Restriction map for
pF1KB4846
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwNI ApoI
AscI AseI AsnI Asp718I AspEI
AspI BalI BbrPI BfrI BglI
BglII BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmFI Bsp1407I BspDI
BspEI BspLU11I BsrGI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoRI EcoRV
FseI HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI ScaI SfiI SfuI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 88 GACGTC GACGT^C
AccI 956 GTCGAC GT^[AC][GT]AC
Alw44I 886 GTGCAC G^TGCAC
AocI 480 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1265 AGCGCT AGC^GCT
ApaI 595 GGGCCC GGGCC^C
ApaLI 886 GTGCAC G^TGCAC
Asp700I 510 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 886 GTGCAC G[AT]GC[AT]^C
AvaII 836 GGTCC G^G[AT]CC
AviII 269 TGCGCA TGC^GCA
AvrII 360 CCTAGG C^CTAGG
BamHI 865 GGATCC G^GATCC
BcgI 1088 GCATCGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 804 GGATAC GTATCC(6/5)
BclI 633 TGATCA T^GATCA
BlnI 360 CCTAGG C^CTAGG
BsgI 1293 CTGCAC GTGCAG(16/14)
BsiHKAI 886 GTGCAC G[AT]GC[AT]^C
BsmAI 86 GAGAC GTCTC(1/5)
BsmBI 86 GAGACG CGTCTC(1/5)
BsmI 58 GAATGC GAATGC(1/-1)
BspHI 50 TCATGA T^CATGA
BspMI 1139 ACCTGC ACCTGC(4/8)
BssHII 1408 GCGCGC G^CGCGC
BstEII 1077 GGTCACC G^GT[ACGT]ACC
BstPI 1077 GGTCACC G^GT[ACGT]ACC
BstXI 1388 CCACCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 480 CCTCAGG CC^T[ACGT]AGG
EagI 1229 CGGCCG C^GGCCG
EarI 503 GAAGAG CTCTTC(1/4)
EclXI 1229 CGGCCG C^GGCCG
Eco47III 1265 AGCGCT AGC^GCT
Eco52I 1229 CGGCCG C^GGCCG
Eco81I 480 CCTCAGG CC^T[ACGT]AGG
EcoNI 1413 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1077 GGTCACC G^GT[ACGT]ACC
EcoT22I 60 ATGCAT ATGCA^T
FbaI 633 TGATCA T^GATCA
FspI 269 TGCGCA TGC^GCA
HincII 956 GTCGAC GT[CT]^[GA]AC
HindII 956 GTCGAC GT[CT]^[GA]AC
HindIII 513 AAGCTT A^AGCTT
Ksp632I 503 GAAGAG CTCTTC(1/4)
NsiI 60 ATGCAT ATGCA^T
PflMI 855 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1071 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 941 CTGCAG CTGCA^G
PvuII 688 CAGCTG CAG^CTG
RcaI 50 TCATGA T^CATGA
SalI 956 GTCGAC G^TCGAC
SexAI 458 ACCAGGT A^CC[AT]GGT
SfcI 941 CTGCAG C^T[GA][CT]AG
SgrAI 186 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1037 GCATGC GCATG^C
TfiI 75 GAATC G^A[AT]TC
Van91I 855 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 82 TCTAGA T^CTAGA
XcmI 823 CCATCCCCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 510 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 36 GTAC GT^AC
1215 GTAC
BbsI 611 GAAGAC GAAGAC(2/6)
535 GTCTTC
BbvCI 781 CCTCAGC CCTCAGC(-5/-2)
210 GCTGAGG
BlpI 138 GCTGAGC GC^T[ACGT]AGC
424 GCTGAGC
BpmI 682 CTGGAG CTGGAG(16/14)
1414 CTGGAG
Bpu1102I 138 GCTGAGC GC^T[ACGT]AGC
424 GCTGAGC
BpuAI 611 GAAGAC GAAGAC(2/6)
535 GTCTTC
BseRI 213 GAGGAG GAGGAG(10/8)
1417 GAGGAG
BsrBI 216 GAGCGG CCGCTC(-3/-3)
667 GAGCGG
BsrDI 870 CATTGC GCAATG(2/0)
404 GCAATG
BssSI 105 CACGAG CACGAG(-5/-1)
746 CTCGTG
BstYI 815 AGATCC [GA]^GATC[CT]
865 GGATCC
CelII 138 GCTGAGC GC^T[ACGT]AGC
424 GCTGAGC
DraII 595 GGGCCCC [GA]G^G[ACGT]CC[CT]
1432 AGGCCCT
EcoO109I 595 GGGCCCC [GA]G^G[ACGT]CC[CT]
1432 AGGCCCT
MaeIII 388 GTCAC ^GT[ACGT]AC
1078 GTCAC
MflI 815 AGATCC [GA]^GATC[CT]
865 GGATCC
MseI 473 TTAA T^TAA
776 TTAA
NcoI 1109 CCATGG C^CATGG
1250 CCATGG
NspI 1037 GCATGC [GA]CATG^[CT]
1100 GCATGT
RsaI 36 GTAC GT^AC
1215 GTAC
SmaI 255 CCCGGG CCC^GGG
646 CCCGGG
Tru9I 473 TTAA T^TAA
776 TTAA
Tsp45I 388 GTCAC ^GT[GC]AC
1078 GTCAC
XhoII 815 AGATCC [GA]^GATC[CT]
865 GGATCC
XmaCI 255 CCCGGG C^CCGGG
646 CCCGGG
XmaI 255 CCCGGG C^CCGGG
646 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI BanI BanII BbeI BbvI
BcnI BfaI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfoI
StyI TaqI TseI TspRI TthHB8I