Restriction map for
pF1KB4796
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 589 GGTACC G^GTACC
AcyI 49 GACGCC G[GA]^CG[CT]C
AlwI 368 GGATC GGATC(4/5)
AseI 469 ATTAAT AT^TAAT
AsnI 469 ATTAAT AT^TAAT
Asp718I 589 GGTACC G^GTACC
AvaII 62 GGACC G^G[AT]CC
BanI 589 GGTACC G^G[CT][GA]CC
BbsI 46 GAAGAC GAAGAC(2/6)
BbvI 382 GCAGC GCAGC(8/12)
BfaI 523 CTAG C^TAG
BpuAI 46 GAAGAC GAAGAC(2/6)
BsaHI 49 GACGCC G[GA]^CG[CT]C
BsaJI 53 CCGAGG C^C[ACGT][ACGT]GG
BseRI 55 GAGGAG GAGGAG(10/8)
BsmAI 75 GAGAC GTCTC(1/5)
BsmBI 75 GAGACG CGTCTC(1/5)
BsrI 271 CCAGT ACTGG(1/-1)
BstF5I 627 GGATG GGATG(2/0)
BstXI 641 CCATGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 368 GGATCT [GA]^GATC[CT]
Cac8I 140 GCAAGC GC[ACGT]^[ACGT]GC
DpnII 369 GATC ^GATC
EcoT22I 183 ATGCAT ATGCA^T
Fnu4HI 382 GCAGC GC^[ACGT]GC
FokI 627 GGATG GGATG(9/13)
HaeIII 115 GGCC GG^CC
HgaI 49 GACGC GACGC(5/10)
Hin1I 49 GACGCC G[GA]^CG[CT]C
HphI 550 TCACC GGTGA(8/7)
ItaI 382 GCAGC GC^[ACGT]GC
KpnI 589 GGTACC GGTAC^C
MaeI 523 CTAG C^TAG
MaeIII 549 GTCAC ^GT[ACGT]AC
MboI 369 GATC ^GATC
MflI 368 GGATCT [GA]^GATC[CT]
NdeII 369 GATC ^GATC
NsiI 183 ATGCAT ATGCA^T
PshBI 469 ATTAAT AT^TAAT
Sau3AI 369 GATC ^GATC
SfcI 154 CTACAG C^T[GA][CT]AG
SspI 196 AATATT AAT^ATT
TseI 382 GCAGC G^C[AT]GC
Tsp45I 549 GTCAC ^GT[GC]AC
TspRI 37 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 368 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 92 AAATTT [GA]^AATT[CT]
310 GAATTT
ApoI 92 AAATTT [GA]^AATT[CT]
310 GAATTT
BsgI 656 CTGCAC GTGCAG(16/14)
380 GTGCAG
BsiYI 53 CCGAGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
475 CCTAATGCTGG
BslI 53 CCGAGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
475 CCTAATGCTGG
BssKI 100 CCTGG ^CC[ACGT]GG
252 CCTGG
BstNI 100 CCTGG CC^[AT]GG
252 CCTGG
Cfr13I 62 GGACC G^G[ACGT]CC
115 GGCCC
DdeI 111 CTTAG C^T[ACGT]AG
305 CTAAG
EarI 606 GAAGAG CTCTTC(1/4)
622 GAAGAG
EcoRII 100 CCTGG ^CC[AT]GG
252 CCTGG
Ksp632I 606 GAAGAG CTCTTC(1/4)
622 GAAGAG
MaeII 8 ACGT A^CGT
288 ACGT
MslI 355 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
642 CATGCAAGTG
MvaI 100 CCTGG CC^[AT]GG
252 CCTGG
NlaIV 589 GGTACC GG[ACGT]^[ACGT]CC
637 GGAACC
Sau96I 62 GGACC G^G[ACGT]CC
115 GGCCC
ScrFI 100 CCTGG CC^[ACGT]GG
252 CCTGG
SfaNI 84 GATGC GCATC(5/9)
433 GATGC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AfaI AluI BpmI MboII MnlI
MseI NlaIII RsaI Tru9I Tsp509I