Restriction map for
pF1KB4969
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AviII AvrII BbeI BlnI
BsaBI BsaHI BsgI BsiEI BsiWI
BsmBI BspDI BsrBI BsrFI BssHII
Bst1107I BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII KasI KspI MamI MluI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SpeI Sse8387I StuI
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 2704 TCCGGA T^CCGGA
AocI 1391 CCTCAGG CC^T[ACGT]AGG
AseI 5422 ATTAAT AT^TAAT
AsnI 5422 ATTAAT AT^TAAT
AspI 1816 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2282 GCACTGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4344 CCCGG CC^[GC]GG
BglI 5170 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2587 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2587 GCTAAGC GC^T[ACGT]AGC
BsaWI 2704 TCCGGA [AT]^CCGG[AT]
BseAI 2704 TCCGGA T^CCGGA
BspEI 2704 TCCGGA T^CCGGA
BspLU11I 6558 ACATGT A^CATGT
BspMI 5486 ACCTGC ACCTGC(4/8)
BssSI 1019 CTCGTG CACGAG(-5/-1)
BstBI 4859 TTCGAA TT^CGAA
BstEII 3576 GGTGACC G^GT[ACGT]ACC
BstPI 3576 GGTGACC G^GT[ACGT]ACC
Bsu36I 1391 CCTCAGG CC^T[ACGT]AGG
CelII 2587 GCTAAGC GC^T[ACGT]AGC
DraIII 2552 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1391 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3576 GGTGACC G^GT[ACGT]ACC
EcoRV 4359 GATATC GAT^ATC
EcoT22I 2125 ATGCAT ATGCA^T
HgaI 418 GACGC GACGC(5/10)
MfeI 2907 CAATTG C^AATTG
MroI 2704 TCCGGA T^CCGGA
MunI 2907 CAATTG C^AATTG
NciI 4344 CCCGG CC^[GC]GG
NsiI 2125 ATGCAT ATGCA^T
NspV 4859 TTCGAA TT^CGAA
PflFI 1816 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5422 ATTAAT AT^TAAT
SfuI 4859 TTCGAA TT^CGAA
SnaBI 5122 TACGTA TAC^GTA
SphI 5130 GCATGC GCATG^C
SspI 2344 AATATT AAT^ATT
Tth111I 1816 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 904 CCAGAGGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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Acc65I 1670 GGTACC G^GTACC
4709 GGTACC
AflII 1006 CTTAAG C^TTAAG
3190 CTTAAG
AflIII 6321 ACGTGT A^C[GA][CT]GT
6558 ACATGT
AlwNI 820 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
5712 CAGAGACTG
ApaI 562 GGGCCC GGGCC^C
900 GGGCCC
Asp718I 1670 GGTACC G^GTACC
4709 GGTACC
BalI 1313 TGGCCA TGG^CCA
2988 TGGCCA
BbrPI 2936 CACGTG CAC^GTG
5518 CACGTG
BciVI 2537 GGATAC GTATCC(6/5)
2051 GTATCC
BfrI 1006 CTTAAG C^TTAAG
3190 CTTAAG
BsaI 846 GGTCTC GGTCTC(1/5)
1127 GGTCTC
BseRI 4367 CTCCTC GAGGAG(10/8)
4677 CTCCTC
BspHI 1532 TCATGA T^CATGA
1754 TCATGA
BstAPI 627 GCAAATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5863 GCAGAATTTGC
DsaI 5016 CCATGG C^C[GA][CT]GG
6515 CCATGG
HapII 2705 CCGG C^CGG
4345 CCGG
HpaI 1986 GTTAAC GTT^AAC
2902 GTTAAC
HpaII 2705 CCGG C^CGG
4345 CCGG
KpnI 1670 GGTACC GGTAC^C
4709 GGTACC
MluNI 1313 TGGCCA TGG^CCA
2988 TGGCCA
MscI 1313 TGGCCA TGG^CCA
2988 TGGCCA
MspI 2705 CCGG C^CGG
4345 CCGG
NcoI 5016 CCATGG C^CATGG
6515 CCATGG
NdeI 2075 CATATG CA^TATG
3898 CATATG
PaeR7I 4898 CTCGAG C^TCGAG
6137 CTCGAG
PmaCI 2936 CACGTG CAC^GTG
5518 CACGTG
PmlI 2936 CACGTG CAC^GTG
5518 CACGTG
PstI 1064 CTGCAG CTGCA^G
2192 CTGCAG
RcaI 1532 TCATGA T^CATGA
1754 TCATGA
SacI 780 GAGCTC GAGCT^C
3454 GAGCTC
SapI 4912 GAAGAGC GCTCTTC(1/4)
5337 GAAGAGC
XhoI 4898 CTCGAG C^TCGAG
6137 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BclI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII XmnI