Restriction map for
pF1KB3666
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccI AccII AccIII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HgaI KpnI KspI MamI
MluI MroI MslI MvnI NaeI
NdeI NgoMIV NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 37 GACGTC GACGT^C
AclI 451 AACGTT AA^CGTT
AflII 433 CTTAAG C^TTAAG
AvaI 1919 CTCGGG C^[CT]CG[GA]G
BalI 866 TGGCCA TGG^CCA
BbeI 55 GGCGCC GGCGC^C
BbvCI 1696 GCTGAGG CCTCAGC(-5/-2)
BclI 507 TGATCA T^GATCA
BfrI 433 CTTAAG C^TTAAG
BsaI 180 GAGACC GGTCTC(1/5)
BsmFI 143 GGGAC GGGAC(10/14)
BsmI 1778 GAATGC GAATGC(1/-1)
BsoBI 1919 CTCGGG C^[CT]CG[GA]G
BssSI 1625 CACGAG CACGAG(-5/-1)
BstYI 648 AGATCC [GA]^GATC[CT]
DraII 143 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1187 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 866 TGGCCA [CT]^GGCC[GA]
EcoO109I 143 GGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 507 TGATCA T^GATCA
HincII 772 GTTAAC GT[CT]^[GA]AC
HindII 772 GTTAAC GT[CT]^[GA]AC
HindIII 334 AAGCTT A^AGCTT
HpaI 772 GTTAAC GTT^AAC
KasI 55 GGCGCC G^GCGCC
MfeI 1502 CAATTG C^AATTG
MflI 648 AGATCC [GA]^GATC[CT]
MluNI 866 TGGCCA TGG^CCA
MscI 866 TGGCCA TGG^CCA
MunI 1502 CAATTG C^AATTG
NarI 55 GGCGCC GG^CGCC
NcoI 716 CCATGG C^CATGG
NheI 1596 GCTAGC G^CTAGC
PflMI 1186 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 143 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 451 AACGTT AA^CGTT
SapI 1312 GAAGAGC GCTCTTC(1/4)
SfoI 55 GGCGCC GGC^GCC
TaqI 360 TCGA T^CGA
TthHB8I 360 TCGA T^CGA
Van91I 1186 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1308 TCTAGA T^CTAGA
XhoII 648 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 37 GACGTC G[GA]^CG[CT]C
55 GGCGCC
AlwNI 1892 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2108 CAGAATCTG
AocI 514 CCTAAGG CC^T[ACGT]AGG
1264 CCTGAGG
Asp700I 421 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1336 GAACTGGTTC
AspHI 114 GAGCTC G[AT]GC[AT]^C
541 GAGCTC
BcnI 176 CCCGG CC^[GC]GG
2196 CCGGG
BsaHI 37 GACGTC G[GA]^CG[CT]C
55 GGCGCC
BsiHKAI 114 GAGCTC G[AT]GC[AT]^C
541 GAGCTC
BspHI 1344 TCATGA T^CATGA
1419 TCATGA
Bsu36I 514 CCTAAGG CC^T[ACGT]AGG
1264 CCTGAGG
DsaI 716 CCATGG C^C[GA][CT]GG
1013 CCGTGG
Eco81I 514 CCTAAGG CC^T[ACGT]AGG
1264 CCTGAGG
EcoT14I 491 CCAAGG C^C[AT][AT]GG
716 CCATGG
EcoT22I 919 ATGCAT ATGCA^T
2017 ATGCAT
FauI 1684 CCCGC CCCGC(4/6)
703 GCGGG
HaeII 6 GGCGCT [GA]GCGC^[CT]
55 GGCGCC
HapII 177 CCGG C^CGG
2196 CCGG
Hin1I 37 GACGTC G[GA]^CG[CT]C
55 GGCGCC
HpaII 177 CCGG C^CGG
2196 CCGG
MspI 177 CCGG C^CGG
2196 CCGG
NciI 176 CCCGG CC^[GC]GG
2196 CCGGG
NsiI 919 ATGCAT ATGCA^T
2017 ATGCAT
RcaI 1344 TCATGA T^CATGA
1419 TCATGA
SacI 114 GAGCTC GAGCT^C
541 GAGCTC
SfcI 500 CTGTAG C^T[GA][CT]AG
1374 CTGTAG
SmlI 270 CTCAAG C^T[CT][GA]AG
433 CTTAAG
SspI 1067 AATATT AAT^ATT
1987 AATATT
StyI 491 CCAAGG C^C[AT][AT]GG
716 CCATGG
XcmI 1149 CCATGAGACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1427 CCATAGTTGAAGTGG
XmnI 421 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1336 GAACTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI