Restriction map for
pF1KB4618
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AhdI AocI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbeI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BseAI
BseRI BsgI BsiWI BspEI BspHI
BspMI BssHII BssSI BstAPI BstBI
BstUI Bsu36I CelII CpoI DraI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1905 GTATAC GT^[AC][GT]AC
AcyI 439 GGCGTC G[GA]^CG[CT]C
AgeI 1766 ACCGGT A^CCGGT
Alw44I 2052 GTGCAC G^TGCAC
AlwNI 1676 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 977 AGCGCT AGC^GCT
ApaI 710 GGGCCC GGGCC^C
ApaLI 2052 GTGCAC G^TGCAC
AviII 578 TGCGCA TGC^GCA
BbrPI 348 CACGTG CAC^GTG
BbvCI 796 GCTGAGG CCTCAGC(-5/-2)
BglI 712 GCCCTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 358 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 439 GGCGTC G[GA]^CG[CT]C
BsaI 862 GGTCTC GGTCTC(1/5)
BsiEI 1473 CGACCG CG[GA][CT]^CG
BsmBI 441 CGTCTC CGTCTC(1/5)
BspDI 553 ATCGAT AT^CGAT
BspLU11I 653 ACATGT A^CATGT
BsrBI 319 GAGCGG CCGCTC(-3/-3)
Bst1107I 1905 GTATAC GTA^TAC
BstXI 1298 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1905 GTATAC GTA^TAC
ClaI 553 ATCGAT AT^CGAT
DraIII 2089 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 433 GACTTTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 977 AGCGCT AGC^GCT
EcoNI 948 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 578 TGCGCA TGC^GCA
HaeII 977 AGCGCT [GA]GCGC^[CT]
Hin1I 439 GGCGTC G[GA]^CG[CT]C
MamI 358 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1856 CCATGG C^CATGG
PinAI 1766 ACCGGT A^CCGGT
PmaCI 348 CACGTG CAC^GTG
PmlI 348 CACGTG CAC^GTG
PstI 1315 CTGCAG CTGCA^G
PvuII 794 CAGCTG CAG^CTG
SacI 1052 GAGCTC GAGCT^C
ScaI 927 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1756 GAATTT [GA]^AATT[CT]
2035 AAATTC
ApoI 1756 GAATTT [GA]^AATT[CT]
2035 AAATTC
Asp700I 1678 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2211 GAAGAGCTTC
AspI 821 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2173 GACCCTGTC
AvaI 464 CCCGGG C^[CT]CG[GA]G
1782 CCCGGG
BanI 6 GGTGCC G^G[CT][GA]CC
1518 GGTGCC
BanII 710 GGGCCC G[GA]GC[CT]^C
1052 GAGCTC
BsaWI 1331 ACCGGA [AT]^CCGG[AT]
1766 ACCGGT
BsmI 341 GAATGC GAATGC(1/-1)
770 GCATTC
BsoBI 464 CCCGGG C^[CT]CG[GA]G
1782 CCCGGG
Bsp1407I 1202 TGTACA T^GTACA
2183 TGTACA
BsrGI 1202 TGTACA T^GTACA
2183 TGTACA
BstEII 1777 GGTTACC G^GT[ACGT]ACC
2019 GGTAACC
BstPI 1777 GGTTACC G^GT[ACGT]ACC
2019 GGTAACC
Cac8I 297 GCCAGC GC[ACGT]^[ACGT]GC
1093 GCTGGC
EcoO65I 1777 GGTTACC G^GT[ACGT]ACC
2019 GGTAACC
EcoRV 1702 GATATC GAT^ATC
1927 GATATC
EcoT14I 1514 CCAAGG C^C[AT][AT]GG
1856 CCATGG
HgaI 91 GCGTC GACGC(5/10)
440 GCGTC
MspA1I 794 CAGCTG C[AC]G^C[GT]G
1423 CCGCTG
NdeI 1343 CATATG CA^TATG
1951 CATATG
NspI 206 GCATGT [GA]CATG^[CT]
653 ACATGT
PflFI 821 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2173 GACCCTGTC
SapI 2211 GAAGAGC GCTCTTC(1/4)
14 GCTCTTC
SexAI 822 ACCAGGT A^CC[AT]GGT
992 ACCAGGT
SmaI 464 CCCGGG CCC^GGG
1782 CCCGGG
SmlI 1442 CTCAAG C^T[CT][GA]AG
2149 CTCAAG
SspBI 1202 TGTACA T^GTACA
2183 TGTACA
StyI 1514 CCAAGG C^C[AT][AT]GG
1856 CCATGG
Tsp45I 503 GTGAC ^GT[GC]AC
1996 GTCAC
Tth111I 821 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2173 GACCCTGTC
XmaCI 464 CCCGGG C^CCGGG
1782 CCCGGG
XmaI 464 CCCGGG C^CCGGG
1782 CCCGGG
XmnI 1678 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2211 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BalI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI XhoII