Restriction map for
pF1KB4582
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FseI HgaI
Hin1I HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI NarI NciI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1337 GTCGAC GT^[AC][GT]AC
Asp700I 1671 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1479 TGCGCA TGC^GCA
BalI 795 TGGCCA TGG^CCA
BanII 971 GGGCTC G[GA]GC[CT]^C
BciVI 1309 GGATAC GTATCC(6/5)
BclI 804 TGATCA T^GATCA
BsaI 1149 GAGACC GGTCTC(1/5)
BseRI 578 GAGGAG GAGGAG(10/8)
BsrBI 435 CCGCTC CCGCTC(-3/-3)
BssHII 11 GCGCGC G^CGCGC
DraI 1866 TTTAAA TTT^AAA
DraII 842 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 309 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 692 GACAGTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 727 GAAGAG CTCTTC(1/4)
EcoO109I 842 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 370 GAATTC G^AATTC
EcoT22I 810 ATGCAT ATGCA^T
FbaI 804 TGATCA T^GATCA
FspI 1479 TGCGCA TGC^GCA
HaeII 532 GGCGCT [GA]GCGC^[CT]
HincII 1337 GTCGAC GT[CT]^[GA]AC
HindII 1337 GTCGAC GT[CT]^[GA]AC
Ksp632I 727 GAAGAG CTCTTC(1/4)
MluNI 795 TGGCCA TGG^CCA
MscI 795 TGGCCA TGG^CCA
MslI 39 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 414 GCCGGC GCC^GGC
NdeI 1574 CATATG CA^TATG
NgoMIV 414 GCCGGC G^CCGGC
NsiI 810 ATGCAT ATGCA^T
NspI 1008 GCATGC [GA]CATG^[CT]
PflMI 568 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 286 GACTC GAGTC(4/5)
PshAI 1429 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1337 GTCGAC G^TCGAC
SapI 727 GAAGAGC GCTCTTC(1/4)
ScaI 473 AGTACT AGT^ACT
SfcI 189 CTACAG C^T[GA][CT]AG
SphI 1008 GCATGC GCATG^C
SspI 1568 AATATT AAT^ATT
Van91I 568 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1671 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 117 AAATTT [GA]^AATT[CT]
370 GAATTC
Alw44I 334 GTGCAC G^TGCAC
1441 GTGCAC
ApaLI 334 GTGCAC G^TGCAC
1441 GTGCAC
ApoI 117 AAATTT [GA]^AATT[CT]
370 GAATTC
AspHI 334 GTGCAC G[AT]GC[AT]^C
1441 GTGCAC
AvaII 432 GGACC G^G[AT]CC
864 GGTCC
BsaWI 296 TCCGGT [AT]^CCGG[AT]
1375 ACCGGA
BsiHKAI 334 GTGCAC G[AT]GC[AT]^C
1441 GTGCAC
BsmI 165 GAATGC GAATGC(1/-1)
1552 GCATTC
BspHI 1416 TCATGA T^CATGA
1605 TCATGA
BsrFI 414 GCCGGC [GA]^CCGG[CT]
938 ACCGGC
BstAPI 902 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
986 GCAGAACCTGC
BstYI 204 GGATCT [GA]^GATC[CT]
347 AGATCC
Cfr10I 414 GCCGGC [GA]^CCGG[CT]
938 ACCGGC
FauI 83 CCCGC CCCGC(4/6)
1354 GCGGG
HindIII 596 AAGCTT A^AGCTT
1815 AAGCTT
MaeII 1031 ACGT A^CGT
1287 ACGT
MflI 204 GGATCT [GA]^GATC[CT]
347 AGATCC
NlaIV 843 GGCCCC GG[ACGT]^[ACGT]CC
972 GGCTCC
RcaI 1416 TCATGA T^CATGA
1605 TCATGA
TaqI 1028 TCGA T^CGA
1338 TCGA
TfiI 1056 GAATC G^A[AT]TC
1705 GAATC
TthHB8I 1028 TCGA T^CGA
1338 TCGA
XcmI 858 CCACATGGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1459 CCAGCCTGGGAGTGG
XhoII 204 GGATCT [GA]^GATC[CT]
347 AGATCC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BbvI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NcoI NdeII
NlaIII PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I Tsp509I TspRI