Restriction map for
pF1KB4088
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
ApaLI AscI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrGI BssHII BstAPI BstBI BstUI
BstXI Bsu36I CelII ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FseI FspI HgaI Hin1I
KasI KspI MluI MluNI MroI
MscI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 1459 GCGG CCGC(-3/-1)
Aor51HI 583 AGCGCT AGC^GCT
AseI 1444 ATTAAT AT^TAAT
AsnI 1444 ATTAAT AT^TAAT
BbsI 2098 GTCTTC GAAGAC(2/6)
BciVI 1405 GGATAC GTATCC(6/5)
BcnI 62 CCCGG CC^[GC]GG
BpuAI 2098 GTCTTC GAAGAC(2/6)
BsaAI 2065 TACGTA [CT]AC^GT[GA]
BsaBI 484 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 328 GAGGAG GAGGAG(10/8)
BsrDI 1133 GCAATG GCAATG(2/0)
BsrFI 421 ACCGGC [GA]^CCGG[CT]
Bst1107I 1506 GTATAC GTA^TAC
BstZ17I 1506 GTATAC GTA^TAC
CfoI 584 GCGC GCG^C
Cfr10I 421 ACCGGC [GA]^CCGG[CT]
DrdI 1999 GACAGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1706 CCATGG C^C[GA][CT]GG
EarI 381 GAAGAG CTCTTC(1/4)
Eco47III 583 AGCGCT AGC^GCT
EcoT14I 1706 CCATGG C^C[AT][AT]GG
EcoT22I 1229 ATGCAT ATGCA^T
HaeII 583 AGCGCT [GA]GCGC^[CT]
HhaI 584 GCGC GCG^C
HinP1I 584 GCGC G^CGC
HincII 871 GTTAAC GT[CT]^[GA]AC
HindII 871 GTTAAC GT[CT]^[GA]AC
HindIII 816 AAGCTT A^AGCTT
HpaI 871 GTTAAC GTT^AAC
Ksp632I 381 GAAGAG CTCTTC(1/4)
MamI 484 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 827 CAATTG C^AATTG
MspA1I 1766 CAGCTG C[AC]G^C[GT]G
MunI 827 CAATTG C^AATTG
NciI 62 CCCGG CC^[GC]GG
NcoI 1706 CCATGG C^CATGG
NdeI 452 CATATG CA^TATG
NsiI 1229 ATGCAT ATGCA^T
PflMI 1388 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1444 ATTAAT AT^TAAT
PvuII 1766 CAGCTG CAG^CTG
SexAI 632 ACCTGGT A^CC[AT]GGT
SnaBI 2065 TACGTA TAC^GTA
StyI 1706 CCATGG C^C[AT][AT]GG
Van91I 1388 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 699 CCATGCTAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1468 GGTACC G^GTACC
1699 GGTACC
AccI 620 GTAGAC GT^[AC][GT]AC
1506 GTATAC
AflIII 1084 ACATGT A^C[GA][CT]GT
2094 ACATGT
ApaI 1217 GGGCCC GGGCC^C
1236 GGGCCC
Asp718I 1468 GGTACC G^GTACC
1699 GGTACC
AvaII 696 GGTCC G^G[AT]CC
849 GGACC
BclI 549 TGATCA T^GATCA
599 TGATCA
BsaWI 1255 TCCGGT [AT]^CCGG[AT]
1545 TCCGGT
BsmI 1983 GAATGC GAATGC(1/-1)
22 GCATTC
BspHI 2009 TCATGA T^CATGA
2033 TCATGA
BspLU11I 1084 ACATGT A^CATGT
2094 ACATGT
BstEII 916 GGTGACC G^GT[ACGT]ACC
1095 GGTAACC
BstPI 916 GGTGACC G^GT[ACGT]ACC
1095 GGTAACC
DraI 526 TTTAAA TTT^AAA
1425 TTTAAA
EcoO65I 916 GGTGACC G^GT[ACGT]ACC
1095 GGTAACC
FbaI 549 TGATCA T^GATCA
599 TGATCA
KpnI 1468 GGTACC GGTAC^C
1699 GGTACC
MslI 741 CACTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2034 CATGAGAGTG
RcaI 2009 TCATGA T^CATGA
2033 TCATGA
SfaNI 1228 GATGC GCATC(5/9)
1542 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BanI BanII BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII