Restriction map for
pF1KB4991
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaI BsgI
BsiWI BsmBI BsmI BspDI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MseI MspA1I MunI NaeI NcoI
NdeI NgoMIV NheI NotI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuII RsrII SacI SacII SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI StyI SwaI Tru9I Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 345 GTCGAC GT^[AC][GT]AC
AccIII 872 TCCGGA T^CCGGA
AcsI 310 GAATTC [GA]^AATT[CT]
AcyI 455 GGCGCC G[GA]^CG[CT]C
ApoI 310 GAATTC [GA]^AATT[CT]
Asp700I 576 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 259 GTGCTC G[AT]GC[AT]^C
AvaI 75 CCCGAG C^[CT]CG[GA]G
BalI 591 TGGCCA TGG^CCA
BanI 455 GGCGCC G^G[CT][GA]CC
BbeI 455 GGCGCC GGCGC^C
BclI 701 TGATCA T^GATCA
BglI 790 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 364 TACGTG [CT]AC^GT[GA]
BsaHI 455 GGCGCC G[GA]^CG[CT]C
BseAI 872 TCCGGA T^CCGGA
BsiHKAI 259 GTGCTC G[AT]GC[AT]^C
BsoBI 75 CCCGAG C^[CT]CG[GA]G
Bsp1407I 611 TGTACA T^GTACA
BspEI 872 TCCGGA T^CCGGA
BspHI 704 TCATGA T^CATGA
BspMI 638 ACCTGC ACCTGC(4/8)
BsrBI 84 GAGCGG CCGCTC(-3/-3)
BsrGI 611 TGTACA T^GTACA
BstAPI 772 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 320 GGATCT [GA]^GATC[CT]
DdeI 839 CTAAG C^T[ACGT]AG
DrdI 553 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 269 CCGTGG C^C[GA][CT]GG
EaeI 591 TGGCCA [CT]^GGCC[GA]
EcoRI 310 GAATTC G^AATTC
FbaI 701 TGATCA T^GATCA
Hin1I 455 GGCGCC G[GA]^CG[CT]C
HincII 345 GTCGAC GT[CT]^[GA]AC
HindII 345 GTCGAC GT[CT]^[GA]AC
HinfI 351 GATTC G^A[ACGT]TC
KasI 455 GGCGCC G^GCGCC
MaeII 365 ACGT A^CGT
MflI 320 GGATCT [GA]^GATC[CT]
MluNI 591 TGGCCA TGG^CCA
MroI 872 TCCGGA T^CCGGA
MscI 591 TGGCCA TGG^CCA
MslI 754 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 455 GGCGCC GG^CGCC
NruI 293 TCGCGA TCG^CGA
PvuI 26 CGATCG CGAT^CG
RcaI 704 TCATGA T^CATGA
SalI 345 GTCGAC G^TCGAC
SbfI 639 CCTGCAGG CCTGCA^GG
SfoI 455 GGCGCC GGC^GCC
Sse8387I 639 CCTGCAGG CCTGCA^GG
SspBI 611 TGTACA T^GTACA
TfiI 351 GATTC G^A[AT]TC
Tsp509I 311 AATT ^AATT
XcmI 758 CCAGTGATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 320 GGATCT [GA]^GATC[CT]
XmnI 576 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 275 GGATC GGATC(4/5)
320 GGATC
BanII 665 GAGCCC G[GA]GC[CT]^C
788 GAGCCC
BcnI 33 CCCGG CC^[GC]GG
57 CCCGG
BsaWI 317 ACCGGA [AT]^CCGG[AT]
872 TCCGGA
BsiEI 26 CGATCG CG[GA][CT]^CG
343 CGGTCG
BsiYI 129 CCTCTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
360 CCCCTACGTGG
BslI 129 CCTCTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
360 CCCCTACGTGG
BsmFI 723 GGGAC GGGAC(10/14)
31 GTCCC
BstF5I 288 CATCC GGATG(2/0)
754 CATCC
CfoI 61 GCGC GCG^C
456 GCGC
FokI 288 CATCC GGATG(9/13)
754 CATCC
HaeII 60 GGCGCT [GA]GCGC^[CT]
455 GGCGCC
HhaI 61 GCGC GCG^C
456 GCGC
HinP1I 61 GCGC G^CGC
456 GCGC
NciI 33 CCCGG CC^[GC]GG
57 CCCGG
PstI 640 CTGCAG CTGCA^G
688 CTGCAG
SfaNI 539 GATGC GCATC(5/9)
215 GCATC
SmlI 474 CTTGAG C^T[CT][GA]AG
782 CTCAAG
TspRI 227 CACTG CA[GC]TG[ACGT][ACGT]^
759 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI BbvI BmyI
BsaJI BseRI BsmAI Bsp1286I BsrDI
BsrI BssKI BstNI BstUI Cac8I
Cfr13I DpnII EarI EcoRII FauI
Fnu4HI HaeIII HapII HgaI HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
TthHB8I