Restriction map for
pF1KB4920
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AlwNI AocI
Aor51HI ApaI AscI Asp718I AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I CpoI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV FauI FbaI FseI
FspI HaeII HapII Hin1I HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflII 223 CTTAAG C^TTAAG
AflIII 2253 ACGTGT A^C[GA][CT]GT
AhdI 1545 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 913 GATCC GGATC(4/5)
AseI 1486 ATTAAT AT^TAAT
AsnI 1486 ATTAAT AT^TAAT
AspEI 1545 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 2266 GGTGCC G^G[CT][GA]CC
BanII 214 GAGCTC G[GA]GC[CT]^C
BcgI 211 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 336 GTATCC GTATCC(6/5)
BfrI 223 CTTAAG C^TTAAG
BseRI 404 CTCCTC GAGGAG(10/8)
BsgI 568 GTGCAG GTGCAG(16/14)
BsiYI 171 CCCAGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 171 CCCAGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI 504 ATCGAT AT^CGAT
BstEII 492 GGTGACC G^GT[ACGT]ACC
BstPI 492 GGTGACC G^GT[ACGT]ACC
BstXI 1278 CCACCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1979 GCGC GCG^C
ClaI 504 ATCGAT AT^CGAT
DpnII 913 GATC ^GATC
DraI 1942 TTTAAA TTT^AAA
DraII 702 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 673 CCATGG C^C[GA][CT]GG
Eam1105I 1545 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 394 GAAGAG CTCTTC(1/4)
EcoO109I 702 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 492 GGTGACC G^GT[ACGT]ACC
EcoT14I 673 CCATGG C^C[AT][AT]GG
EcoT22I 2195 ATGCAT ATGCA^T
HaeIII 703 GGCC GG^CC
HgaI 561 GCGTC GACGC(5/10)
HhaI 1979 GCGC GCG^C
HinP1I 1979 GCGC G^CGC
HindIII 2048 AAGCTT A^AGCTT
Ksp632I 394 GAAGAG CTCTTC(1/4)
MboI 913 GATC ^GATC
NcoI 673 CCATGG C^CATGG
NdeII 913 GATC ^GATC
NsiI 2195 ATGCAT ATGCA^T
PshAI 1378 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1486 ATTAAT AT^TAAT
SacI 214 GAGCTC GAGCT^C
SalI 143 GTCGAC G^TCGAC
Sau3AI 913 GATC ^GATC
ScaI 1642 AGTACT AGT^ACT
StyI 673 CCATGG C^C[AT][AT]GG
XbaI 390 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 143 GTCGAC GT^[AC][GT]AC
529 GTAGAC
AvaII 82 GGACC G^G[AT]CC
184 GGTCC
BbvI 347 GCAGC GCAGC(8/12)
270 GCTGC
BsaJI 539 CCCAGG C^C[ACGT][ACGT]GG
673 CCATGG
BsmFI 627 GGGAC GGGAC(10/14)
330 GTCCC
BsrDI 1529 GCAATG GCAATG(2/0)
1613 GCAATG
Fnu4HI 270 GCTGC GC^[ACGT]GC
347 GCAGC
HincII 143 GTCGAC GT[CT]^[GA]AC
203 GTCAAC
HindII 143 GTCGAC GT[CT]^[GA]AC
203 GTCAAC
ItaI 270 GCTGC GC^[ACGT]GC
347 GCAGC
MwoI 270 GCTGCTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
561 GCGTCCTGTGC
NlaIV 82 GGACCC GG[ACGT]^[ACGT]CC
2266 GGTGCC
SfcI 527 CTGTAG C^T[GA][CT]AG
917 CTACAG
SmlI 9 CTCAAG C^T[CT][GA]AG
223 CTTAAG
TseI 270 GCTGC G^C[AT]GC
347 GCAGC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI Alw44I ApaLI
ApoI Asp700I AspHI BbsI BfaI
BmyI BpmI BpuAI BsaI BsiHKAI
BsmAI BsmI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRI EcoRII FokI
HinfI HphI MaeI MaeII MaeIII
MboII MnlI MseI MslI MvaI
NdeI NlaIII NspI PleI RsaI
Sau96I ScrFI SfaNI SspBI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XmnI