##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
AlwNI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsiWI Bsp1407I BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MaeI MfeI MluI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2750 GACGTC GACGT^C
Acc65I 137 GGTACC G^GTACC
AccIII 3734 TCCGGA T^CCGGA
AflIII 152 ACGTGT A^C[GA][CT]GT
Alw44I 581 GTGCAC G^TGCAC
ApaLI 581 GTGCAC G^TGCAC
Asp718I 137 GGTACC G^GTACC
BalI 4043 TGGCCA TGG^CCA
BamHI 2803 GGATCC G^GATCC
BsaBI 4185 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3734 TCCGGA T^CCGGA
BsiEI 3504 CGGTCG CG[GA][CT]^CG
BsmI 2183 GAATGC GAATGC(1/-1)
BspDI 4318 ATCGAT AT^CGAT
BspEI 3734 TCCGGA T^CCGGA
BstXI 4134 CCAGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 4318 ATCGAT AT^CGAT
DraIII 1709 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 3807 GAAGAG CTCTTC(1/4)
EcoNI 4149 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4357 GAATTC G^AATTC
KpnI 137 GGTACC GGTAC^C
Ksp632I 3807 GAAGAG CTCTTC(1/4)
MamI 4185 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4043 TGGCCA TGG^CCA
MroI 3734 TCCGGA T^CCGGA
MscI 4043 TGGCCA TGG^CCA
NspI 3966 GCATGT [GA]CATG^[CT]
ScaI 3818 AGTACT AGT^ACT
SfcI 4221 CTACAG C^T[GA][CT]AG
SgrAI 321 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2845 CCCGGG CCC^GGG
StuI 4082 AGGCCT AGG^CCT
XmaCI 2845 CCCGGG C^CCGGG
XmaI 2845 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 521 GAATTT [GA]^AATT[CT]
4357 GAATTC
Aor51HI 2599 AGCGCT AGC^GCT
3577 AGCGCT
ApoI 521 GAATTT [GA]^AATT[CT]
4357 GAATTC
BsgI 4242 CTGCAC GTGCAG(16/14)
2201 GTGCAG
BsmBI 149 GAGACG CGTCTC(1/5)
3077 GAGACG
BstEII 3100 GGTGACC G^GT[ACGT]ACC
3325 GGTCACC
BstPI 3100 GGTGACC G^GT[ACGT]ACC
3325 GGTCACC
DrdI 3461 GACTCAACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3551 GACCTCCTGGTC
Eco47III 2599 AGCGCT AGC^GCT
3577 AGCGCT
EcoO65I 3100 GGTGACC G^GT[ACGT]ACC
3325 GGTCACC
MslI 3628 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4317 CATCGATGTG
Tsp509I 522 AATT ^AATT
4358 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PaeR7I PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfiI
SfoI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoI XhoII