51HI 145 AGCGCT AGC^GCT
1079 AGCGCT
ApoI 1680 GAATTT [GA]^AATT[CT]
2014 AAATTC
BalI 641 TGGCCA TGG^CCA
953 TGGCCA
BcnI 665 CCGGG CC^[GC]GG
1237 CCGGG
BsaHI 199 GACGTC G[GA]^CG[CT]C
1048 GGCGTC
BsaI 260 GAGACC GGTCTC(1/5)
1460 GAGACC
BssSI 286 CACGAG CACGAG(-5/-1)
834 CACGAG
CfoI 146 GCGC GCG^C
1080 GCGC
DrdI 433 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1930 GACCAGACAGTC
EaeI 641 TGGCCA [CT]^GGCC[GA]
953 TGGCCA
Eco47III 145 AGCGCT AGC^GCT
1079 AGCGCT
EcoNI 293 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1941 CCTGACAGAGG
HaeII 145 AGCGCT [GA]GCGC^[CT]
1079 AGCGCT
HgaI 830 GACGC GACGC(5/10)
1049 GCGTC
HhaI 146 GCGC GCG^C
1080 GCGC
Hin1I 199 GACGTC G[GA]^CG[CT]C
1048 GGCGTC
HinP1I 146 GCGC G^CGC
1080 GCGC
MluNI 641 TGGCCA TGG^CCA
953 TGGCCA
MscI 641 TGGCCA TGG^CCA
953 TGGCCA
NciI 665 CCGGG CC^[GC]GG
1237 CCGGG
XcmI 809 CCACCTCTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1378 CCACAAGGTGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI Asp700I AvaII BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DraII DsaI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NlaIII NlaIV PleI
PpuMI PvuII SapI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XmnI