Restriction map for
pF1KB4902
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BbvCI BciVI BclI
BcnI BglI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1270 GTAGAC GT^[AC][GT]AC
AflII 2196 CTTAAG C^TTAAG
AflIII 753 ACATGT A^C[GA][CT]GT
AlwNI 1057 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1903 CCTCAGG CC^T[ACGT]AGG
Asp700I 358 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 93 TGGCCA TGG^CCA
BanI 1896 GGCACC G^G[CT][GA]CC
BanII 841 GAGCCC G[GA]GC[CT]^C
BbrPI 1996 CACGTG CAC^GTG
BcgI 562 GCAGAAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2196 CTTAAG C^TTAAG
BpmI 722 CTCCAG CTGGAG(16/14)
BsaAI 1996 CACGTG [CT]AC^GT[GA]
BsaBI 2112 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1294 GAGACC GGTCTC(1/5)
BspHI 1038 TCATGA T^CATGA
BspLU11I 753 ACATGT A^CATGT
BstAPI 1660 GCAAAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1531 GGTGACC G^GT[ACGT]ACC
BstPI 1531 GGTGACC G^GT[ACGT]ACC
Bsu36I 1903 CCTCAGG CC^T[ACGT]AGG
DrdI 1042 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1903 CCTCAGG CC^T[ACGT]AGG
EcoNI 490 CCTGCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1531 GGTGACC G^GT[ACGT]ACC
EcoRV 1459 GATATC GAT^ATC
FauI 4 GCGGG CCCGC(4/6)
HapII 9 CCGG C^CGG
HincII 1051 GTCAAC GT[CT]^[GA]AC
HindII 1051 GTCAAC GT[CT]^[GA]AC
HpaII 9 CCGG C^CGG
MamI 2112 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MspA1I 2023 CAGCTG C[AC]G^C[GT]G
MspI 9 CCGG C^CGG
NcoI 461 CCATGG C^CATGG
NheI 2212 GCTAGC G^CTAGC
PaeR7I 1544 CTCGAG C^TCGAG
PmaCI 1996 CACGTG CAC^GTG
PmlI 1996 CACGTG CAC^GTG
PpuMI 112 AGGACCT [GA]G^G[AT]CC[CT]
PstI 304 CTGCAG CTGCA^G
PvuII 2023 CAGCTG CAG^CTG
RcaI 1038 TCATGA T^CATGA
ScaI 1378 AGTACT AGT^ACT
SphI 14 GCATGC GCATG^C
StuI 383 AGGCCT AGG^CCT
XcmI 1376 CCAGTACTAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1544 CTCGAG C^TCGAG
XmnI 358 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 710 CCCGAG C^[CT]CG[GA]G
1544 CTCGAG
BamHI 465 GGATCC G^GATCC
2160 GGATCC
BmyI 841 GAGCCC G[AGT]GC[ACT]^C
1999 GTGCCC
BsgI 62 GTGCAG GTGCAG(16/14)
1573 GTGCAG
BsmI 831 GAATGC GAATGC(1/-1)
181 GCATTC
BsoBI 710 CCCGAG C^[CT]CG[GA]G
1544 CTCGAG
Bsp1286I 841 GAGCCC G[AGT]GC[ACT]^C
1999 GTGCCC
BsrDI 177 CATTGC GCAATG(2/0)
2007 CATTGC
DraII 112 AGGACCT [GA]G^G[ACGT]CC[CT]
1626 AGGCCCT
DsaI 461 CCATGG C^C[GA][CT]GG
1289 CCGTGG
EaeI 93 TGGCCA [CT]^GGCC[GA]
1286 TGGCCG
EcoO109I 112 AGGACCT [GA]G^G[ACGT]CC[CT]
1626 AGGCCCT
EcoT14I 461 CCATGG C^C[AT][AT]GG
602 CCAAGG
HphI 444 GGTGA GGTGA(8/7)
1531 GGTGA
MaeII 1832 ACGT A^CGT
1997 ACGT
MfeI 57 CAATTG C^AATTG
2078 CAATTG
MunI 57 CAATTG C^AATTG
2078 CAATTG
PflMI 784 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1468 CCAGCCTTTGG
PleI 322 GACTC GAGTC(4/5)
798 GACTC
SapI 1144 GCTCTTC GCTCTTC(1/4)
1873 GCTCTTC
SmlI 1544 CTCGAG C^T[CT][GA]AG
2196 CTTAAG
StyI 461 CCATGG C^C[AT][AT]GG
602 CCAAGG
Van91I 784 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1468 CCAGCCTTTGG
XbaI 666 TCTAGA T^CTAGA
1551 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BglII BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HindIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII