Restriction map for
pF1KB4626
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BcgI BciVI
BfrI BlnI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AspI 289 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1217 TGCGCA TGC^GCA
BalI 806 TGGCCA TGG^CCA
BamHI 1691 GGATCC G^GATCC
BbrPI 514 CACGTG CAC^GTG
BclI 1055 TGATCA T^GATCA
BglII 760 AGATCT A^GATCT
BlpI 837 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 837 GCTCAGC GC^T[ACGT]AGC
BsaAI 514 CACGTG [CT]AC^GT[GA]
BsaI 1925 GAGACC GGTCTC(1/5)
BsiEI 264 CGGCCG CG[GA][CT]^CG
BspHI 1355 TCATGA T^CATGA
BspLU11I 442 ACATGT A^CATGT
BssSI 1776 CACGAG CACGAG(-5/-1)
CelII 837 GCTCAGC GC^T[ACGT]AGC
EagI 264 CGGCCG C^GGCCG
EclXI 264 CGGCCG C^GGCCG
Eco52I 264 CGGCCG C^GGCCG
FbaI 1055 TGATCA T^GATCA
FspI 1217 TGCGCA TGC^GCA
HpaI 976 GTTAAC GTT^AAC
MluNI 806 TGGCCA TGG^CCA
MscI 806 TGGCCA TGG^CCA
NaeI 266 GCCGGC GCC^GGC
NgoMIV 266 GCCGGC G^CCGGC
PflFI 289 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 514 CACGTG CAC^GTG
PmlI 514 CACGTG CAC^GTG
PpuMI 1838 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1355 TCATGA T^CATGA
SgrAI 1224 CACCGGCG C[GA]^CCGG[CT]G
StuI 1541 AGGCCT AGG^CCT
TfiI 1002 GAATC G^A[AT]TC
Tsp509I 803 AATT ^AATT
Tth111I 289 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1872 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccIII 1388 TCCGGA T^CCGGA
1694 TCCGGA
AfaI 330 GTAC GT^AC
1302 GTAC
AflIII 442 ACATGT A^C[GA][CT]GT
872 ACGTGT
ApaI 1247 GGGCCC GGGCC^C
1587 GGGCCC
BbvCI 396 CCTCAGC CCTCAGC(-5/-2)
1712 CCTCAGC
BglI 619 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1446 GCCTCCAGGGC
BseAI 1388 TCCGGA T^CCGGA
1694 TCCGGA
BseRI 1263 CTCCTC GAGGAG(10/8)
1734 CTCCTC
BspEI 1388 TCCGGA T^CCGGA
1694 TCCGGA
BsrBI 20 GAGCGG CCGCTC(-3/-3)
261 GAGCGG
BstAPI 183 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
231 GCACTCCCTGC
BstEII 1980 GGTCACC G^GT[ACGT]ACC
2280 GGTCACC
BstPI 1980 GGTCACC G^GT[ACGT]ACC
2280 GGTCACC
BstXI 1794 CCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2270 CCAAGCAGCTGG
EcoO65I 1980 GGTCACC G^GT[ACGT]ACC
2280 GGTCACC
HincII 976 GTTAAC GT[CT]^[GA]AC
1363 GTCAAC
HindII 976 GTTAAC GT[CT]^[GA]AC
1363 GTCAAC
HindIII 355 AAGCTT A^AGCTT
1783 AAGCTT
MroI 1388 TCCGGA T^CCGGA
1694 TCCGGA
NcoI 866 CCATGG C^CATGG
1502 CCATGG
NspI 442 ACATGT [GA]CATG^[CT]
776 GCATGT
PaeR7I 414 CTCGAG C^TCGAG
1126 CTCGAG
PflMI 333 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
636 CCATGAGCTGG
RsaI 330 GTAC GT^AC
1302 GTAC
SmlI 414 CTCGAG C^T[CT][GA]AG
1126 CTCGAG
Van91I 333 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
636 CCATGAGCTGG
XhoI 414 CTCGAG C^TCGAG
1126 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AluI Alw44I
AlwI AlwNI ApaLI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII